Larvicide activity of ethanol extracts from Zodia (Evodia suaveolens S.) leaves and Cananga (Cananga odorata) flowers on Aedes aegypti larvae
Abstract
Controlling Aedes aegypti population in Dengue management programs is often carried out using high doses of synthetic insecticides, such as temephos, that may lead to resistances. An alternative of natural ingredient such as Zodia (Evodia suaveolens S.) leaves, that containing evodiamine and Cananga (Cananga odorata) flower that containing linalool, appears to be a promising larvicide. This study aims to determine the effectivity of ethanol extracts from Zodia leaves and Cananga flowers on the mortality of Aedes aegypti larvae. This experimental study using Aedes aegypti larvae at stage of III-IV. Each ingredient was extracted by the maceration method and then prepared at concentration of 2.5% and 5%. PEG 400 was also added as a diluent. Observations performed every 6 hours, for 24 hours. Data analysis using Kruskal Wallis test and Dunn's test. Within 24 hours, in each of study group, there was 100% larval mortality. The Kruskal Wallis test resulting p-value of <0.05. From Dunn’s test, comparisons between each study group and the negative and positive control groups yielded p-values <0.05 and 1.000, respectively. Thus, it can be said that ethanol extracts from Zodia leaves and Cananga flowers are as effective as temephos as larvicide on Aedes aegypti larvae
References
Akbar, S. (2020). Handbook of 200 Medicinal Plants. Springer International Publishing. https://doi.org/10.1007/978-3-030-16807-0
Chaiphongpachara, T., & Laojun, S. (2020). Comparative efficacy of commercial ylang-ylang (Cananga odorata) essential oils from India and Thailand against larval Aedes aegypti (L.) (Diptera: Culicidae). Journal of Advanced Veterinary and Animal Research, 7(3), 391. https://doi.org/10.5455/javar.2020.g433
Chakira, A., Garcia, C., Soria, C., Minier, J., & Chillet, M. (2022). Effect of Flower Development Stages on the Dynamics of Volatile Compounds in Ylang-Ylang (Cananga odorata) Essential Oil. Horticulturae, 8(11), 986. https://doi.org/10.3390/horticulturae8110986
de Souza Wuillda, A. C. J., Campos Martins, R. C., & Costa, F. das N. (2019). Larvicidal Activity of Secondary Plant Metabolites in Aedes aegypti Control: An Overview of the Previous 6 Years. Natural Product Communications, 14(7), 1934578X1986289. https://doi.org/10.1177/1934578X19862893
Dewi, L. M., Ariffah, H. Z., Aisyah, R., & Nurhayani, N. (2023). Bio-larvicidal Potential of Betel Leaves (Piper betle L) Ethanolic Extract in Addition of PEG 400 Diluent on Aedes aegypti Larvae. Biology, Medicine, & Natural Product Chemistry, 12(2), 451–455. https://doi.org/10.14421/biomedich.2023.122.451-455
Dinno, A. (2015). Nonparametric Pairwise Multiple Comparisons in Independent Groups using Dunn’s Test. The Stata Journal: Promoting Communications on Statistics and Stata, 15(1), 292–300. https://doi.org/10.1177/1536867X1501500117
Huang, Y., Lin, M., Jia, M., Hu, J., & Zhu, L. (2020). Chemical composition and larvicidal activity against Aedes mosquitoes of essential oils from Arisaema fargesii. Pest Management Science, 76(2), 534–542. https://doi.org/10.1002/ps.5542
Iriani, F., & Yanuastri, P. W. (2020). Diversity and phytochemistry analysis in zodia plants organs (Evodia suaveolens). IOP Conference Series: Earth and Environmental Science, 458(1), 012019. https://doi.org/10.1088/1755-1315/458/1/012019
Lanner, J. T., Georgiou, D. K., Joshi, A. D., & Hamilton, S. L. (2010). Ryanodine Receptors: Structure, Expression, Molecular Details, and Function in Calcium Release. Cold Spring Harbor Perspectives in Biology, 2(11), a003996–a003996. https://doi.org/10.1101/cshperspect.a003996
Liu, J., Guo, B., Zhong, S., Shi, Y., Li, Z., Yu, Z., Hao, Z., Zhang, L., Li, F., Wang, Y., & Li, Y. (2024). Novel Evodiamine-Based Sulfonamide Derivatives as Potent Insecticide Candidates Targeting Insect Ryanodine Receptors. Journal of Agricultural and Food Chemistry, 72(2), 1292–1301. https://doi.org/10.1021/acs.jafc.3c05680
Liu, J., Shi, Y., Chen, S., Li, F., Wen, W., & Wang, Y. (2022). Discovery of evodiamine derivatives as potent insecticide candidates. Bioorganic & Medicinal Chemistry, 62, 116727. https://doi.org/10.1016/j.bmc.2022.116727
Martínez-Mercado, J. P., Sierra-Santoyo, A., Verdín-Betancourt, F. A., Rojas-García, A. E., & Quintanilla-Vega, B. (2022). Temephos, an organophosphate larvicide for residential use: a review of its toxicity. Critical Reviews in Toxicology, 52(2), 113–124. https://doi.org/10.1080/10408444.2022.2065967
Palomino, M., Pinto, J., Yañez, P., Cornelio, A., Dias, L., Amorim, Q., Martins, A. J., Lenhart, A., & Lima, J. B. P. (2022). First national-scale evaluation of temephos resistance in Aedes aegypti in Peru. Parasites and Vectors, 15(1). https://doi.org/10.1186/s13071-022-05310-x
Puspita, P. A. A., Arpiwi, N. L., & Sudatri, N. W. (2022). Daya proteksi minyak atsiri bunga kenanga (Cananga odorata ) dalam sediaan lotion antinyamuk terhadap Aedes aegypti. Jurnal Biologi Udayana, 26(2), 269. https://doi.org/10.24843/JBIOUNUD.2022.v26.i02.p12
Saeung, M., Ngoen-Klan, R., Thanispong, K., Muenworn, V., Bangs, M. J., & Chareonviriyaphap, T. (2020). Susceptibility of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) to Temephos in Thailand and Surrounding Countries. Journal of Medical Entomology, 57(4), 1207–1220. https://doi.org/10.1093/jme/tjaa035
Sarma, R., Adhikari, K., Mahanta, S., & Khanikor, B. (2019). Insecticidal Activities of Citrus Aurantifolia Essential Oil Against Aedes aegypti (Diptera: Culicidae). Toxicology Reports, 6, 1091–1096. https://doi.org/10.1016/j.toxrep.2019.10.009
Setiyadi, D., Martini, M., & Hadi, M. (2020). Efikasi ekstrak daun zodia (Evodia suaveolens) pelarut heksana dalam mematikan larva instar III Aedes aegypti. Care : Jurnal Ilmiah Ilmu Kesehatan, 8(2), 195. https://doi.org/10.33366/jc.v8i2.1675
Sutrisna, E. (2016). Herbal Medicine : Suatu Tinjauan Farmakologis. Muhammadiyah University Press.
Syed Ismail, S. N. A., Syed Soffian, S. S., Aziz, R. A., & Tahiruddin, N. S. M. (2020). Antibacterial and Insect-Repellent Activities of Cananga odorata Essential Oil. In Charting the Sustainable Future of ASEAN in Science and Technology (pp. 297–306). Springer Singapore. https://doi.org/10.1007/978-981-15-3434-8_26
World Health Organization. (2005). Guidelines for Laboratory and Fields Testing of Mosquito Larvicides. https://www.who.int/publications/i/item/WHO-CDS-WHOPES-GCDPP-2005.13
Yabansabra, Y. R., Bebari, S. F., & Simaremare, E. S. (2023). The Effectiveness of Zodia Leaves (Evodia Suaveolens Scheff) Oil as Aedes aegypti L Mosquito Repellent in Papua. Jordan Journal of Pharmaceutical Sciences, 207–216. https://doi.org/10.35516/jjps.v16i2.1321
Yang, X., Quam, M. B. M., Zhang, T., & Sang, S. (2021). Global burden for dengue and the evolving pattern in the past 30 years. Journal of Travel Medicine, 28(8). https://doi.org/10.1093/jtm/taab146
Zuharah, W. F., Yousaf, A., Ooi, K. L., & Sulaiman, S. F. (2021). Larvicidal activities of family Anacardiaceae on Aedes mosquitoes (Diptera: Culicidae) and identification of phenolic compounds. Journal of King Saud University - Science, 33(5), 101471. https://doi.org/10.1016/j.jksus.2021.101471
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