The differences of parasitemia and lungs size in malaria-associated acute respiratory distress syndrome (MA-ARDS) and non-MA-ARDS in mice infected with Plasmodium berghei ANKA

Eka Noviya Fuzianingsih (1), Cyuzuzo Callixte (2), Marselaonety La’lang (3), Dinda Eka Putri (4), Heny Arwati (5), Lucia Tri Suwanti (6)
(1) Universitas Airlangga, Indonesia,
(2) Universitas Airlangga, Indonesia,
(3) Universitas Airlangga, Indonesia,
(4) Universitas Airlangga, Indonesia,
(5) Universitas Airlangga, Indonesia,
(6) Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia, Indonesia

Abstract

Malaria-associated acute respiratory distress syndrome (MA-ARDS) is characterized by extensive infiltration of leukocytes, microhemorrhages, vasogenic edema, changes in lung color, and a significant increase in the weight of the lung. This study was aimed to find out the differences in parasitemia and lung size in MA-ARDSand non-MA-ARDSin mice infected with Plasmodium berghei ANKA. Sixteen male BALB/c mice were infected with P. berghei ANKA, and daily parasitemia was observed on Giemsa-stained tail blood smears. Mice were sacrificed when parasitemia reached ±20%. Simultaneously eight uninfected mice were used as negative control (NEG). The statistical analysis was done using Kruskal Wallis, Mann Whitney U tests, and Spearman correlation test. The results showed that there were significant differences in parasitemia (p=0.001), weight (p=0.001), and lung length (p=0.021) between the MA-ARDS and non-MA-ARDS groups. Comparison of NEG and MA-ARDS resulted in a significant difference in lung size (p=0.05). When non-MA-ARDScompared with NEG groups, it showed a significant difference in lung width (p=0.001). However, there was no significant difference in lung weight and length (p>0.05). Spearman correlation test showed that there was a strong correlation between parasitemia with weight (p=0.000), length (p=0.001), and lung width (p=0.017). The findings indicated that parasitemia played a role in the development of MA-ARDS in mice infected with P. berghei ANKA and influenced the size of the lung.

 

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Authors

Eka Noviya Fuzianingsih
Cyuzuzo Callixte
Marselaonety La’lang
Dinda Eka Putri
Heny Arwati
heny-a@fk.unair.ac.id (Primary Contact)
Lucia Tri Suwanti
Author Biographies

Eka Noviya Fuzianingsih, Universitas Airlangga

Master Program in Immunology, Postgraduate School

Cyuzuzo Callixte, Universitas Airlangga

Master Program in Immunology, Postgraduate School

Marselaonety La’lang, Universitas Airlangga

Master Program in Immunology, Postgraduate School

Dinda Eka Putri, Universitas Airlangga

Master Program in Immunology, Postgraduate School

Heny Arwati, Universitas Airlangga

Department of Medical Parasitology,
Faculty of Medicine

Lucia Tri Suwanti, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia

Department of Veterinary Parasitology
Fuzianingsih, E. N., Callixte, C., La’lang, M., Putri, D. E., Arwati, H., & Suwanti, L. T. (2022). The differences of parasitemia and lungs size in malaria-associated acute respiratory distress syndrome (MA-ARDS) and non-MA-ARDS in mice infected with Plasmodium berghei ANKA. Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya, 6(1). https://doi.org/10.30651/jqm.v6i1.8853

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