Elevation of Bcl2 expression in artery spiralis of pregnant Rattus norvegicus infected with Tachyzoite of Toxoplasma gondii with hyperbaric oxygen therapy

Arif Rahman Nurdianto, Aryati Aryati, Mohammad Guritno Suryokusumo, Mufasirin Mufasirin

Abstract

ABSTRACT

Spiral artery apoptosis plays a role in the process of abortion. Low Bcl2 expression found in abortion and in spiral artery. In pregnancy, Toxoplasmosis infection is found to increase apoptosis in spiral arteries. Hyperbaric Oxygen Therapy (HBOT) improves the expression of Bcl-2. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of Bcl2 in artery spiralis of pregnant rats infected with tachyzoite of Toxoplasma gondii. This is an experiment with a ‘randomized control group of post-test only design’ on 37 Rattus novergicus Sprague Dawley rats. Rats were divided into four groups. The group A is pregnant rats infected with 103 tachyzoites via intraperitoneal injection and received 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is pregnant only and received HBOT. Group C is pregnant and infected with tachyzoite but did not received HBOT. And the last, Group D is pregnant rats with no infection and did not received HBOT. Examinations of Bcl2 expressions were performed on day-5 after HBOT (twice a day). The Bcl2 expression was measured with immunohistochemistry. All data were tested with One-way ANOVA from SPSS 21.  There is an increased expression of Bcl2 spiralis artery in the Group A. There was a significant difference between Group A and Group C with a value of p=0.042. HBOT can increase the expression of Bcl2 from the spiral arteries of rats, in the provision of HBOT 2.4 ATA for 3x30 minutes, 10 times in 5 days.

Keywords: Toxoplasma gondii, tachyzoite, Hyperbaric Oxygen Therapy, Bcl2, spiralis artery

Correspondence to: didins99@gmail.com

 

 

ABSTRAK

Apoptosis arteri spiralis berperan dalam proses aborsi. Ekspresi Bcl2 rendah ditemukan pada aborsi dan di arteri spiral. Pada kehamilan, infeksi Toxoplasmosis ditemukan meningkatkan apoptosis pada arteri spiralis. Terapi Oksigen Hiperbarik (HBOT) meningkatkan ekspresi Bcl-2. Ini adalah penelitian eksperimental dengan desain post-test only pada 37 hamil Rattus novergicus Sprague Dawley, maka tikus dibagi menjadi 4 kelompok. Grup A HBOT adalah tikus hamil yang terinfeksi tachyzoite yang menerima terapi 10 sesi HBOT 2.4 ATA dalam 3x30 menit; kelompok B adalah Hamil saja dan tidak mendapat HBOT; kelompok C Hamil dan terinfeksi tachyzoite T.gondii tetapi tidak menerima HBOT; dan kelompok D adalah tikus hamil normal. Setiap tikus hamil yang terinfeksi diberi 103Tachyzoite Toxoplasma gondii melalui intraperitoneal. Pemeriksaan ekspresi Bcl2 dilakukan pada hari ke-5 setelah HBOT (dua kali sehari). Tikus terbunuh dan kadar Bcl2 Arteri spiralis diukur dengan pemeriksaan Immunohistokimia. Semua data dianalisis dengan uji ANOVA dengan Program SPSS 21. Pada studi ini menunjukkan terapi Oksigen Hiperbarik dapat meningkatkan Ekspresi Bcl2 Arteri Spiralis dengan dosis 2.4 ATA selama 3x30 menit dalam 10 sesi selama 5 hari terapi. Studi ini menyimpulkan bahwa HBOT dapat meningkatkan ekspresi Bcl2 dalam arteri spiralis, dalam pemberian HBO 2.4 ATA selama 3x30 menit dalam 10 sesi.

Kata kunci: HBOT, Arteri spiralis, Bcl2, Tachyzoite Toxoplasma gondii

Korespondensi: didins99@gmail.com

 

Keywords

Toxoplasma gondii, Tachyzoite, Hyperbaric Oxygen Therapy, Bcl2, Artery spiralis,

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References

Al-Fertosi, Raghed B. and Juma, Ameena S.M. 2006. Possible Cellular Expression of IFNγ in a woman with abortion infected Toxoplasma gondii. Medical Journal of Islamic World Academy of Sciences 16:3, 121-134

Barbosa B.F.,et al 2015. IL10, TGF Beta1, and IFN Gamma Modulate Intracellular Signaling Pathways and Cytokine Production to Control Toxoplasma gondii Infection in BeWo Trophoblast Cells. Biology Reproduction. 92(3):82, 1–13

Clark DA, Ding JW, Chaouat G. 2001. Unexplained Sporadic and Recurrent miscarriage in the New Millenium: a critical analysis of immune mechanisms and treatments. Hum Reprod update 7:501-11

Cunningham FG, MacDonald, Gant, Leveno KJ, Gilstrap LC, Hankins, et al. 2001 Abortion. Dalam: Williams Obstetrics.21st ed. Connecticut: Appleton and Lange. 855-69

Danihel L, Gomolcak P, Korbel M, Pruzinec J, Vojtaššak J, Janik P et al. .2002. Expression of Proliferation and Apoptotic Markers in Human placenta During Pregnancy. Acta Histochem 2002; 104(4):335-8

Dewi BDN, 2009. Efek terapi kombinasi metformin-oksigen hiperbarik pada

pasien diabetes mellitus tipe 2 : peningkatan kadar endothelial nitric oxide

synthase (eNOS) dan penurunan kadar glukosa darah, Tesis, Universitas

Airlangga Surabaya

De Falco M, Fedele V, Cobellis L, Mastrogiacomo A. Leone S, Giraldi D, De Luca B, Laforgia V, De Luca A. 2004. Immunohistochemical distribution of proteins belonging to the receptor-mediated and the mitochondrial apoptotic pathways in human placenta during gestation. Cell Tissue Res 2004. 318: 599-608

Dockrell DH. 2003. The multiple roles of Fas ligand in the pathogenesis of infectious diseases. Clin Microbiol Infect; 9:766-79

Hill JA. 2004. Recurrent pregnancy loss. In: Creasy RK, Resnik R, editor. Maternal-fetal medicine principle and practice. . Philadelphia: Saunders 2004: 579-601

Hockenberry D, Nuñez G, Milliman C, Schreiber RD, Korsmeyer SJ. 1990. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348(6299):334-6.

Kupesic S, Kurjak A, Aksamija A. 2002. Threatened abortion: new insights by color Doppler, three-and four-dimensional ultrasound. In: Chervenak FA, Kurjak A, Papp Z, editor. The fetus as a patient: the evolving challenge. New York, London: Parthenon Publishing: 23-38.

Lea RG, Riley SC, Antipatis C, Hannah L, Ashworth CJ, Clark DA, Critchley HOD. 1999. Cytokine and regulation of apoptosis in reproductive tissue: a review. Am J Reprod Immunol; 42:100-9

Liu Z, Jiao QF, You C, Che YJ, Su FZ. 2006. Effect of hyperbaric oxygen on cytochrome C, Bcl-2 and Bax expression after experimental traumatic brain injury in rats. Chin J Traumatol 9:168-174.

Long, Y et al. 2014. Hyperbaric oxygen therapy reduce apoptosis after spinal cord injury in rats. Int J Clin Exp Med.; 7(11): 4073–4081. Taken from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276174/

Murray RK. 2003. Kanker, gen kanker, dan faktor pertumbuhan. Dalam: Murray RK, Granner DK, Mayes PA, Rodwell VW. Biokimia Harper. Edisi 25. Jakarta: EGC; 750-73

Novak, M., Madej, J.A., Dziegeil, P. 2007.The intensity of Cox 2 expression in Cell of Soft Tissue Fibrosarcomas in Dog As Related to Grade of Tumor malignation. Bull Vet Inst Pulawy 51, 275-279. 200.

Nurdianto, A.R., Aryati, Suryokusumo, M.G., Mufasirin. 2018. Effect of hyperbaric oxygen therapy to IFNγ and TNFα expression in pregnant Rattus novergicus infected with Tachyzoite of Toxoplasma gondii. Bali Medical Journal 8(1): 94 100. DOI:10.15562/bmj.v8i1.1316

Palzur E, Zaaroor M, Vlodavsky E, Milman F, Soustiel JF. 2008. Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by Fiftpreservation of mitochondrial membrane properties. Brain Res 1221:126-133.

Prawirohardjo S. 2009. Ilmu Kebidanan. In Jakarta: PT. Bina Pustaka Sarwono Prawirohardjo

Reed JC. Foreword. Oncogene 2008; 27:6192-3

Rusdiana, Dina. 2014. Efek Oksigen Hiperbarik Terhadap Peningkatan Ekspresi Akt pada Rattus Novergicus dengan Diabetes Mellitus. Tesis, Universitas Airlangga

Satoh MS, Lindahl T. Role of poly (ADP-ribose) formation in DNA repair. Nature.1992; 356(6367): 356-8

Savion S, Lepsky E, Orenstein H, Carp H, Shepshelovich J, Torchinsky A, et al. 2002. Apoptosis in the uterus of mice with pregnancy loss. Am J Reprod Immunol.; 47:118-27

Stirrat GM,et al. 2001. High-risk pregnancy management option. Second edition. London: WB. Saunder : 91-110.

Sun, S.Y. et al. 2001.Overexpression of Bcl2 Blocks TNF-Related Apoptosis-Inducing Ligand (TRAIL)-Induced Apoptosis in Human Lung Cancer Cells. Biochemical and Biophysical Research Communications Volume 280, Issue 3, 26 January 2001, Pages 788-797. accessed from https://www.sciencedirect.com/science/article/pii/S0006291X00942189

Sun QH, Peng JP, Xia HF, Yang Y. 2007. IFN-γ Promotes Apoptosis of the Uterus and Placenta in Pregnant Rat and Human Cytotrophoblast Cells. Journal of Interferon & Cytokine Research; 27:567-78

Toder V, Carp H, Fein A, Torchinsky A. 2002. The role of pro and anti-apoptotic molecular interactions in embryonic maldevelopment. Am J Reprod Immunol; 48:235-44

Torchinsky A, Toder V. 2004. To Die or Not to Die: the Function of the Transcription Factor NF-kB in Embryos Exposed to stress. Am J Reprod Immunol; 51:138-43

Vlodavsky E, Palzur E, Soustiel JF .2006. Hyperbaric oxygen therapy reduces neuroinflammation and expression of matrix metalloproteinase-9 in the rat model of traumatic brain injury. Neuropathol Appl Neurobiol 32:40-50.

Weiss JL, Malone FD, Vidaver J, Ball RH, Nyberg DA, Comstock CH, et al. 2004. Threatened abortion: a risk factor poor pregnancy outcome, a population-based screening study. Am J Obstet Gynecol; 190:745-50

Whitley G. S.J and Cartwright J.E. 2010. Cellular and Molecular Regulation of Spiral Artery Remodelling: Lessons from the Cardiovascular Field. Placenta. 2010 Jun; 31(6): 465–474. Taken from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2882556/

Xiao et al. 2015. Synergistic effect of hyperbaric oxygen preconditioning and hydrogen-rich saline in ameliorating rat flap ischemia/reperfusion injury. Plast Aesthet Res.;2:332-9cTaken from https://parjournal.net/article/view/1240

Xing P., Ma. K., Li L., Wang D., Hu G., Long W. 2018. The protection effect and mechanism of hyperbaric oxygen therapy in rat brain with traumatic injury. Acta Cir Bras. 2018;33(4):341-353

Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W .2012. Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy. Neural Regen Res 7:1318-1324..

Zhang Y, Yang Y, Tang H, Sun W, Xiong X, Smerin D, Liu J. 2014. Hyperbaric oxygen therapy ameliorates local brain metabolism, brain edema and inflammatory response in a blast-induced traumatic brain injury model in rabbits. Neurochem Res.; 39:950-960.


DOI: http://dx.doi.org/10.30651/jqm.v3i2.2706