The differences of parasitemia in Plasmodium berghei infected mice treated with extract of mango parasite leaves with Artemisinin combination
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Dendrophthoe pentandra, Antimalarial, In vivo, Spleen index, Artemisinin
Abstract
Malaria is a disease transmitted through the bite of a female Anopheles mosquito caused by the Plasmodium parasite. Plasmodium has been reported to become resistant to artemisinin. Thus, the study on the ethanol extract of mango parasite leaves/Dendrophthoe pentandra (MP) on P. berghei-infected BALB/c mice with and without artemisinin combination (MP+A) was conducted. This study is experimental laboratory research with a post-test only design. The percent parasitemia in mice treated with ethanol extract of MP and MP+A leaves decreased. There was no difference in the spleen index in mice given ethanol extract of MP, and MP+A leaves with p = 0.203 and the spleen weight of mice with p = 0.134 (significance: p <0.005). Pearson Correlation test showed a correlation between spleen index with parasitemia and spleen weight with parasitemia; however, there was no correlation between body weight and parasitemia. The ethanol extracts of MP and MP+A leaves had significant antimalarial activity, and the difference in the percent parasitemia between groups was significant but not so far. The spleen index value was not affected by the ethanol extract of MP and MP+A leaves, but the percent parasitemia was affected.
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References
Anita, A., Khotimah, S., & Yanti, A. H. (2014). Aktivitas antibakteri ekstrak daun benalu jambu air (Dendropthoe pentandra (L.) Miq) terhadap pertumbuhan Salmonella typhi. Jurnal Protobiont, 3(2), 268–272. https://doi.org/10.26418/protobiont.v3i2.6834
Baird, J. K., Nalim, M. F. S., Basri, H., Masbar, S., Leksana, B., Tjitra, E., … Wignall, F. S. (1996). Survey of resistance to chloroquine by Plasmodium vivax in Indonesia. Transactions of The Royal Society of Tropical Medicine and Hygiene, 90(4), 409–411. https://doi.org/10.1016/S0035-9203(96)90526-X
Budiarti, M., Maruzy, A., Ratri, N., & Brotojoyo, E. (2020). Aktivitas antimalaria daun gempol (Nauclea orientalis (L.) L) terhadap Plasmodium falciparum. Media Penelitian Dan Pengembangan Kesehatan, 30(2), 135–146. https://doi.org/10.22435/mpk.v30i2.3044
Ferreira, J. F. S., Luthria, D. L., Sasaki, T., & Heyerick, A. (2010). Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules, 15(5), 3135–3170. https://doi.org/10.3390/molecules15053135
Garber, J. C., Barthold, S. W., Barbee, R. W., Bielitzki, J. T., & Clayton, L. A. (2011). Guide for the care and use of laboratory animals (Eighth Edition; C. H. Fletcher, R. Crossgrove, & R. Haycraft, Eds.). Washington, DC: National Academies Press. https://doi.org/10.17226/12910
Hamsidi, R., Widyawaruyanti, A., Hafid, A. F., Ekasari, W., Malaka, M. H., Kasmawati, H., … Sabarudin, S. (2018). Profil fitokimia ekstrak etanol bunga kasumba turate (Carthamus tinctorius L.) yang berpotensi sebagai antimalaria. Pharmauho: Jurnal Farmasi, Sains, Dan Kesehatan, 4(2), 40–42. https://doi.org/10.33772/pharmauho.v4i2.6267
Handayani, T., Marlina, S., & Dewi, R. M. (2008). Efek antiplasmodium ekstrak kulit batang asam kandis (Garcinia parvifolia Miq) yang diberikan secara intraperitoneal pada mencit yang diinfeksi dengan Plasmodium yoelii. Maranatha Journal of Medicine and Health, 8(1), 20–28. Retrieved from https://www.neliti.com/id/publications/149725/efek-antiplasmodium-ekstrak-kulit-batang-asam-kandis-garcinia-parvifolia-miq-yan#cite
Harijanto, P. N. (Ed.). (2014). Buku ajar ilmu penyakit dalam (6th ed.). Jakarta: Interna Publishing.
Ikawati, M., Wibowo, A. E., Octa, N. S., & Adelina, R. (2008). Pemanfaatan benalu sebagai agen antikanker. Yogyakarta: ResearchGate. Retrieved from https://www.researchgate.net/publication/237693207_Pemanfaatan_Benalu_sebagai_Agen_Antikanker
Intan, P. R., Lestari, T. W., & Sani, Y. (2017). Studi histopatologi pasca pemberian ekstrak campuran kulit batang pulai ( Alstonia scholaris L. R. Br.) dan meniran (Phyllanthus niruri L.) pada mencit terinfeksi Plasmodium berghei. Jurnal Kedokteran Yarsi, 25(1), 010–022. https://doi.org/10.33476/jky.v25i1.325
Karney, W. W., & Tong, M. J. (1972). Malabsorption in Plasmodium falciparum malaria. The American Journal of Tropical Medicine and Hygiene, 21(1), 1–5. https://doi.org/10.4269/ajtmh.1972.21.1
Katzung, B. G., Trevor, A. J., & Hall, M. K. (2018). Basic & clinical pharmacology (12th ed.). NewYork: McGraw-Hill Education.
Kementerian Kesehatan. Lampiran peraturan menteri kesehatan Republik Indonesia nomor 5 tahun 2013 tentang pedoman tata laksana malaria. , Pub. L. No. 5, 5 (2013). Indonesia, Indonesia: BN.2013/No.128,kemkes.go.id.
Nindatu, M., Zaini, N. C., Dachlan, Y. P., & Sjafruddin, S. (2008). Efek antimalaria senyawa flavonoid kulit batang cempedak (Artocarpus champeden spreng) pada morfologi dan aktivitas biokimiawi parasit malaria (Universitas Airlangga). Universitas Airlangga, Surabaya. Retrieved from http://repository.unair.ac.id/id/eprint/32788
Noedl, H., Se, Y., Schaecher, K., Smith, B. L., Socheat, D., & Fukuda, M. M. (2008). Evidence of artemisinin-resistant malaria in Western Cambodia. The New England Journal of Medicine, pp. 2619–2620. Massachusetts Medical Society. https://doi.org/10.1056/NEJMc0805011
Shimada, M., Hirose, Y., Shimizu, K., Yamamoto, D. S., Hayakawa, E. H., & Matsuoka, H. (2019). Upper gastrointestinal pathophysiology due to mouse malaria Plasmodium berghei ANKA infection. Tropical Medicine and Health, 47(1), 1–11. https://doi.org/10.1186/s41182-019-0146-9
Sowunmi, A., Gbotosho, G. O., Adedeji, A. A., Fateye, B. A., Sabitu, M. F., Happi, C. T., & Fehintola, F. A. (2007). Effects of acute Plasmodium falciparum malaria on body weight in children in an endemic area. Parasitology Research, 101, 343–349. https://doi.org/10.1007/s00436-007-0494-y
Suberu, J. O., Yamin, P., Leonhard, K., Song, L., Chemat, S., Sullivan, N., … Lapkin, A. A. (2014). The effect of O-methylated flavonoids and other co-metabolites on the crystallization and purification of artemisinin. Journal of Biotechnology, 171(1), 25–33.
https://doi.org/10.1016/j.jbiotec.2013.11.024
Tjahjandarie, T. S., & Tanjung, M. (2015). Lead compound antimalaria dan antioksidan senyawa alkaloid,flavonoid, dan kumarin dari Limonia accidisima L. (Universitas Airlangga). Universitas Airlangga, Surabaya. Retrieved from http://repository.unair.ac.id/id/eprint/63121
Tjokroprawiro, A., Setiawan, P. B., Effendi, C., Santoso, D., & Sogiarto, G. (Eds.). (2015). Buku ajar ilmu penyakit dalam (2nd ed.). Surabaya: Airlangga University Press (AUP).
Wei, S., Ji, H., Yang, B., Ma, L., Bei, Z., Li, X., … Chen, J. (2015). Impact of chrysosplenetin on the pharmacokinetics and anti-malarial efficacy of artemisinin against Plasmodium berghei as well as in vitro CYP450 enzymatic activities in rat liver microsome. Malaria Journal, 14(1), 1–13. https://doi.org/10.1186/s12936-015-0929-3
WHO (Ed.). (2018). World malaria report 2018. Geneva: World Health Organization.
WHO (Ed.). (2021). World malaria report 2021. Geneva: World Health Organization.
Wilairatana, P., Meddings, J. B., Ho, M., Vannaphan, S., & Looareesuwan, S. (1997). Increased gastrointestinal permeability in patients with Plasmodium falciparum malaria. Clinical Infectious Diseases, 24(3), 430–435. https://doi.org/10.1093/clinids/24.3.430
Windasari, T. A. N., Maslachah, L., & Rahardjo, A. P. (2016). Pengaruh paparan artemisinin berulang terhadap diameter pulpa putih dan indeks limpa pada mencit (Mus musculus) yang diinfeksi Plasmodium berghei. Journal of Basic Medical Veterinary, 5(1), 8–15.
Yulianti, R., Dahlia, A., & Ahmad, A. R. (2014). Penetapan kadar flavonoid total dari ekstrak etanolik daun benalu mangga (Dendrophthoe pentandra L. Miq). Jurnal Fitofarmaka Indonesia, 1(1), 14–17. https://doi.org/10.33096/jffi.v1i1.195
Zhou, Z., Tan, H., Li, Q., Li, Q., Wang, Y., Bu, Q., … Zhang, L. (2020). Trichome and artemisinin regulator 2 positively regulates trichome development and artemisinin biosynthesis in Artemisia annua. New Phytologist, 228(3), 932–945. https://doi.org/10.1111/nph.16777
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Qanun Medika by FK UM Surabaya is liscence under Lisensi Creative Commons Atribusi 4.0 Internasional.