Amplification genetic engineering strategy by Crispr-Cas13 Enzymes for detection and treatment COVID-19 mediated with gold nanoparticle (AUNP)

Salsabila Farah Rafidah (1), Ilham Rahmanto (2), Ardellia Bertha Pratiska (3)
(1) Universitas Airlangga, Indonesia,
(2) ,
(3)

Abstract

WHO declared the disease outbreak due to the COVID-19 coronavirus a global pandemic. Indonesian government's efforts to eradicate the pandemic through mass screening have not been effective due to the limitations of the three main modalities used to detect COVID-19, including Rapid Test Diagnostic (RTD) antibodies, RTD antigens, and Reverse Transcriptase-Polymerase Chain Reaction (RT-RTD PCR), In addition, other detection tools are sometimes used, such as Enzyme-Linked Immunosorbent Assay (ELISA) and rapid molecular tests. To eradicate this pandemic, the government needs COVID-19 detection tools that are effective, cheap, fast, and accessible. To determine the application of the genetic engineering strategy of amplification by the CRISPR-cas13 enzyme for detecting and treating COVID-19 mediated by gold nanoparticles (AuNP). This research uses a qualitative literature study with content analysis, observation development, and literature study; an alternative solution to this problem is CRISPR-Cas13, achieved by the SHERLOCK method. This method designs and screens a targeted group of CRISPR RNAs based on the identification of functional crRNAs of SARS-CoV-2. Amplification of CRISPR-Cas13 by SHERLOCK and PAC-MAN enzymes has the potential to be the latest detection and treatment method for gold nanoparticle-mediated COVID-19 (AuNP) in Indonesia.

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References

Abbott, Timothy R., Girija Dhamdhere, Yanxia Liu, Xueqiu Lin, Laine Goudy, Leiping Zeng, Augustine Chemparathy, Stephen Chmura, Nicholas S. Heaton, Robert Debs, Tara Pande, Drew Endy, Marie F. La Russa, David B. Lewis, and Lei S. Qi. 2020. “Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza.†Cell 181(4):865-876.e12.

Anderson, Caleb F., Maria E. Grimmett, Christopher J. Domalewski, and Honggang Cui. 2020. “Inhalable Nanotherapeutics to Improve Treatment Efficacy for Common Lung Diseases.†Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 12(1).

Draz, Mohamed Shehata, and Hadi Shafiee. 2018. “Applications of Gold Nanoparticles in Virus Detection.†Theranostics 8(7):1985–2017.

Geiser, Marianne, Oliver Quaile, Alexander Wenk, Christoph Wigge, Sylvie Eigeldinger-Berthou, Stephanie Hirn, Martin Schäffler, Carsten Schleh, Winfried Möller, Marcus A. Mall, and Wolfgang G. Kreyling. 2013. “Cellular Uptake and Localization of Inhaled Gold Nanoparticles in Lungs of Mice with Chronic Obstructive Pulmonary Disease.†Particle and Fibre Toxicology 10(1):1–10.

Gootenberg, Jonathan S., Omar O. Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler, Aaron J. Dy, Julia Joung, Vanessa Verdine, Nina Donghia, Nichole M. Daringer, Catherine A. Freije, Cameron Myhrvold, Roby P. Bhattacharyya, Jonathan Livny, Aviv Regev, Eugene V. Koonin, Deborah T. Hung, Pardis C. Sabeti, James J. Collins, and Feng Zhang. 2017. “Nucleic Acid Detection with CRISPR-Cas13a/C2c2.†Science 356(6336):438–42.

Handayani, Diah et al. 2002. “Penyakit Virus Corona 2019.†CPD Infection 3(1):9–12.

Instiaty, I. Gusti Agung Ayu Putu Sri Darmayani, Jefman Efendi Marzuki, Ferina Angelia, William, Angelina Siane, Lela Dwi Sary, Lina Yohanes, Reni Widyastuti, Riki Nova, Dewi Sharon Simorangkir, Lonah, Yolanda Safitri, Gestina Aliska, and Anggi Gayatri. 2020. “Antiviral Treatment of Covid-19: A Clinical Pharmacology Narrative Review.†Medical Journal of Indonesia 29(3):332–45.

Lotfi, Melika, and Nima Rezaei. 2020. “CRISPR/Cas13: A Potential Therapeutic Option of COVID-19.†Biomedicine and Pharmacotherapy 131(June):110738.

Metsky, Hayden C., Nicole L. Welch, Nicholas J. Haradhvala, Laurie Rumker, Yibin B. Zhang, Priya P. Pillai, David K. Yang, Cheri M. Ackerman, Juliane Weller, Paul C. Blainey, Cameron Myhrvold, Michael Mitzenmacher, and Pardis C. Sabeti. 2020. “Efficient Design of Maximally Active and Specific Nucleic Acid Diagnostics for Thousands of Viruses.†BioRxiv 2020.11.28.401877.

Mustafa, Mujahed I., and M. Makhawi. 2021. “Crossm SHERLOCK and DETECTR : CRISPR-Cas Systems as Potential.†Journal of Clinical Microbiology 59(3):1–10.

Myhrvold, Cameron, Catherine A. Freije, Jonathan S. Gootenberg, Omar O. Abudayyeh, Hayden C. Metsky, Ann F. Durbin, Max J. Kellner, Amanda L. Tan, Lauren M. Paul, Leda A. Parham, Kimberly F. Garcia, Kayla G. Barnes, Bridget Chak, Adriano Mondini, Mauricio L. Nogueira, Sharon Isern, Scott F. Michael, Ivette Lorenzana, Nathan L. Yozwiak, Bronwyn L. MacInnis, Irene Bosch, Lee Gehrke, Feng Zhang, and Pardis C. Sabeti. 2018. “Field-Deployable Viral Diagnostics Using CRISPR-Cas13.†Science 360(6387):444–48.

Ophinni, Youdiil, Anshari Saifuddin Hasibuan, Alvina Widhani, Suzy Maria, Sukamto Koesnoe, Evy Yunihastuti, Teguh H. Karjadi, Iris Rengganis, and Samsuridjal Djauzi. 2020. “COVID-19 Vaccines: Current Status and Implication for Use in Indonesia.†Acta Medica Indonesiana 52(4):388–412.

Patchsung, Maturada, Krittapas Jantarug, Archiraya Pattama, Kanokpol Aphicho, Surased Suraritdechachai, Piyachat Meesawat, Khomkrit Sappakhaw, Nattawat Leelahakorn, Theerawat Ruenkam, Thanakrit Wongsatit, Niracha Athipanyasilp, Bhumrapee Eiamthong, Benya Lakkanasirorat, Thitima Phoodokmai, Nootaree Niljianskul, Danaya Pakotiprapha, Sittinan Chanarat, Aimorn Homchan, Ruchanok Tinikul, Philaiwarong Kamutira, Kochakorn Phiwkaow, Sahachat Soithongcharoen, Chadaporn Kantiwiriyawanitch, Vinutsada Pongsupasa, Duangthip Trisrivirat, Juthamas Jaroensuk, Thanyaporn Wongnate, Somchart Maenpuen, Pimchai Chaiyen, Sirichai Kamnerdnakta, Jirawat Swangsri, Suebwong Chuthapisith, Yongyut Sirivatanauksorn, Chutikarn Chaimayo, Ruengpung Sutthent, Wannee Kantakamalakul, Julia Joung, Alim Ladha, Xin Jin, Jonathan S. Gootenberg, Omar O. Abudayyeh, Feng Zhang, Navin Horthongkham, and Chayasith Uttamapinant. 2020. “Clinical Validation of a Cas13-Based Assay for the Detection of SARS-CoV-2 RNA.†Nature Biomedical Engineering 4(12):1140–49.

Rojanarat, W., T. Nakpheng, E. Thawithong, N. Yanyium, and T. Srichana. 2012. “Inhaled Pyrazinamide Proliposome for Targeting Alveolar Macrophages.†Drug Delivery 19(7):334–45.

Shihong Gao, David, Xiaodong Zhu, and Binfeng Lu. 2021. “Development and Application of Sensitive, Specific, and Rapid CRISPR-Cas13-Based Diagnosis.†Journal of Medical Virology 93(7):4198–4204.

Wahjudi, Mariana. 2020. “Kontroversi Metode Deteksi COVID-19 Di Indonesia.†KELUWIH: Jurnal Kesehatan Dan Kedokteran 2(1):32–42.

Wang, Xiaohua, Qingsong Lai, Baoqing Zheng, Li Ye, Si Wen, Yunling Yan, Bin Yang, and Mao Qiang Man. 2021. “Both Prevalence and Severity of Pruritus Are Associated with Age in Chinese Patients with Skin Diseases.†Clinical, Cosmetic and Investigational Dermatology 14:217–23.

Yanti, Budi, Fitri Dewi Ismida, and Klarina Elsa Siti Sarah. 2020. “Perbedaan Uji Diagnostik Antigen, Antibodi, RT-PCR Dan Tes Cepat Molekuler Pada Coronavirus Disease 2019.†Jurnal Kedokteran Syiah Kuala 20(3):172–77.

Authors

Salsabila Farah Rafidah
salsabila.farah.rafidah-2018@fkm.unair.ac.id (Primary Contact)
Ilham Rahmanto
Ardellia Bertha Pratiska
Rafidah, S. F., Rahmanto, I., & Pratiska, A. B. (2022). Amplification genetic engineering strategy by Crispr-Cas13 Enzymes for detection and treatment COVID-19 mediated with gold nanoparticle (AUNP). Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya, 6(2). https://doi.org/10.30651/jqm.v6i2.11227

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