2026-06-04T17:12:29Z https://journal.um-surabaya.ac.id/analis/oai

oai:journal.um-surabaya.ac.id:article/977 2022-07-13T07:02:28Z analis:ART

Pengaruh Lama Pemanasan Terhadap Kadar Alkohol Pada Nira Siwalan (Borassus flabellifer) mardiyah, siti  abstractNira is a raw material for the manufacture of liquid brown sugar, palm sugar, and alcoholic beverages. Among these products are the most widely used alcoholic beverage. During this siwalan juice as a beverage consumed fresh, the storage period is relatively short whice for 1-2 days. If after 3 days the beverage is consumed will have a negative impact, to reduce the alcohol content is then made warming. Based on the above background can be taken formulation of the problem "whether there was an effect on levels of alcohol prolonged heating on sap siwalan"?. The purpose of this study was to determine whether there was an effect on levels of alcohol prolonged heating on sap siwalan. This research is a kind of experimental research with the aim to determine the effect of prolonged heating of the alcohol content in the sap siwalan. The sampling technique used was random sampling, totaling 24 samples. Variable work was composed of independent variables and the dependent variable. Alcohol Examination conducted on sap siwalan by doing without heating or 0 minutes, 10 minutes, 20 minutes and 30 minutes, using a number of methods Pycnometer six replication. From these results a decrease in the average alcohol content of sap siwalan on any heating for 10 min, 20 min and 30 min, after using ANOVA statistical test obtained p = 0.000 (p <0.05) can be summed Ha Ho is rejected or accepted which means there is prolonged heating effect on levels of alcohol in the juice siwalan.Key word : Nira, Level of alcohol, prolonger heating  Universitas Muhammadiyah Surabaya 2017-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/977 10.30651/jmlt.v1i1.977 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 9-15 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/977/757 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/978 2022-07-13T07:03:15Z analis:ART

Perbedaan Hasil Pemeriksaan Kadar Glukosa Darah 2 Jam PP Dengan Menggunakan Glukometer Dan Analyzer Pada Penderita Diabetes Melitus Maulidiyanti, Ellies Tunjung Sari  ABSTRACT Examination of blood glucose levels 2 hours pp is required to diagnose diabetes mellitus. Currently many clinical laboratories, hospitals and clinics are still modest usually use tool for the examination of blood glucose levels is by glucometer that emit relatively fast results. In contrast to the large hospital and clinical laboratories that have been developed have used the analyzer tool as an advanced tool that can check more parameters clinical examination which will get accurate results. To the authors want to prove whether there are differences in the results of blood glucose levels 2 hours pp using glucometer and analyzer. This research is experimental. The population in this study were patients with diabetes mellitus in hospital Throughout Siti Khadija. The samples used were serum and whole blood from the blood vena.Variabel free is the way 2-hour blood glucose tests using a glucometer and analyzer pp and the dependent variable is the blood glucose levels 2 hours pp. While the operational definition, examination of blood glucose 2 hours pp by using 2 kinds of tools, namely glucometer and analyzer. Methods of data collection of 20 samples taken from patients with DM in Hospital Khodijah Throughout Siti. To know the results used paired t test. The results showed that there were significant differences between the glucometer and glucose analyzer examination 2 hours pp based on the results of paired t test with sig value greater than 0.05 and the average levels of the glucometer 207.65 mg / dl and analyzer 193.85 mg / dl. Key word: glucose, glucometer, analyzer  Universitas Muhammadiyah Surabaya 2017-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/978 10.30651/jmlt.v1i1.978 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 16-22 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/978/776 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/979 2022-07-13T07:03:41Z analis:ART

Analisa Kadar Protein Pada Teripang (Holothuria argus) Terhadap Lama Perebusan Kunsah, Baterun  ABSTRACTProtein is the most important nutrients in the body, which serves as a new network-forming and maintaining or repairing worn tissue. Protein cucumbers reach 82% of his body. However, the protein can undergo denaturation when heated. Formulation of the research problem is whether there was an effect on long boiling sea cucumbers (Holothuria argus) the protein content. The research objective was to determine whether there is a long effect on the boiling sea cucumbers (Holothuria argus) the protein content.This type of research is experimental. The study population was all kinds of sea cucumbers are caught by fishermen in Kel.Sukolilo Kec.Bulak Surabaya. The research sample is sea cucumber Holothuria argus species caught by fishermen in Kel.Sukolilo Kec.Bulak Surabaya. Research using the 5 treatments with 5 repetitions. Criteria longer boiling is P1 (0 min / control), P2 (15 min), P3 (30 minutes), P4 (45 minutes), and P5 (60 minutes).Data examined protein levels by means of laboratory tests. Based on the laboratory results obtained average amount of 16.948% protein content in boiling 0 minutes, 13.068% in 15 minutes, 11.986% at 30 minutes, 10.652% at 45 minutes, and 8.732% in boiling water 60 minutes.Test results are then tested the statistical ANOVA showed significant values where 0.000 <0.05 (α). It can be concluded that there is a long effect on the boiling sea cucumbers (Holothuria argus) the protein content.Keywords: Sea Cucumber & Protein Levels Universitas Muhammadiyah Surabaya 2017-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/979 10.30651/jmlt.v1i1.979 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 23-30 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/979/777 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/980 2022-07-13T07:06:08Z analis:ART

Pengaruh Pemberian Sari Tebu Terhadap Kadar Kolesterol Darah Pada Mencit Widyastuti, Rahma  abstractSugarcane (Saccharum officinarum L.) is a plant known by the public as a refreshing drink that tastes sweet and the price is quite economical and readily available. Sugarcane contains octacosanol compounds that can lower cholesterol levels in the blood. Excessive cholesterol (hypercholesterolemia) a threat that is feared because as one of the causes of coronary heart disease (CHD) and stroke. The purpose of this study was to analyze the levels of cholesterol in mice, to analyze cholesterol levels in mice after the administration of sugar cane juice, to analyze the effect of sugar cane juice on cholesterol levels in mice. This type of research conducted by a group of experimental methods with populations of mice (Mus musculus) were obtained from farms Bojonegoro. The research sample selected by criteria mice aged 2-3 months, weighing between 20-40 grams of the male sex, the amount of sample used two groups of mice, each group consisted of 16 mice. From the results of blood cholesterol levels in mice after the administration of sugar cane juice, it is known that the average cholesterol level in the control group was 148.8750 mg / dl and value - average blood cholesterol treatment group was 118.2500 mg / dl. Statistically analyzed using a paired t test t value -3.799 obtained with significant value. (p) = 0.001 which is less than 0.05 then there is the effect (Ho is rejected) (t = -2.040 table). So we can conclude there is the effect of sugar cane juice on blood cholesterol levels in mice.Key word : Sugarcane, blood cholesterol levels Universitas Muhammadiyah Surabaya 2017-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/980 10.30651/jmlt.v1i1.980 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 31-37 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/980/778 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/981 2022-07-13T07:06:38Z analis:ART

Pengaruh Hemolisis Terdapat Kadar Serum Glutamate Pyruvate Transaminase (SGPT) Sebagai Salah Satu Parameter Fungsi Hati kahar, hartono  AbstractSGPT examination is one of the important checks on the state of a particular patient, for example, in patients with liver disease, hepatitis, and so forth. The other purpose is to detect the presence of liver damage. So there was an effect in the event of an increase in maupaun drop in levels. And the serum circumstances that do not qualify, such as serum hemolysis, will greatly affect the actual levels of ALT. Based on the research background, the authors formulate the problem as follows: "Is there any effect of hemolysis on SGPT levels as one of the parameters of liver function?". The purpose of this study is to prove whether there is an influence of hemolysis on SGPT levels as one of the parameters of liver function. The sample was 20 samples and 20 samples of hemolysis hemolysis. Location and time of the research conducted at Muhammadiyah Hospital Clinical Laboratory Lamongan ie in January and July. Of the 20 samples analyzed, showing that levels higher than the hemolysis ALT SGPT levels no hemolysis. When the serum through lysis will experience a significant increase. To test the effect of hemolysis on SGPT levels paired t test was used because the data from this study with normally distributed. The results of a paired t test analysis between serum hemolysis and no hemolysis on levels of serum ALT levels showed a significant value 0.00 thus P <0.05 it can be concluded that there is no difference between the levels of ALT SGPT levels of hemolysis with hemolysis. That means there is the influence of hemolysis on SGPT levels as one of the parameters of liver function. Key word : hemolysis, glutamate pyruvate transminase  Universitas Muhammadiyah Surabaya 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/981 10.30651/jmlt.v1i1.981 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 38-46 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/981/798 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/982 2022-07-13T07:07:01Z analis:ART

Pengaruh Perasan Daun Belimbing Wuluh (Averrhoa bilimbi) Terhadap Pertumbuhan Bakteri Escherichia coli Patogen Azizah, Fitrotin  abstract (Averrhoa bilimbi) is one of the plants that can be used as an antibacterial, good flowers, stems, leaves and stems have benefits and efficacy. Chemical constituents of the leaves starfruit are tannins, flavonoids, saponins. The active ingredient in the leaves starfruit is tannin. Escherichia coli is a bacterium that causes diarrhea. From the above discussion, the authors raised the theme of Influence starfruit juice of the leaves on the growth of pathogenic E. coli bacteria. Formulation of the problem researchers is whether there is influence starfruit juice of the leaves on the growth of Escherichia coli pathogens. This study aims to determine the concentration that could inhibit and kill Esherichia coli Escherichia coli. This research is experimental. The sample used is leaf green starfruit not so young in a fresh state taken in the area around the boarding author Sutorejo 11B stay. In this study, the sample size for each treatment as much as 3 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% and C (control). Independent variables are starfruit juice of the leaves, while the dependent variable growth of Escherichia coli. When the study carried out in January and July 2012. Data on the effect of starfruit juice of the leaves on the growth of Escherichia coli tested by laboratory examination and data collection techniques using Chi-Square 0:05. Based on the results it appears that at a concentration of 100% and 90% were able to kill the bacteria Escherichia coli, whereas the inhibitory power ranging from a concentration of 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%. From Chi-Square test was obtained λ2 count <of λ2 table. From this it turns out there leaves starfruit juice effect on the growth of Escherichia coli pathogenic bacteria.Keyword : Leaves starfruit, E. Coli Universitas Muhammadiyah Surabaya 2017-12-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/982 10.30651/jmlt.v1i1.982 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 47-54 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/982/1698 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1008 2022-07-13T07:07:27Z analis:ART

Efektivitas Minyak Atsiri Serai Wangi (Combypogon nardus) Sebagai Insektisida Alami Untuk Kecoa Amerika (Periplaneta americana) Retno Arimurti, Anindita Riesti   ABSTRAK Cockroaches are one of the most common types of insects around our neighborhood, and it is one of the most dangerous insects because some species of cockroaches are known to transmit disease in humans such as tuberculosis, typhoid, asthma, cholera, and hepatitis. Eradication of cockroaches can be done physically and chemically, the use of chemical-based insecticides can cause respiratory problems. In an insecticide drug containing natural ingredients contain essential oils, the content of essential oil compounds serve as an anti-insect drug on citronella. This study aimed to determine the effect of essential oils of citronella fragrance to cockroaches. This type of research was experimental. The population in this study were cockroaches (Periplaneta americana) obtained from the houses of villagers of Tanjung, Sampang, Madura. The total sample was 32 adult stage cockroaches. Data analysis was using free T test with error rate of  5% or 0,05.The results showed that cockroaches stopped moving with an average of 15.75. Based on T test results showed the significant results of 0.00 which was smaller than 0.05 then H0 was rejected Ha was accepted. It can be concluded that the essential oils of citronella fragrance have an effect on the death of cockroaches. Keywords: Cockroach, Essential Oil Of Citronella Fragrance (Cymbopogon nardus) Universitas Muhammadiyah Surabaya 2017-12-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1008 10.30651/jmlt.v1i1.1008 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 55-60 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1008/780 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1009 2022-07-13T07:07:46Z analis:ART

Perbandingan Kadar Glukosa Darah Sebelum Dan Sesudah Minum Kopi Purwaningsih, Nur Vita  ABSTRAKPeminum kopi terbanyak hingga saat ini ditempati oleh usia 25-39 tahun. Namun, pengaruh gaya hidup serta maraknya cafe atau restoran yang mengolah kopi menjadi berbagai minuman menarik membuat presentase remaja dan dewasa muda sebagai konsumen kopi semakin meningkat. Pada tahun 2011, 40% dari usia 18-24 tahun mengkonsumsi kopi setiap hari, meningkat dari 31%  pada tahun 2010. Kopi dapat menurunkan kadar glukosa darah dan mengurangi resiko terkena penyakit lainnya karena adanya chlorogenic acid yang merangsang Glucagon-like-peptida-1(GLP-1). Adapun tujuan penelitian ini untuk mengetahui perbandingan kadar glukosa darah sebelum dan sesudah minum kopi. Penelitian dilakukan dengan cara eksperimental menggunakan sampel darah kapiler dari mahasiswa Prodi Analis sebanyak 30 orang. Metode: Metode yangdigunakan untuk pemeriksaan kadar glukosa darah adalah rapidtest yang dibantu alat glukometer easytouch . Hasil: Hasil rerata kadar glukosa sebelum perlakuan sebesar 94,10 mg/dL ± 8,242 mg/dL  dan  sesudah perlakuan sebesar 86,96 mg/dL ± 4,994 mg/dL. Diskusi : Berdasarkan analisis data menggunakan uji t berpasangan didapatkan nilai P=0.000, sehingga dapat disimpulkan ada perbedaan kadar glukosa darah puasa sesudah mengkonsumsi kopi, bahwa kopi dapat menurunkan kadar glukosa darah sebelum dan sesudah minum kopi.Kata Kunci : Kopi, Glukosa darah Universitas Muhammadiyah Surabaya 2017-12-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1009 10.30651/jmlt.v1i1.1009 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 61-66 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1009/781 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1010 2022-07-13T07:08:07Z analis:ART

Pengaruh Perasan Daun Pandan Wangi (Pandanus amaryllifolius Roxb) Terhadap Shigella dysentriae Ariana, Diah  ABSTRACTDysentriae Shigella is a bacterial species of shigella that occurs in tropical countries, these bacteria are pathogenic gut bacteria are commonly known to cause dysentery. Dysentery is an infection that causes sores that cause ulcers limited dicolon characterized by symptoms known as dysentery syndrome. In user of trimatoprim sulfametaksazol can cause or accelerate the onset of megaloblastosis, leocopenia, or trombostopenia. In regular use, this combination appears to show little toxicity, approximately 75% of these adverse effects involving the skin. ‘Pandan wangi’ (pandanus amaryllifolius roxb) is one of the plants that can be used as a natural antibiotic against shigella dysentriae infection because the content of fragrant pandan leaves include flavonoids, alkaloids, saponins and tannins.This type of research is experimental, while the goal is to determine the effect  of ‘pandan wangi’ leaf (pandanus amaryllifolius roxb) juice on shigella dysentriae. Consisting of 7 concentrations and four repetitions. Using dilution method by observing the growth of bacteria shigella dysentriae on ‘pandan wangi’ leaf juice that is characterized by the colonies grown on media Mac Conkey (MC).Data analysis uses Anova with an error or a = 5%. The advanced test uses Tukkey test, Honestly Significant Difference (HSD). After doing the research, the optimal concentration of juice of ‘pandan wangi’ (pandanus amaryllifolius roxb) leaves can inhibit shigella dysentriae is 25% and can kill shigella dysentriae from concentration of 50%. The final conclusion is that there is the effect of ‘pandan wangi’ (pandanus amaryllifolius roxb) leaf juice on shigella dysentriae. Keywords: Pandanus amaryllifolius Roxb leaves, shigella dysentriae. Universitas Muhammadiyah Surabaya 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1010 10.30651/jmlt.v1i1.1010 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 67-72 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1010/800 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1011 2022-07-13T07:08:33Z analis:ART

Efektivitas Perasan Daun Bunga Sepatu (Hibiscus rosa-sinensis L) Terhadap Pertumbuhan Staphylococcus aureus Sispitasari, Yeti Eka Resistensi antibiotik merupakan masalah yang dihadapi oleh tenaga medis di seluruh dunia. Menurut WHO angka kematian akibat resistensi antibiotik sampai tahun 2014 sebesar 700.000 per tahun. Salah satu faktor pemicu meningkatnya kasus yakni penggunaan antibiotik yang tidak bijak pada manusia. Diperlukan alternatif antibiotik untuk mengendalikan infeksi bakteri, yang murah dan aman dengan menggunakan bahan alami berasal dari tanaman. Salah satu alternatifnya adalah daun bunga sepatu (Hibiscus rosa-sinensis L). Dalam Daun bunga sepatu terkandung senyawa antimikroba yaitu: taraxeryl acetat, flavonoid, polifenol, dan saponin. Tujuan penelitian ini adalah untuk mengetahui efektivitas perasan daun bunga sepatu (Hibiscus rosa-sinensis L) terhadap pertumbuhan Staphylococcus aureus.Jenis penelitian bersifat eksperimental laboratorium dengan post-test design group with control secara in vitro. Metode yang digunakan adalah metode difusi Ring dengan kosentrasi bahan uji yang digunakan 100%, 75%, 50%, 25%, dan kontrol negatif serta positif (tetrasiklin). Populasi penelitian adalah Staphylococcus aureus ATCC 25923 berasal dari Balai Besar Laboratorium Kesehatan Surabaya. Uji statistik yang digunakan adalah annova dengan syarat data harus berdistribusi normal dan homogen dan dilanjutkan ke uji tukey HSD.Hasil analisis data uji annova menunjukkan bahwa perasan daun kembang sepatu (Hibiscus rosa-sinensis L) efektif menghambat pertumbuhan Staphylococcus aureus dengan (nilai ρ<0,05). Diameter zona hambat perasan terbesar pada kosentrasi 100% yakni 26,6 mm dan terkecil pada 25% yakni 7,6 mm.Berdasarkan hasil penelitian daun bunga sepatu (Hibiscus rosa-sinensis L) belum bisa dianggap sebagai alternatif untuk penanggulangan penggunaan antibiotik kimia dikarenakan kontrol pembanding yang gunakan merupakan golongan antibiotik generasi pertama. Sehingga perlu diadakan penelitian dengan menggunakan kontrol pembanding antibiotik generasi terbaru. Kata kunci : Daun Bunga sepatu, Staphylococcus aureus  Universitas Muhammadiyah Surabaya 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1011 10.30651/jmlt.v1i1.1011 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 73-77 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1011/801 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1012 2022-07-13T07:08:55Z analis:ART

Efektivitas Perasan Daun Keji Beling (Sericocalyx crispus Linn) Dalam Menghambat Pertumbuhan Staphylococcus aureus Artanti, Dita Indonesia with the title gemah ripah loh jinawi is never separated from the wealth of its natural resources, especially on the type of flora. Indonesia is rich in the types of medicinal plants one of which is a Keji beling plant. The Keji beling plants by the community are used as anti diabetic, diuretic, antisipilis, antioxidant, antimicrobial, and laksatif. A research has been conducted to determine the effectiveness of the vermicelli leaves (Sericocalyx crispus linn) in inhibiting the growth of Staphylococcus aureus. This study was experimental laboratory consisting of 24 samples with 6 treatments with 4 replications. The concentration of the vermicelli leaves is 0%, 10%, 25%, 50%, 75%, and 100%. The method used in this research is dilution method. Data were analyzed by ANOVA analysis at 5% error rate (α = 0,05). The follow-up test uses Tukey's Honest Significant Difference (Tukey's HSD) test. The results showed that the vermicelli leaves are effective in inhibiting the growth of Staphylococcus aureus. The concentration of the best Keji beling leaves that can inhibit the growth of S. aureus is at 100% concentration because only 19 colonies grow. While the minimum inhibitory power is obtained at a concentration of 75%. Key word :  Keji beling leaves, Staphylococcus aureus Universitas Muhammadiyah Surabaya 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1012 10.30651/jmlt.v1i1.1012 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 78-83 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1012/802 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1013 2022-07-13T07:10:25Z analis:ART

Bioaktifitas Bunga Sepatu (Hibiscus rosa-sinensis L.) Terhadap Kadar Kolesterol Darah Mencit (Mus musculus) Yang Mengalami Hiperkolesterolemia Dengan Diet Tinggi Lemak Samsudin, Rinza Rahmawati  ABSTRAK Tujuan penelitian ini adalah untuk mengetahui pengaruh bioaktifitas ekstrak bunga sepatu (Hibiscus rosa-sinensis l.) terhadap  kadar kolesterol darah mencit (Mus musculus) yang mengalami hiperkolesterolemia dengan diet tinggi lemak. Penelitian ini merupakan penelitian eksperimental murni, dengan menggunakan rancangan randomized post-test only control group design. Bunga sepatu mengandung pigmen antosianin yang termasuk golongan flavonoid yang berperan sebagai antioksidan. Sebanyak dua puluh delapan mencit dibagi menjadi empat kelompok (K1, K2, K3 dan K4). Kelompok K1 adalah kelompok yang mendapat perlakukan pakan tinggi lemak dan aquadest setelah aklimatasi. Kelompok K2, K3, dan K4 adalah kelompok  terapi yang mendapat perlakukan pakan tinggi lemak, ekstrak bunga septum sebanyak 1 ml dan aquadest setelah aklimatasi. Pemberian pakan tinggi lemak berlangsung selama 28 hari. Selama penelitian berlangsung penimbangan berat badan tikus dilakukan sebanyak lima kali. Hasil penelitian menunjukan bahwa pemberian 1 ml ekstrak bunga sepatu selama dua puluh delapan hari dapat menurunkan kadar kolesterol mencit dimana nilai p<0.05. Kata kunci: ekstrak bunga sepatu, kadar kolesterol, diet tinggi lemak Universitas Muhammadiyah Surabaya 2017-12-22 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1013 10.30651/jmlt.v1i1.1013 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 84-89 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1013/803 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1015 2022-07-13T06:48:39Z analis:ART

Pengaruh Lama Perendaman Dengan Perasan Jeruk Lemon dan Garam Dapur Terhadap Kadar Protein Tahu Kartikorini, Nastiti  abstractTofu is one of the healthy foods because of the relatively high protein content. In know that stored at room temperature will only last for 1-2 days. Therefore, many sellers know who gives the formalin to preserve know. Whereas the use of formaldehyde is very harmful for health. One of the efforts to secure the preservation know that the provision of 10% lemon juice and salt to 4% who will preserve up to 6 days at room temperature. Just know that the old storage can cause denaturation of proteins which can result in decreased levels of protein out. This study aims to investigate the influence of soaking in lemon juice and salt to the protein content out. The protein content was obtained through laboratory know the Micro-Kjeldahl method. The sample was 8 were divided into 4 groups: control, know by soaking for 1 day, 3 days and 6 days. To determine the effect of soaking the protein content out statistical analysis used ANOVA and continued to LSD. The results of the data analysis ANOVA test showed that there was no effect of soaking time with the lemon juice and salt to the protein content of tofu (p values> 0.05). Average protein content know that soaked for 1 day 13.24%, 12.78% and 3 days 6 days 12.34%. So it can be concluded that aging out with lemon juice and salt has no effect on protein levels out. Therefore preservation know this way is relatively safe and does not reduce the levels of proteins out.Keyword : tofu, lemon, protein level Universitas Muhammadiyah Surabaya 2017-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1015 10.30651/jmlt.v1i1.1015 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 1-8 2614-2805 2597-3681 10.30651/jmlt.v1i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1015/758 Hak Cipta (c) 2017 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1483 2022-07-13T09:36:26Z analis:ART

Perbedaan Pertumbuhan Bakteri Shigella dysentriae Pada Berbagai Konsentrasi Perasan Kulit Apel Manalagi (Malus sylvestris Mill) Secara In Vitro Artanti, Dita Apple manalagi is one type of apple that is consumed by many Indonesian people, because it tastes sweet, tasty, easy to get and the price is quite affordable. The Manalagi apple rind contains some phytochemical derivatives of polyphenols that have antibacterial effects. Shigella dysentriae is a bacillus bacteria that causes acute diarrhea (dysentery) in most people who lack hygiene. One alternative to prevent the disease is to use natural ingredients as an antibacterial. The aim of this research is to know the different growth of S. dysentriae bacteria in various concentrations of Manalagi apple rind (Malus sylvestris Mill.) In vitro. The type of this study was experimental consisting of 7 treatments with 4 repetitions. The sample consisted of 7 concentrations of manalagi apple rind (100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 0% (control) .The data analysis used ANOVA test with 5% 0.05). The results showed that the growth of S.dysentriae bacteria was only at 0% concentration; 3.125%; 6.25%; 12.5%; and 25%. While at concentrations of 50% and 100% there was no growth of S.dysentriae bacterial colonies. The conclusion of this study proves that Manalagi apple rind has antibacterial power against S.dysentriae bacteria.Keywords: Shigella dysentriae, Manalagi Apple Rind (Malus sylvestris Mill). Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1483 10.30651/jmlt.v1i2.1483 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 90-102 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1483/1282 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1484 2022-07-13T09:36:26Z analis:ART

Perbandingan Pemeriksaan Leukosit Urine Segar Dengan Setelah 2 Jam Di Suhu Kamar Purwaningsih, Nur Vita ABSTRACTBackground: Urinalysis is a parameter often requested by clinicians. The urinalysis parameters consist of macroscopic, microscopic or sedimental examination and urine chemistry examination. Urine examination is very important, especially in making the diagnosis. Procrastination delay results in errors in diagnosis and administration of drugs that lead to adverse outcomes of patients, analysis should be performed no later than 4 hours after sampling. Urine has a stability at room temperature ie for 1 hour, if urine is silenced long then the bacteria will multiply, so it can decompose NH3 (ammonia) which is alkaline. Under alkaline conditions, the pH in the urine will increase. This may affect the sediment component in the urine to rapid lysis so that the amount will be reduced General purpose of this study to determine the difference results of examination of fresh urine leukocytes compared after 2 hours at room temperature. Method: descriptive observasional with number of sample counted 20 sample. Result: Based on the test result, the positive percentage value in fresh urine is 100% and the positive percentage value in urine after 2 hours is 70%. In the Wilcoxon statistical test obtained p-value <0.001 (> 0.05). Conclusion: Based on the research that has been done then it can be concluded that there are differences in the results of fresh urine leukosit better than urine after 2 hours at room temperature.Keywords: Leukocyte urine, Fresh urine, delay.  Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1484 10.30651/jmlt.v1i2.1484 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 103-109 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1484/1287 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1487 2022-07-13T09:36:26Z analis:ART

Hubungan Kadar TSH Terhadap Kadar FT4 Pada Pasien Tiroid Di Bangkalan Maulidiyanti, Ellies Tunjung Sari AbstractThyroid disorder disease ranks second most in the list of metabolic diseases after diabetes mellitus (DM). Thyroid disease or Thyroid abnormalities is a condition of abnormalities in a person due to the disorder of the thyroid gland. TSH is a primary factor that controls thyroid cell growth and synthesis also thyroid hormone secretion. TSH secretion is stimulated by low levels of T3 and T4 and by the hormone TRH (Thyroid Releasing Hormone) the hypothalamus is inhibited by elevated levels of T3 and T4. T3 and T4 are circulating in the plasma, most of which are bound with proteins, Thyroid Binding Globulin (TBG) and a small part in free form that are Free Triiodotironine (FT3) and Free Thyroxine (FT4). The free hormone (FT3 and FT4) is an actively metabolic fraction which is known to be quantitative. Currently the measurement of free hormone levels should be part of a complete examination of the status of the thyroid gland. The purpose analyzes the levels of TSH, FT4 and proves the relationship between the two in patients with thyroid disorders. This examination by using ELISA method is with EIA competitive principles and sandwiches. The samples are serum of patients with thyroid disorders at RSUD Bangkalan and Farmalab Laboratory from October to November 2017.The 30 samples obtaines an average result of TSH level of 1.9 mIU / L and an average FT4 level of 2.8 ng / dl. Next test Kolmogorov-Smirnov and Spearrman's test results obtained if the variable one value rose (high) then the other variable down (low). It can be concluded that there is an opposite relationship between TSH levels and FT4 levels, if TSH levels increase, the FT4 level decreases, and so it is also in contrast Keywords: TSH, FT4, thyroid disorders Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1487 10.30651/jmlt.v1i2.1487 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 110-119 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1487/1288 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1495 2022-07-13T09:36:26Z analis:ART

Respon Inflamasi Pada Perokok Pasif Di Kecamatan Pahandut Kota Palangka Raya Ditinjau Dari Jumlah Leukosit Dan Jenis Leukosit ardina, rinny Proporsi perokok pasif di Indonesia mencapai 40,5 persen dan 78,4 persen perokok pasif banyak terpapar asap rokok di dalam rumah.Setiap hembusan asap rokok mengandung 1015 radikal bebas oksidatif dan nikotinpenyebab leukositosis di dalam darah sebagai bentuk respon inflamasi.Jenis leukosit penunjuk inflamasi akibat asap rokok diawali neutrofilia, neutropenia, limfositosis, limfopenia dan monositosis sebagai bentuk respon inflamasi. Analisis deskriptif dengan rancangan cross sectional digunakan untuk memberikan gambaran respon inflamasi ditinjau dari jumlah leukosit dan jenis leukosit pada perokok pasif yang tinggal di kecamatan Pahandut kota Palangka Raya. Sampel yang diperoleh sebanyak 30 orang diambil dengan teknik Purposive Sampling dimulai dengan observasi, pembagian lembar inform consent, wawancara, pengisian kuesioner, pengambilan sampel darah dan pemeriksaan laboratorium menggunakan hematology analyzer dan apusan darah tepi. Data yang diperoleh dimuat dalam bentuk tabel dan dianalisis dan dideskripsikan dalam bentuk persentase (%). Pada 5 orang (17%) terjadi leukositosis, 2 orang (6%) neutropenia, 1 orang (3%) neutrofilia, 1 orang (3%) limfopenia, dan 6 orang (20%) limfositosis. Leukositosis menunjukkan penanda adanya perubahan imun sistemik, dimana selanjutnya terjadi rekrutmen sel inflamasi diantaranya neutrofil dan limfosit. Radikal bebas dan nikotin memicu inflamasi ditandai dengan neutrofilia dengan limfopenia relatif pada awal inflamasi dan selanjutnya berkembang menjadi limfositosis dengan neutropenia relatif.Proportion of passive smokers in Indonesia reached 40,5% and 78,4% of them were exposure of cigarette smoke in the house. Each puff of cigarette smoke contains 1015 of oxidative free radicals and nicotine that causes leukocytosis in the blood where it found as an inflammatory response. Either free radicals or nicotine can also cause of neutrophilia, neutropenia, lymphocytosis, lymphopenia, and monocytosis. This study aimed to overview of the inflammatory response in terms of white blood cell count and differential counting of leukocytes in passive smokers (housewives) who live in the district of Pahandut, Palangka Raya. Descriptive analysis with cross sectional design was used in this study. Samples obtained by 30 people were taken with Purposive Sampling technique. Procedure of collecting data starts from observation, distribution of informed consent, interview, answered of questionnaire, blood sampling and laboratory examination using hematology analyzer for measured of white blood cell count and peripheral blood smear for measured of differential counting of leukocytes. The data obtained were analyzed and described in percentage (%). This study found 5 people (17%) of leukocytosis, 2 people (6%) of neutropenia, 1 person (3%) of neutrophilia, 1 person (3%) of lymphopenia, and 6 people (20%) of lymphocytosis. Leukocytosis occurs because of the mechanism of leukocytes recruitment into inflammatory tissues and the types of leukocyte that increased are neutrophils and lymphocytes. Free radicals and nicotine play a major role in triggering inflammation. Acute inflammation cause of exposure of cigarette smoke characterized by neutrophilia and relative lymphopenia in the blood and it will be develop into chronic inflammation that characterized by lymphocytosis and relative neutropenia. Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1495 10.30651/jmlt.v1i2.1495 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 120-130 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1495/1283 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1496 2022-07-13T09:36:26Z analis:ART

Keanekaragaman Genetik Nyamuk Vektor Filariasis Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) di Kota dan Kabupaten Pekalongan Dengan Metode PCR-RAPD Arimurti, Anindita Riesti Retno Mosquito Culex quinquefasciatus is a vector of nematode worms, namely Wuchereria bancrofti which is the cause of filariasis in tropical and subtropical countries. Distributed of Cx. quinquefasciatus is widely in Indonesia with differences the geographical, resulting in the adaptation to the environment and may  results in a high variation, both phenotypic (morphology) and genotypic (genetic) variation. This study aims was to determine the genetic diversity of mosquitoes Cx.quinquefasciatus as vector filariasis in Pekalongan City and Regent. Genetic characterization performed by PCR-RAPD using three primers, ie OPA-11, OPA-12, and OPA-15. Data were analyzed by using UPGMA algorithm and Simple Matching Coefficient and presented as dendrogram. The results showed a high genetic diversity with the polymorphisms up to 100%.  Keywords: Culex quinquefasciatus, vector, filariasis, PCR-RAPD  Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1496 10.30651/jmlt.v1i2.1496 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 131-150 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1496/1284 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1499 2022-07-13T09:36:26Z analis:ART

Gambaran Histologi Ginjal Tikus Wistar Yang Terpapar MSG Setelah Perlakuan Diberikan Jus Tomat Dan Diberhentikan Perlakuan Saja Sispitasari, Yeti Eka MSG causes many side effects on the body, but MSG has long been used as a food flavor that can bring tastes (umami) and play a role in strengthening the taste. The chemical structure of MSG is no different from Glutamic Acid (glutamate), it is one of the 20 amino acids that make up proteins in the body. So the use of MSG needs to be discontinued to prevent kidney damage due to continued consumption of MSG, two research were conducted to determine the effect of discharging of MSG consumption by being given tomato juice (1) and discharged without treatment (2), the second study was seen from the histology picture of rats wistar mouse.Methods were experimental with post test only control group design. The research (1) used 15 wistar rats divided into three groups: group I without treatment (pellet AD II and drinking water), group II was given MSG for 14 days and group III was given tomato juice and MSG for 14 days. The study (2) used 27 rats divided by 9 groups. The sample is selected by simple random sampling method. The mice were then gradually turned off on the 29th, 43rd and 57th days. There was no significant difference in mean number of normal and damaged proximal tubules in all treatment groups. The renal histologic features in the treatment group I (MSG) and the treatment group II (MSG and tomato juice) showed normal glomeruli, tubular epithelial tubules, and lumen tubular narrowing whereas in mice discharged without treatment there was no apparent difference between the two MSG Giving groups damage to proximal tubules and renal corpusculum and regeneration after 14 days of discontinuation of MSG. Conclusions from these two experiments occurred damage to the renal tubules Keywords: MSG, Kidney, Wistar Rat Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1499 10.30651/jmlt.v1i2.1499 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 151-158 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1499/1286 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1500 2022-07-13T09:36:26Z analis:ART

Variasi Kandungan Merkuri (Hg) Pada Berbagai Macam Bedak Whitening Yang Dijual Pasar Blauran Surabaya Kartikorini, Nastiti Mercury is widely used in cosmetics to remove black spots on the face. Mercury in the smallest amount can be a toxic such as skin discoloration, black spots, allergies and irritation. High doses of mercury can cause permanent damage to the brain, kidneys and impaired development of the fetus,distruction skin surface.The purpose of this study was to analyze mercury levels in various brands of whitening powder sold in Blauran  market, Surabaya. The samples were 22 types of whitening powder. The samples were analyzed by Atomic Absorption Spectrophotometer (AAS).According to the results of research on mercury content (Hg) of 22 whitening powder, it is known that 4 whitening powder contain mercury (Hg), and have mercury (Hg) which is vary from the highest level (0.016 ppm) and the lowest level (0,008 ppm). However, the mercury content (Hg) is still below the maximum limit set. Keywords: Whitening Powder, Mercury, Atomic Absorption Spectrophotometer (AAS) Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1500 10.30651/jmlt.v1i2.1500 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 159-165 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1500/1291 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1501 2022-07-13T09:36:26Z analis:ART

Perbedaan Zona Hambat Terhadap Jamur Malassezia Furfur Antara Pemberian Ekstrak Umbi Bawang Putih (Allium Sativum Linn) Dengan Ekstrak Kulit Umbi Bawang Putih (Allium Sativum Linn) Ariana, Diah Tinea versicolor or pitiriasis versicolor is a skin disorder caused by Malassezia furfur infection which is a fungal infection on the skin of the chronic surface. Clinical features is in the form of fine spots that colored white to brown black, this infection is also called a skin fungus with blotches. Treatment of Tinea versicolor can use chemical or traditional medicine. Chemical treatments such as creams or ointments cause sticky taste, while one of the traditional medicine is the bulb and skin of the garlic bulb (Allium sativum Linn) containing allicin substances that are efficacious as an anti-fungal.               This research is an experimental research with the aim to know the difference of inhibition zone to Malessezia furfur fungus. There are 3 treatments with 9 repetitions. The extraction method used is the soxhlet method. Anti-fungal activity test was done by using rating method, Saboroud Dextrose Agar (SDA) media which has been inoculated with suspension of Malassezia furfur with concentration 1.5x108 CFU/ml (Mc Farland standard).               Result of data analysis obtained by ANOVA p = 0,000 < α = 0,05, that there is difference of inhibition zone from treatment of garlic bulb extract and skin of garlic bulb (Allium sativum Linn). It is indicated that there is effect of giving garlic bulb extract (Allium sativum Linn) and garlic bulb skin (Allium sativum Linn) to Malassezia furfur fungus. Further tests were performed to compare mean treatment i.e. HSD test. It was obtained sig (p) 0,016 > α 0,05 which means bulb extract and garlic bulb skin (Allium sativum Linn) have different effect. Keywords: Tinea versicolor, Malassezia furfur, garlic bulb. Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1501 10.30651/jmlt.v1i2.1501 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 166-176 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1501/1290 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1541 2022-07-13T09:36:26Z analis:ART

Bioaktif Belimbing Wuluh (Averhoa bilimbi (Linn.)) Terhadap Kadar Formalin Dalam Tahu Samsudin, Rinza Rahmawati This study aims to determine the influence of the juice of belimbing wuluh to the content of formalin in tofu and know the concentration of belimbing wuluh feeling is most effective in reducing the content of formalin in tofu. This study used a complete randomized design. The sample of this study was 30 tofu containing  formalin which was divided into 6 groups. The weight of each sample is 15 grams.The data of the research were analyzed by using anova test with 95% confidence level, then Duncan test with 95% confidence level. From the result of this research can be concluded that there is influence to formalin content in tofu. The content of formaldehyde in the highest to the lowest yields was found in soaking the belimbing wuluh with the concentrations is the group without treatment (8269.40 mg / Kg), 0% (1434.20 mg / Kg), 100% (762.00mg / Kg), 25% (573.80 mg / Kg), 75% (442.20 mg / kg), and 50% (162.00 mg / kg). The highest decrease in formaldehyde content in tofu by 98.40% occurred in the group K3 with the most effective 50% concentration to decrease the formalin content in tofu. Keyword : belimbing wuluh, tofu, formalin Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1541 10.30651/jmlt.v1i2.1541 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 177-186 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1541/1292 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1543 2022-07-13T09:36:26Z analis:ART

Efek Anti Oksidan Bawang Putih terhadap Penurunan Bilangan Peroksida Minyak Jelantah Mardiyah, Siti Used cooking oil is leftover cooking oil from food frying processes. The use of cooking oil which is repeated with a high temperature and a long time can cause the oil to oxidation and hydrolysis, resulting in increased levels of peroxide. High peroxide value can pose a health hazard. Allisin is antioxidants contained in garlic. Allisin are primary antioxidants which act as free radical scavengers that can slow oxidation on used cooking oil. The purpose of this study was to determine the effect of garlic on decreased levels of peroxide in used cooking oil. This type of research is experimental by the number of samples in each treatment as many as 4 samples. Formulation of the problem of this study is whether there is the effect of adding garlic to decreased levels of peroxide on used cooking oil.From the results of the average levels of peroxide in used cooking oil that has been saved within 3 days is 26.0482 control mEq, 10 mEq% 23.65098, 22.71355 mEq 20%, 30% mEq 18.65023, 17.92128 mEq 40%, 50% 16.52508 mEq. Statistical analysis with ANOVA test shows the effect of adding garlic to decreased levels of peroxide in used cooking oil with p value (sig) = 0.000 which is smaller than 0.05. The results of Tukey HSD test showed the addition of garlic 30% is effective to reduce levels of peroxide on used cooking oil.Therefore, the addition of garlic may inhibit the process of rancidity and lower levels of peroxide in used cooking oil.KEYWORDS: garlic, cooking oil, peroxide value Universitas Muhammadiyah Surabaya 2018-05-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1543 10.30651/jmlt.v1i2.1543 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 1 No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist; 187-199 2614-2805 2597-3681 10.30651/jmlt.v1i2 ind https://journal.um-surabaya.ac.id/analis/article/view/1543/1293 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1804 2022-07-13T09:51:20Z analis:ART

Gambaran Bakteri Kontaminan pada Sikat Gigi Nursidika, Perdina Naully, Patricia Gita Lestari, Linda Ayu Abstract: DESCRIPTION OF BACTERIAL CONTAMINTANT IN TOOTHBRUSH. Brushing activity is an effort to keep oral hygiene from pathogenic microorganisms that can cause infection. Oral infection has been an increasingly common risk factor for systemic disease. Oral infections, especially periodontitis, can affect travel and pathogenesis of a number of systemic diseases, such as cardiovascular disease, bacterial pneumonia, diabetes mellitus, and low birth weight. Keeping your teeth and mouth clean is not enough just brushing your teeth, but replace the toothbrush for a maximum of three months. This study aims to identify the contaminant bacteria found on toothbrushes that have been used for more than 3 months. The method of identification by planting the isolation result of 20 toothbrushes has been used for more than three months on selective media ie blood agar (AD), McConkey Agar (MCA), Eosin Methylen Blue (EMB), Manitol Salt Agar (MSA) . The results showed that all samples of toothbrush were contaminated with Streptococcus and Staphylococcus, 16 samples were Klebsiella (80%), four samples (20%) were Proteus, and one sample (5%) was contaminated with Escherichia. All of the toothbrush samples showed to have been contaminated by bacteria. Bacterial contamination of the toothbrush can be caused by the storage of a toothbrush that is too close to the toilet and toilet, possibly exposed through aerosols containing intestinal bacteria. This toothbrush contamination plays an important role in both oral and systemic diseases, including septicemia and gastrointestinal, respiratory cardiovascularmy, and kidney problems. Abstrak: GAMBARAN BAKTERI KONTAMINAN PADA SIKAT GIGI. Kegiatan menyikat gigi merupakan upaya untuk menjaga kebersihan gigi dan mulut dari mikroorganisme pathogen yang dapat menyebabkan infeksi. Infeksi mulut telah menjadi faktor risiko yang semakin umum untuk penyakit sistemik. Infeksi mulut, terutama periodontitis, dapat mempengaruhi patogenesis dari sejumlah penyakit sistemik, seperti penyakit kardiovaskular, pneumonia bakteri, diabetes mellitus, dan berat badan lahir rendah. Menjaga kebersihan gigi dan mulut tidak cukup hanya menyikat gigi saja, melainkan mengganti sikat gigi maksimal tiga bulan sekali. Penelitian ini bertujuan untuk mengidentifikasi bakteri kontaminan yang terdapat pada pada sikat gigi yang telah digunakan selama lebih dari 3 bulan. Metode identifikasi dengan melakukan penanaman hasil isolasi 20 sikat gigi yang telah digunakan selama lebih dari tiga bulan pada media selektif yaitu agar darah (AD), McConkey Agar (MCA), Eosin Methylen Blue (EMB), Manitol Salt Agar (MSA) dengan metode gores.  Hasil penelitian menunjukkan semua sampel sikat gigi terkontaminasi Streptococcus dan Staphylococcus, 16 sampel terdapat Klebsiella (80%), pada empat sampel (20%) terdapat Proteus, dan satu sampel (5%) terkontaminasi Escherichia. Semua sampel sikat gigi menunjukkan telah terkontaminasi oleh bakteri. Kontaminasi bakteri pada sikat gigi ini dapat disebabkan oleh penyimpanan sikat gigi yang terlalu dekat dengan jamban, sehingga kemungkinan terpapar melalui aerosol yang mengandung bakteri usus. Kontaminasi sikat gigi ini memegang peranan penting pada penyakit oral maupun sistemik, termasuk septikemia dan gastrointestinal, kardiovaskularm pernapasan, dan masalah ginjal. Universitas Muhammadiyah Surabaya 2018-11-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1804 10.30651/jmlt.v2i1.1804 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 34-50 2614-2805 2597-3681 10.30651/jmlt.v2i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1804/1779 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1834 2022-07-13T09:50:37Z analis:ART

Gambaran Anti-Plasmodium sp. Pada Anggota Tentara Nasional Indonesia Angkatan Darat Di Kota Cimahi Naully, Patricia Gita Khairinisa, Gina Saputri, Dewi Ratih Malaria is a disease caused by Plasmodium sp. The presence of such parasites in body can be characterized by the presence of anti-Plasmodium sp. antibodies. Malaria cases are still prevalent in Indonesia and generally occur in people who travel to endemic areas. One of the jobs that obliges its members to serve to various regions, including endemic areas is the Indonesian Army (TNI AD). Many Army personnel residing in Cimahi City. The purpose of this study is to determine the overview of malaria in members of the Army in Cimahi City. This study used a sample of 30 TNI AD who are residing in Cimahi City and have been assigned to various regions in Indonesia. Venipucture is only performed on respondents who have signed informed consent and filled out the interview form. Malaria screening is performed using immunochromatography that can detect anti-P. vivax and anti-P. falciparum antibodies, with a high degree of sensitivity and specificity. Of the 30 people, there were 4 people (13.3%) who had been infected with malaria and 2 (6.7%) of whom had experienced mixed malaria. All respondents who tested positive for malaria claimed to have been assigned to malaria endemic areas such as Papua and Maluku for several years. Universitas Muhammadiyah Surabaya 2018-11-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1834 10.30651/jmlt.v2i1.1834 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 18-28 2614-2805 2597-3681 10.30651/jmlt.v2i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1834/1629 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1921 2022-07-13T09:50:02Z analis:ART

Uji Efektivitas Formula Pedikulosida Berbahan Aktif Minyak Atsiri Terhadap Mortalitas Kutu Kepala (Pediculus humanus capitis De Geer) Secara In Vitro Arrizqiyani, Tanendri Pediculosis is a disease caused by Pediculus humanus capitis de Geer or often called head lice which is an obligate ectoparasite in the human head. The prevalence of pediculosis is quite high and there are reports of resistance and negative side effects regarding the use of synthetic pediculosides trigger research and development of vegetable pediculosides. This study aims to examine the effectiveness of the formula of pediculoside with active ingredients of essential oils on head lice mortality in vitro. The study was conducted by testing 3 formulas with variations in the composition of essential oils made from cassava oil, fennel oil, lemon oil, water and vegetable glycerine. Comparison of the ingredients in formula A is 10%, 10%, 5%, 50% and 20%; formula B is 5%, 15%, 5%, 50% and 20%; while formula C is 15%, 5%, 5%, 50% and 20%. The test method is an experiment with a completely randomized design. The test is done by placing 10 adult head lice on filter paper which has been saturated with a formula made in a petri dish. Mortality of head lice is observed based on time. Test results data will be compared with negative controls and positive controls in the form of testing using permethrin-active pediculoside drugs. The results showed that the pediculoside formula A, B and C had a high mortality rate which caused 100% of the deaths of head lice tested in less than 5 minutes. Statistically there is a significance to the results of testing formulas A, B, and C when compared to controls. Based on the results of multiple statistical statistics, it was found that formula C was the most effective as a pediculoside formula Universitas Muhammadiyah Surabaya 2018-11-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1921 10.30651/jmlt.v2i1.1921 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 1-11 2614-2805 2597-3681 10.30651/jmlt.v2i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1921/1627 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/1999 2022-07-13T05:20:39Z analis:ART

Perbandingan Hasil Pemeriksaan Glukosa Darah Dengan Menggunakan Serum Dan Plasma Natrium Fluorida (NaF) Di Laboratorium Klinik Medika Jayapura Sinaga, Herlando Irianti, Cuti The purpose of this research was to compare the results of examination of blood glucose levels using serum and plasma Sodium Fluoride (NaF). The population used in this study were all patients who came to check blood glucose while at the Medika Clinic Laboratory. The number of samples in this research was 67 (total population). The method used in examination of blood glucose is the enzymatic method of Glucose Oxidase (GOD - PAP). This research was conducted for 1 month, from April 1 to April 30, 2017. To compare the values between serum and plasma sodium fluoride (NaF) specimens, it was analyzed statistically using the Social Science Stastistial Program (SPSS) for windows version 21 with the test method Mann Whitney and obtained a mean of 154.49 mg/dl in serum specimens and 145.36 mg/dl in plasma specimens of sodium fluoride (NaF). The results of statistical tests of blood glucose levels with serum and plasma NaF specimens showed no significant difference and obtained a significance value of 0.196 which means a probability >0.05. The conclusion of this research is that there is no significant difference between the examination of blood glucose using serum specimens and plasma Sodium Fluoride (NaF).Keywords : Blood Glucose, Serum, Sodium fluorida (NaF) Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/1999 10.30651/jmlt.v3i1.1999 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 69-76 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/1999/2857 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/2211 2022-07-13T09:50:20Z analis:ART

Efek Pemberian Gentamisin Secara Oral Terhadap Kadar Asam Urat, Ureum, dan Kreatinin Tikus Wistar Ardiansyah, Syahrul ABSTRACTGentamicin is an aminoglycoside class of antibiotics which at high doses can cause damage to the kidneys. This research was to determine the levels of BUN, creatinine and amino acids after gentamicin induction. This research is experimental with gentamicin test material given to male rats as much as 0.3 ml/day for 7 and 10 days. Blood serum levels were then measured for BUN, creatinine and amino acids using a photometer microlab 300. The results showed that after doing treatment for 7 days the average value of BUN levels increase from 21.7 to 36.3 mg/dl, creatinine increase from 0, 88 to 1.9 mg/dl, uric acid increase from 1.54 to 4.29 mg/dl. It was concluded that giving of gentamicin as much as 0.3 ml/day for 7 and 10 days increased BUN levels, creatinine and uric acid exceeded the normal limit. Keywords : Gentamicin, urea, creatinine, uric acid, rat. Universitas Muhammadiyah Surabaya 2018-11-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/2211 10.30651/jmlt.v2i1.2211 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 11-17 2614-2805 2597-3681 10.30651/jmlt.v2i1 ind https://journal.um-surabaya.ac.id/analis/article/view/2211/1628 Hak Cipta (c) 2018 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/2231 2022-07-13T09:51:01Z analis:ART

Analisa Asam Salisilat Pada Perlengkapan Persedian Bermerk Bayi Di Pasar Blauran Surabaya Kartikorini, Nastiti Salicylic acid is a substance that is often added to skin care products for acne. In the regulation of the National Agency of Drug and Food Control No. Hk.03.1.23.08.11.07517 in 2011 concerning the technical requirements of cosmetics, salicylic acid levels are limited to 3% for rinse production and 2% for other production and are not used for children under 3 years. Using at high doses can cause the baby to bleed, mute and deaf. This is because the structure of the baby's skin is still thin, so it becomes susceptible to irritation and infection. The title of this research is Analysis of Salicylic Acid in the mains supplies barnded baby in the market blauran in Surabaya Region. The purpose identified salicylic acid content with qualitative tests on baby cosmetics circulating in Surabaya. The formulation of this problem is the presence or absence of Salicylic Acid in baby cosmetics. The type of research used descriptive, there were 26 baby cosmetics products in 4 types of products in the form of powder, soap, shampoo, and lotion which were conducted in the Laboratory of Muhammadiyah University of Surabaya in June 2018. Based on the results that found from 26 samples of baby cosmetics in the form of powder as much as 100% of soap, shampoo and lotions sold in Surabaya were not found for Salicylic Acid. So that all samples met the requirements based on the regulation of the National Agency of Drug and Food Control No.Hk.03.1.23.08.11.07517 in 2011 concerning the technical requirements of cosmetics materials, salicylic acid levels were limited to 3% not used for children under 3 years.           Keywords: Baby supplies, Salicylic Acid Universitas Muhammadiyah Surabaya 2018-11-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/2231 10.30651/jmlt.v2i1.2231 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 29-33 2614-2805 2597-3681 10.30651/jmlt.v2i1 ind https://journal.um-surabaya.ac.id/analis/article/view/2231/1778 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/2449 2022-07-13T04:25:08Z analis:ART

HUBUNGAN FREKUENSI MENGURAS TERHADAP PERTUMBUHAN JAMUR PADA AIR BAK TOILET TEMPAT WISATA DI WILAYAH KOTA KEDIRI Humairoh, Durroh Asmarani, Endrik Tempat wisata merupakan suatu tempat menarik yang dituju bersama rekan, teman, ataupun keluarga untuk berlibur. Setiap tempat wisata selalu dilengkapi toilet yang bersih demi kenyamanan pengunjung. Pada tempat wisata yang menyediakan toilet umum harus selalu dibersihkan dengan teratur supaya tidak terinfeksi oleh mikroorganisme yang dapat membahayakan kesehatan. Pada penelitian ini, dilakukan penelitian pada beberapa toilet tempat wisata di wilayah kota Kediri. Metode yang dilakukan pada penelitian ini dengan centrifugasi sebanyak 15 tempat wisata di kota Kediri. Hasil penelitian dengan uji korelasi spearman’s rho menunjukkan terdapat hubungan frekuensi menguras dengan jumlah jamur yang tumbuh pada air bak toilet dan hasil tersebut menunjukkan bahwa semakin lama masa frekuensi menguras bak toilet, maka semakin tinggi pertumbuhan jamur yang muncul di air bak toilet tersebut. Kata kunci: frekuensi menguras, pertumbuhan kapang, tempat wisata Universitas Muhammadiyah Surabaya 2019-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/2449 10.30651/jmlt.v2i2.2449 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 1-9 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/2449/2243 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/2581 2022-07-13T04:25:08Z analis:ART

Analisis Kadar Trigliserida dan HDL (High Density Lipoprotein) Pada Tenaga Farmasi di Apotek Indobat Denpasar Indrayani, A.A. Sagung Mas AbstractNight shift can cause health problems one of which is the body's circadian rhythm disturbance which can lead to increased triglyceride levels and decreased levels of HDL (High Density Lipoprotein). This study to find out differences in triglyceride and HDL levels in pharmacist before and after night shift in Apotek Indobat Denpasar. This study method is pre-experimental one group pretest-posttest design. Blood collection was carried out on 28 pharmacists before and after the night shift. Triglyceride levels were measured using the GPO-PAP (Glycerol Peroxidase Phosphate Acid) method and HDL measured using the CHOD-PAP (Cholesterol Oxidase Diaminase Peroxidase Aminoantipyrine) method with a chemistry analyzer. The average triglyceride levels before the night shift were 87.05 ± 0.53 mg/dL, and levels after the night shift were 105.77 ± 0.50 mg/dL with a percentage increase in triglyceride levels was 24.66%. The average HDL levels before the night shift were 59.84 ± 0.47 mg/dL, and the levels after the night shift were 48.61 ± 0.42 mg/dL with a percentage decrease in HDL levels was 27.24%. Based on the results of the study, the night shift can increase triglyceride levels and decrease HDL levels. Keywords: chemistry analyzer, CHOD-PAP, HDL, GPO-PAP, pharmacist, triglycerides. Universitas Muhammadiyah Surabaya 2019-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/2581 10.30651/jmlt.v2i2.2581 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 18-27 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/2581/2246 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/2584 2022-07-13T04:25:08Z analis:ART

EFEKTIVITAS BUNGA WIJAYA KUSUMA (Epiphyllum oxypetalum) TERHADAP PENURUNAN KADAR ASAM URAT TIKUS WISTAR yuan, rahayu artini ABSTRACT            The isolation of wijaya kusuma (Epiphyllum oxypetalum) flower was carried out in this study. Methanol was applied to maceration an  27 g sample of extract its was produced from 250 g of sample powder. In vivo test results, methanol extract of wijaya kusuma flower (Epiphyllum oxypetalum) dose of 100 mg / kg BB, 200 mg / kg BB, and 400 mg / kg BW can decreasing uric acid concentration. From this research, it was obtained that the best dose of extract of wijaya kusuma flower (Epiphyllum oxypetalum) applied to decrease uric acid was 400 mg/Kg BW with a decrease percentage of 63,50%. The probit test results obtained the value of ED50 methanol extract of wijaya kusuma flower (Epiphyllum oxypetalum) of 221 mg / Kg BB. In this study it can be concluded that soursop leaf extract (Annona muricata L.) can reduce uric acid levels in research mice. For future research, this method could be applied to human in decreasing uric acid concentration in blood.Keywords: kusuma wijaya flower (Epiphyllum oxypetalum), extraction, maceration, and uric acid. Universitas Muhammadiyah Surabaya 2019-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/2584 10.30651/jmlt.v2i2.2584 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 37-46 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/2584/2253 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/3090 2022-07-13T04:25:08Z analis:ART

Uji Toksisitas Akut Kulit Kentang (Solanum tuberosum L.) Terhadap Larva Artemia salina Leach Dengan Metode BSLT (Brine Shrimp Lethality Test) Kunsah, Baterun Tanggal Submit:28 Januari 2019 Tanggal Review:30 April 2019 Tanggal Publish Online:17 Mei 2019 Potatoes are one of the vegetables that contain substances that are important for the formation of body tissues, such as protein and fat. Potatoes also form red blood cells or hemoglobin (Ca, P and Fe). In Indonesia tend to process potatoes using only meat. The skin is removed, even though the nutritional content of the skin is five times greater than the meat. Potato peels that supply querestin, antioxidants and flavonoids that act as free radical acceptors. (Free radicals are reactive molecules that cause damage to the body that can lead to diseases such as heart disease and cancer) in the skin of potatoes found also effective antioxidants, chlorogenic acid. Potato skin is also proven to increase hemoglobin levels (Khomsan, A, 2009). However, that does not mean potato skin does not have adverse side effects, if the use is not appropriate. In order to use it optimally, you need to know enough information about the advantages and disadvantages and the possibility of abuse. However, the level of toxicity from potato skin is still unknown. Brine Shrimp Lethality Test (BSLT) is one of the oxicity test methods that is widely used in tracing bioactive compounds that are toxic from natural materials. This research is an experimental research with post test-only control group design approach. Treatment with giving potato skin juice to Artemia salina Leach larvae with the aim to determine the potential of plant biological activity based on the toxicity of secondary metabolites contained in it, and at the same time as the initial screening test of anticancer activity of chemical compounds in potato skin juice. The results showed the LC50 value was at a concentration of 0.5% to 1% and based on the results of SPSS 21 analysis with probit analysis obtained LC50 price data of 0.746 gr / ml (7460 μgr / ml), based on the results of research and data analysis it can be concluded that potato skin has an LC50 value at a concentration of 0.746 gr / ml (7460 µgr / ml). With an LC50 price of more than 1000 µg / ml according to the BST method the potato skin does not have the potential for toxicity so the skin of the potato has the potential as an alternative to increasing hemoglobin levels.Keywords: potato skin, toxicity, BSlT Universitas Muhammadiyah Surabaya 2019-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/3090 10.30651/jmlt.v2i2.3090 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 10-17 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/3090/2245 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/3380 2022-07-13T04:25:08Z analis:ART

Efektivitas Vitamin C Pada Daun Kelor Terhadap Bilangan Peroksida Dari Minyak Jelantah Kartikorini, Nastiti Used cooking oil is a cooking oil that has been used several times. Used cooking oil undergoes an oxidation reaction to produce high peroxide compounds. Phenolic compounds are primary antioxidants contained in Moringa leaves which slow the oxidation down in used cooking oil so that it can be stored longer. Formulation of the problem in this study was whether there was an inhibitory test on Moringa oleifera Leaves powder on the peroxide value in used cooking oil. The purpose of this study was to determine the inhibition of Moringa leaves powder to peroxide value on used cooking oil. This type of research was an experimental i.e. to determine whether there was the inhibition of Moringa leaves powder to the peroxide value in used cooking oil. The sample in this study was 5 samples from 6 fried traders, and repeated 5 times so that the total sample was 25 samples. Peroxide levels could be decreased due to the antioxidant content found in Moringa leaves. Antioxidants found in Moringa leaves are phenolic components. Phenolic components can inhibit free radicals and inhibit peroxide value to decrease. Moringa was suitable for areas where strong and long winds, drought occur simultaneously, and cause serious soil erosion. Nutrient content in deciduous leaves could fertilize and improve marginal soil quality (Dudi, 2015). Therefore, Moringa leaves not only have high antioxidants but also could be found easily in Surabaya. From the results of peroxide levels test, it was known that the average peroxide value in used cooking oil without Moringa leaves was equal to 23,9957 mEq and with Moringa leaves by different time variation i.e. 1 day (14,3182 mEq), 2 days (10,7987 mEq), 3 days (6,1593 mEq), 4 days (3,0397 mEq). Statistical analysis with Anova test showed the inhibition of Moringa leaves powder to decrease in peroxide value on used cooking oil with a value of p (sig) = 0,000 which was less than 0.05. Based on the results of the Tukey HSD test, the most effective soaking of Moringa leaves powder for 4 days was to reduce peroxide levels in used cooking oil by giving 5gr Moringa leaf powder with 100ml used cooking oil. Keywords : Levels of Peroxide Value, Moringa Leaves, Used Cooking Oil Universitas Muhammadiyah Surabaya 2019-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/3380 10.30651/jmlt.v2i2.3380 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 28-36 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/3380/2252 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/3384 2022-07-13T04:25:08Z analis:ART

Uji Daya Serap Diapers Anti Ruam (Diapers Rash) Modifikasi Sabut Kelapa (Cocosnucifera) Mardiyah, Siti The World Health Organization (WHO) reported in 2012 that the prevalence of skin irritation (diaper rash) in infants was quite high at 25% due to the use of diapers. One of the causes of diaper rash is caused by low diaper absorption. Increased absorption of diaper can be done by increasing the absorbent ability of diapers to absorb baby urine. The natural absorbent that can be modified in baby diapers is coconut fiber. Cellulose content in coconut fiber has the ability to absorb liquid high enough. This potential can be applied to absorb urine and sweat so that it can reduce moisture in baby diapers. Diapers that have high absorption can prevent the occurrence of rashes in infants. This study aims to examine differences in absorbency of diapers. Without coconut fiber and coconut husk modification diapers.             This type of research is experimental. The population and sample of the study were baby diapers with a total sample of 32 which were divided into 2 groups namely baby diapers without coconut fiber and with modification of coconut fiber. Testing for diaper absorption is carried out in a laboratory manner by measuring the volume and time of absorption of fluids given to diapers to the saturation limit (leak). The data analysis technique uses the independent t-test. Based on the results of the independent t-test showed that there were significant differences in the amount of volume and time of absorption of fluids given to bunch diapers and with coconut fiber. Thus it can be concluded that there is a significant influence on the addition of coconut fiber to the absorption of fluid in baby diapers. Keywords: Diapers, Rash, Coconut Fiber. Universitas Muhammadiyah Surabaya 2019-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/3384 10.30651/jmlt.v2i2.3384 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 47-55 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/3384/2254 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/3387 2022-07-13T04:25:08Z analis:ART

Pemeriksaan Jumlah Kapang Sebagai Indikator Kualitas Terasi Di Pasar Tambaksari Surabaya Artanti, Dita Azizah, Fitrotin The main problem that occurs in the community is the lack of awareness of the importance of hygiene during the manufacture and storage of shrimp paste. This can trigger one of the contaminations originating from microorganisms including mold. The aimed of this study was to determine the number of molds as an indicator of shrimp paste quality in the Tambaksari market in Surabaya. This research is descriptive. The population of this research is shrimp paste traders in Tambaksari market in Surabaya. The research variable is the number of molds in the shrimp paste in the packaging without a brand in the Tambaksari market in Surabaya. Data on the number of molds was tabulated and analyzed descriptively by calculating the percentage of samples that were of good quality and not good. The results of the research on the number of mold colonies on shrimp paste sold in the Tambaksari market in Surabaya showed that the number of mold colonies in shrimp paste samples was safe to consumed because the amount was <2 x 102 colonies / gr, the average percentage of all shrimp paste samples was 100% good quality. The highest growth rate of mold colonies was 19 x 101 colonies / gr and the lowest growth rate of mold colonies was 0 x 101 colony / gr. Keywords : The Number of  Mold, Shrimp paste Universitas Muhammadiyah Surabaya 2019-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/3387 10.30651/jmlt.v2i2.3387 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 56-64 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/3387/2255 Hak Cipta (c) 2019 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4012 2023-01-27T07:23:40Z analis:ART

Perbedaan Pertumbuhan Bakteri Staphylococcus aureus Dengan Pemberian Perasan Daun Anting-Anting Dan Perasan Daun Bahagia Artanti, Dita Radiawati, Eka ABSTRACT               Gram Positive Bacteria, one of which is Staphylococcus aureus is a type of bacteria that is a major cause of nosocomial infection due to surgical procedures and treatment equipment in hospitals and poisoning in several regions in Indonesia. Staphylococcus aureus is commonly found in skin, nose, mouth, eye, finger, intestinal, and liver lesions. Juice of Anting-anting leaves (Acalyhpa indica L.) and happy leaf plants (Dieffenbachia bowmanii) are known to have medical benefits. Especially in its ability to produce metabolites that function as anti-bacterial compounds. This study aims to determine the differences in the growth of Staphylococcus aureus colonies which were given the leaves of Anting-anting and the leaves of Happy leaves. The research method uses the liquid dilution method in which the Staphylococcus aureus bacteria is incubated with the leaves of the Anting-anting and the leaves of the Happy leaves with a concentration of 100% at 37 ° C overnight. Then grown on Mannitol Salt Agar (MSA) media. The results showed that there was no difference in the growth of Staphylococcus aureus bacteria colonies that had been given the treatment of Juice of Anting-anting leaf (Acalyhpa indica L.) and happy leaf plants (Dieffenbachia bowmanii) which were marked by the absence of colony growth. So that the concentration of 100% in both leaves of juice is the best concentration that can be used to control the growth of Staphylococcus aureus.  Keywords : Anting-anting Leaves, Bahagia Leaves, Staphylococcus aureus Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4012 10.30651/jmlt.v3i1.4012 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 13-22 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/4012/5961 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4013 2022-07-13T05:20:39Z analis:ART

Status Kadar Hemoglobin Dan Jenis Leukosit Pada Pasien TB Paru Di Surabaya Maulidiyanti, Ellies Tunjung Sari Abstract Tuberculosis (TB) is a global public health problem, the World Health Organization (WHO) stated when it occurred around 7-8 million TB cases with 1.3-1.6 million deaths each year (WHO, 2012). TB is a specific multi-systemic infection, which can cause diverse clinical manifestations in each organ system, so, the clinical presentation of this disease is also quite diverse. Hematologic findings in TB are not uncommon and usually occur due to non-immunological factors. Hematological disorders in the form of anemia, leukocytosis, neutrophilia, lymphopenia, lymphocytosis and increased blood slope rate (LED), thrombocytosis and thrombocytopenia. On blood tests, when TB starts to become active there is a slight leukocytosis with a slightly abnormal type of count. Anemia is reported in 16-94% of patients with pulmonary TB. One's anemia status can be assessed one of them through examination of hemoglobin. The purpose of this study was to analyze the levels of hemoglobin and types of leukocytes in pulmonary TB patients in Surabaya. This examination uses an automatic analyzer with the working principle of SLS (sodium lauryl sulfate). Samples of the blood of pulmonary TB patients in several hospitals in Surabaya .. From 30 samples obtained an average hemoglobin level of 11.5 gr / dl, as many as 10 (33.4%) samples whose levels were within normal limits and 20 (66.6%) sample levels were below normal values. Examination of leukocyte count showed that there were 8 samples (26.6%) of normal neutrophil cells, 22 samples (73.4%) abnormal, 5 lymphocytes of normal cells (16.6%), 25 samples (83% abnormal), abnormal 25 samples (83%) , 4%), normal monocyte cells were 17 samples (56.6%) and abnormal were 13 samples (43.4%).  Keywords: haemoglobine, leukosit type, TB patients Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4013 10.30651/jmlt.v3i1.4013 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 53-60 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/4013/2855 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4014 2022-07-13T05:20:39Z analis:ART

Status Kadar Kolesterol Terhadap Kebiasaan Orang Yang Mengonsumsi Teh Purwaningsih, Nur Vita ABSTRACT Lifestyle must be a trend nowadays, many high cholesterol foods needed in daily life can increase blood cholesterol and increase the risk of cardiovascular disease. One way to reduce cholesterol in the body is by consuming tea. Tea has a very beneficial content for the body. One of the contents of the tea is catechins. Catechins are secondary metabolites that are naturally produced by plants belonging to the flavonoid class, which are made to help absorb calcium in the intestine. This type of research is observational analytic samples collected are students who have the habit of consuming tea by doing two examinations that is before and observing observations for 3 months. Based on the results of the study obtained an average cholesterol level before the observation of the results obtained 152.9 mg / dl while for the temporary examination of the results obtained 141.3 mg / dl. The results of data analysis using the Paired T Test with sig 0.00 which means less than 0.05. So, it can be concluded that there is an influence of tea consumption habits on cholesterol levels. Keywords: cholesterol, habits in consuming tea Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4014 10.30651/jmlt.v3i1.4014 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 23-28 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/4014/2852 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4630 2022-07-13T05:20:39Z analis:ART

Pengaruh Ekstrak Mengkudu (Morinda citrifolia) Terhadap Penurunan Tekanan Darah Pada Tikus Putih Wistar Jantan Dengan Hipertensi W, Pipit Festi Hypertension is one of the most important causes of early death because it is closely related to the risk of cardiovascular disease. The prevalence of hypertension continues to increase with lifestyle changes. Non-pharmacological management is an alternative in the treatment of hypertension, one of which is complementary therapy by consuming herbal ingredients. The purpose of this study was to determine the effect of noni extract on reducing blood pressure in male  Wistar experimental-rats.The research design used was pre experimental design. One group pre-test post test design with purposive sampling technique. Number of samples were 16 male Wistar experimental-rats . The instrument in this study was the observation sheet and blood pressure measurement for taicap blod pressure. Statistical test analysis using Wilcoxon test.The results showed blood pressure before being given noni extract, cystolic pressure 150.93 mmHg, diastole pressure 112.87 mmHg. After administration of noni extract average blood pressure systole: 110,625 mmHg, diastole blood pressure average 85,125 mmHg Wilcoxon statistical test results showed that p = 0,000 <a = 0.05 showed a decrease in blood pressure before and after giving of noni extractThe conclusion from the study showed that there was an effect of noni extract on reducing blood pressure in hypertensive male Wistar experimental-mouse. Development of natural ingredients in the management of non-pharmacological hypertension needs to be done at the dose and effectiveness.                      Keywords: Hypertension, Noni extract, white rat.  Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4630 10.30651/jmlt.v3i1.4630 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 1-12 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/4630/2850 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4632 2022-07-13T05:20:39Z analis:ART

Gambaran Parasit Cacing Nematoda Usus Dan Cestoda Pada Feses Sapi (Boss sp.) Di Peternakan Sumber Jaya Ternak, Kecamatan Tikung, Kabupaten Lamongan, Jawa Timur Arimurti, Anindita Riesti Retno Cows are important animals for Indonesian farmers who have high economic value. Cow breeding business has many benefits for human life, especially meat, milk, bones, skin, offal and feces (cow dung). Cow feces can be used as an organic fertilizer manufacture by cattle farmers. But it needs to be considered in making organic fertilizer derived from cows because it contains many diseases and parasites. If the cow's feces are not treated properly, it will cause environmental pollution to the local residents. Cow infected with parasites can experience a decrease in body weight, decreased endurance, impaired growth and death. To find out intestinal helminth parasitic infections, one of them is by identifying worms in cow feces. The majority of the population of Tikung Subdistrict, Lamongan Regency, earn their living as farmers and ranchers. The purpose of this study was to identify intestinal Nematode worms in cow feces in Tikung Subdistrict, Lamongan Regency. The method used in this study was Nacl Saturated to determine the morphology and forms of parasites. The results showed that cattle feces samples taken from the Tikung Subdistrict of Lamongan District contained the Nematoda parasite, which was found the presence of Hookworm eggs in 3 samples with a percentage of 6% and found 1 positive sample (+) containing Taenia saginata eggs, with a percentage of 2% of the total 50 samples in Sumber Jaya Livestock Farm in cattle on Tikung Subdistrict, Lamongan Regency is still relatively low. Keywords: Cow Feces (Bos sp.), Intestinal Nematodes, Cestodes Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4632 10.30651/jmlt.v3i1.4632 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 39-52 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/4632/2854 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4765 2022-07-13T05:20:39Z analis:ART

Hubungan Paparan Pestisida Terhadap Kadar Trigliserida Pada Petani Di Daerah Mojokerto Santoso, Andreas Putro Ragil Wulandari, Devyana Dyah Wulansari, Devyani Dyah Alzheimer’s chronic generational neuro disease is indicated by the loss of neurons and synapses, factors that cause Alzheimer’s include cholesterol such as triglycerides and poisoning due to pesticides due to pesticides due to spraying activity. This study aimed to determine the extent of pesticide exposure to triglyceride levels in farmers in the Mojokerto area. This research is an observational type of research with an experimental approach. This research was conducted in Sumbersono Village, Dlanggu Subdistrict, Mojokerto Regency with 25 farmers who carried out spraying activities. This study uses a correlation test that is the Spearman's test with a confidence level of p < 0.05. Based on results of the study showed that farmers who carried out pesticide spraying activities with a working period of up to more than 15 years with a long time under the field around 3-6 hours but did not use personal protective equipment properly. The results of the average triglyceride levels of 158.2 mg / dL Spearman's test showed triglyceride hash to work mas amounted to 0.899, to the length of work of 0.442 and to the use of PPE 0.811 it showed p > 0.05 which means there was no relationship between farmers who carried out spraying activities with triglyceride levels.Keyword: Farmer, Spraying activity, Triglycerides Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4765 10.30651/jmlt.v3i1.4765 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 61-68 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/4765/2856 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4770 2022-07-13T05:20:39Z analis:ART

Validasi Dan Verifikasi Hasil Uji Sun Protection Factor (Spf) Pada Sediaan Sunblock Dan Sunscreen Bermerk Dengan Metode Spektrofotometri yuan, rahayu artini Tests for the determination of SPF value were calculated from the seven sunblock and brandes sunscreen preparation which were analyzed using 96% etanol solvent and kloroform, ranging from 17.21-26.88. The results of the VA-14 sample calculation have the lowest SPF value (SPF = 17.21) and the ES-30 sample has the largest SPF value calculation results from the seven samples (SPF ES-30 = 26.88). The seven samples are samples that have well-known brands in Indonesia. Of the seven cosmetics samples, six samples had a% recovery in accordance with the standard between 80-120%, but one product, namely SA-35, had a recovery rate of 73.23%. Organoleptic test results showed that the overall type of cream was m / a, fragrance, color from milky white, light brown, and light green. The pH value between 6.8-7.5 according to the quality standard of sunscreen preparations in SNI 16-4399-1966, the pH value for sunscreen preparations is 4.5-8.0. Thus, the results of the seventh pH of the sample are still within good standard limits for cream preparations containing sunscreen. Universitas Muhammadiyah Surabaya 2020-06-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4770 10.30651/jmlt.v3i1.4770 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 29-38 2614-2805 2597-3681 10.30651/jmlt.v3i1 ind https://journal.um-surabaya.ac.id/analis/article/view/4770/2853 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/4782 2022-12-26T06:08:52Z analis:ART

PENETAPAN KADAR VITAMIN B1 PADA GENJER (LIMNOCHARIS FLAVA) DENGAN PENGUKUSAN MENGGUNAKAN SPEKTROFOTOMETER UV-VIS Rahmawati, Previta Zeisar Sa'diyah, Dewi Cholimatus ABSTRACTGenjer (Limnocharis flava) is a daily consumable plant that often used as vegetable by wide society. It known have completed nutritional content such as protein, carbohydrate, mineral and vitamin. Genjer also known as good source of B1 vitamin. The purpose of this study was to identify B1 vitamin level of fresh and steamed genjer plant. Genjer plant were steamed for 2 minutes and 4 minutes. We also want to examine the best steamed time for the plant towards B1 vitamin level. Determination of genjer B1 vitamin level wa used UV-Vis Spectrofotometry at 511 nm, and thiamine was used as the standart solution. The study shown that B1 vitamin level were o,121 mg/100 g, 0,035 mg/100 g and 0,027 mg/100 g in fresh, 2 minutes steamed and 4 minutes steamed genjer plants respectively. We were done a quantitatively analysis of collected data to describe B1 vitamin level in treated genjer plants. The result showed that the longer of steamed period will reduce more of B1 vitamin level at genjer plants. We suggested that B1 vitamin more soluble in water and processing technique related with water have an effect towards B1 vitamin level, and 2 minutes steaming reduce less of B1 Vitamin compared to 4 minutes steaming. Keywords: Genjer (limnocharis flava), Analysis of Vitamin B1 Levels, UV-Vis Spectrophotometer Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/4782 10.30651/jmlt.v3i2.4782 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 1-10 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/4782/3410 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/5374 2022-12-26T06:08:52Z analis:ART

Efektivitas Antibakteri Sediaan Sabun Bunga Gemitir (Tagetes erecta L.) Terhadap Staphylococcus aureus dan Escherichia coli Cahyaningrum, Putu Lakustini Yuliari, Sang Ayu Made Mediastari, AA Putu Agung Penelitian ini bertujuan untuk mengetahui efektivitas antibakteri formulasi sediaan sabun padat ekstrak bunga gemitir (Tagetes erecta L.) dan mengetahui baku mutu SNI dari sabun antibakteri yang dihasilkan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 formula dan 3 kali ulangan. Formula dalam penelitian ini adalah penambahan ekstrak bunga gemitir (Tagetes erecta L.) sebesar 0; 1; 1,5; 2 %. Uji aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli dilakukan dengan metode difusi sumur. Sedangkan uji kualitas sabun ditentukan dengan uji organoleptis, kadar air, pH, asam lemak bebas/alkali bebas dan lemak tak tersabunkan. Hasil penelitian menunjukkan bahwa ekstrak bunga gemitir (Tagetes erecta L.) dapat diformulasikan dalam sediaan sabun padat dan menunjukkan aktivitas antibakteri dengan semua formulasi kategori sedang terhadap bakteri S. aureus dan E. coli dengan diameter zona hambat terbesar adalah formula F3 (penambahan ekstrak bunga gemitir 2%), yaitu 18,567 mm terhadap bakteri gram positif S. aureus dan 19,80 mm terhadap bakteri gram negatif E.coli. Hasil Uji kualitas sabun padat ekstrak bunga gemitir sesuai dengan standar SNI. Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/5374 10.30651/jmlt.v3i2.5374 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 11-24 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/5374/3411 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/5841 2022-12-26T06:08:52Z analis:ART

Analisis Kadar SGPT (Serum Glutamic Pyruvic Transaminase) Dan Kholinesterase Pada Petani Sayur Di Desa Riang Gede, Kecamatan Penebel, Kabupaten Tabanan Sukmayanti, Ni Luh Putu Ari Artini, Ni Putu Rahayu Yanti, Ni Putu Wida Excessive users in the long term can cause damage to liver function qhich is indicated by the presence of SGPT levels that exceed the reference value and cholinesterase levels that are less than the reference value. The purpose of this study was to determine the levels of SGPT and kholinesterase in vegetable famers in Riang Gede Village, Penebel District, Tabanan Regency. This research is a descriptive study using a statistical test that displays the lowest, highest value, average test results and deviation standards to correct errors in the analysis process. Blood sampling is done on 30 vegetable farmers by using a sampling technique in the form of random sampling SGPT and kholinesterase levels measured using a photometer, the results of the study showed an average SGPT level of 17,0 ± 0,02 U/L, the highest level of 48,0 ± 0,05 U/L and the lowest of 9,0 ± 0,05 U/L. The average level of cholinesterase is 5,9 +0,02 kU/L, the highest level is 8,0 ± 0,05 kU/L and the lowest level is 3,5±0,05 kU/L. Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/5841 10.30651/jmlt.v3i2.5841 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 25-31 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/5841/3412 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/5843 2022-12-26T06:08:52Z analis:ART

ANALISI KADAR ALP (ALKALINE PHOSPATASE) DAN KHOLINESTERASE AKIBAT LAMA BEKERJA PADA PETUGAS FOGGING DI KOTA DENPASAR Hendriani, Ni Kadek Mona Fujiastuti Artini, Ni Putu Rahayu Aryasa, I Wayan Tanjung  Fogging is an effective way to prevent dengue fever in Indonesia. The side effects of fogging can have an impact on its officers by being exposed and inhaled by continuing to treat fumes that contain pesticides. So, that the liver function examination is carried out to determine the increase in ALP  (Alkaline Phospatase ) levels and decrease in cholinesterase levels due to a long time working on fogging officers in Denpasar. This aims to determine whether there is an increase in ALP levels and a decrease in the level of cholinesterase in fogging officers' blood samples. This type of research carried out statistical tests by displaying the lowest, highest value and average test results and deviations standards. From the examination results obtained, it was found that ALP levels in March 2020 with an average of 63.1 ± 0.23 IU / L, while in April 2020 with an average of 98.0 ± 0.23 IU / L, at cholinesterase levels in March 2020 with an average of 5.2 ± 0.01 kU / L, while in April 2020 the average was 6.5 ± 0.01 kU / L. It can be concluded that there was an increase in ALP with an average percentage increase of 0.88% and a decrease in cholinesterase levels in April higher than March, with an average percentage decrease of 0.29%.   Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/5843 10.30651/jmlt.v3i2.5843 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 32-39 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/5843/3413 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/5853 2022-12-26T06:08:52Z analis:ART

EFEK PAPARAN KRONIS PESTISIDA TERHADAP KADAR ASPARTAT AMINOTRANSFERASE (AST) DAN ALANIN AMINOTRANSFERASE (ALT) PADA SUBYEK PETANI Wulandari, Devyana Dyah Ragil Santoso, Andreas Putro Wulansari, Devyani Diah Farmers in Indonesia are very dependent on pesticides to protect their crops from pests. Farmers are one of the subjects who are susceptible to pesticides and can cause disease due to pesticide accumulation. One of the disease that can arise due to exposure to pesticides is liver damage. This study wanted to determine the effect of pesticide exposure on liver damage seen from AST and ALT enzyme levels using farmer respondents in the Mojokerto area of 25 respondents. From the results of the study it was found that AST levels were influenced by age (P = 0.017), length of work (P = 0.035), and PPE usage (P = 0.044), while age, work period, length of work and usage of PPE did not significantly influence to ALT levels. It can be concluded that age, length of work and use of PPE affect AST levels. Therefore, to prevent the occurrence of pesticide poisoning, farmers must obey the rules on the use of pesticides such as using PPE regularly and always consuming healthy food. Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/5853 10.30651/jmlt.v3i2.5853 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 39-48 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/5853/3447 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/5860 2022-12-26T06:08:52Z analis:ART

GAMBARAN KADAR UREUM DAN KREATININ PENDERITA PENYAKIT GINJAL KRONIK (PGK) YANG TERINFEKSI MALARIA DI RUMAH SAKIT UMUM DAERAH JAYAPURA kayoi, sabakthani Artini, Ni Putu Rahayu Aryasa, I Wayan Tanjung The aim of the study was to see the comparison of urea and creatinine levels in chronic kidney disease patients between those infected with malaria and those not infected with malaria at the Jayapura Regional General Hospital. This type of research is retrospective analytic. The samples of this study were chronic kidney patients who were infected with malaria and conducted an examination at the Jayapura Regional General Hospital that met the inclusion criteria, in order to obtain 30 positive malaria samples. The average urea level of female CKD patients infected with malaria was 116.7 mg/dL, the lowest was 63,8 mg/dL and the highest was 136,2 mg/dL. The average urea level of male CKD patients infected with malaria was 95,9 mg/dL, the lowest was 33.4 mg/dL and the highest was 231,0 mg/dL. The result of the mean creatinine level of female CKD patients infected with malaria was 17,5 mg/dL, the lowest was 9,92 mg/dL and the highest was 31,38 mg/dL. While the mean creatinine level of male CKD patients infected with malaria was 12,0 mg/dL, the lowest was 3,49 mg/dL and the highest was 26,27 mg/dL. Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/5860 10.30651/jmlt.v3i2.5860 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 49-56 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/5860/3415 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/5861 2022-12-26T06:08:52Z analis:ART

GAMBARAN NILAI SATURASI OKSIGEN (SO2) DENGAN TEKANAN OKSIGEN (PO2) PADA PASIEN PENYAKIT JANTUNG KORONER (PJK) DI RUMAH SAKIT UMUM DAERAH JAYAPURA Kadam, Paulina Artini, Ni Putu Rahayu Aryasa, I Wayan Tanjung Coronary heart disease (CHD) patients are equipped with blood tests to reinforce the diagnosis of the patient's disease. One of the parameters that is usually checked is blood gas analysis (AGD). One of the parameters of blood gas analysis (BGA) is oxygen saturation (SO2), oxygen pressure (PO2), and carbon dioxide pressure (PCO2). The purpose of this study was to determine the relationship between SO2, and PO2,at the Jayapura Regional General Hospital. This type of research is retrospective analytic. The sample of this study were coronary heart patients who underwent BGA xamination at the Jayapura General Hospital who met the inclusion criteria, so that 30 samples were obtained. The type of data is secondary data. With the mean value of SO2 is 96,1% and PO2 is 133,2 mmHg. The results of the relationship between SO2 and PO2 in coronary heart disease patients at Jayapura Regional Hospital are directly proportional, namely the decrease in SO2, PO2 will also decrease in CHD patients, with four people experiencing moderate hypoxemia, five people with normal PO2, and 21 people with PO2 is high because you have got a ventilator device. Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/5861 10.30651/jmlt.v3i2.5861 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 57-65 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/5861/3416 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6047 2022-12-26T06:08:52Z analis:ART

THE EFFECT OF TOMAN (Chana Micropetes) EXTRACT ON THE IMPROVEMENT OF ALBUMIN LEVELS IN MENCIT/MICE (Mus musculus) W, Pipit Festi Hypoalbuminemia is a condition in which albumin levels are less than 3.8 g / dl. Management of hypoalbumin can be done on a  high high in protein such as toman fish extract (Chana Micropetes). Toman fish extract contains globular protein which can be used as an alternative diet in increasing albumin levels. The purpose of this study was to determine the effect of toman fish extract on increasing albumin levels in mencit (Mus musculus).               This research is an experimental research design with Pretest-Posttest Control Group Design. Samples using Mus musculus with the criteria of male sex, body weight 25-40 grm and 2 months old. The number of samples consisted of 32 animals consisting of 2 groups, 16 control groups and 16 treatment groups. Intervention by giving toman fish extract for 14 days. Research instruments, albumin levels and observation sheets. Data were tested statistically with Independent Sample t Test.               The results, this study showed the average value of albumin levels was 5.60 gr / dl in the treatment group so that an increase in albumin levels. There was a significant effect (p = 0,000 <α = 0.05) between the administration of toman fish extract to the increase in albumin levels in mencit (Mus Muculus).               Conclusion, giving toman fish extract can increase albumin levels in mencit. So that toman fish extract can be used as a high-protein alternative diet for hypoalbumin sufferers.Keywords: hypoalbumin, toman fish, (Chana Micropetes). Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6047 10.30651/jmlt.v3i2.6047 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 66-77 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/6047/3417 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6065 2022-12-26T06:08:52Z analis:ART

ANALISIS GEN TCF7L2 (Trancription Factor 7 Like 2) PADA KELUARGA PENDERITA DIABETES MELLITUS TIPE 2 KECAMATAN TANGGULANGIN, KABUPATEN SIDOARJO Mushlih, Miftahul Iknan, Siti Asriani Amin, Hindah Sabrina Cholifah, Siti Segara, Bayu Diabetes mellitus is a metabolic disorder characterized by not produced insulin or disruption of insulin action. Diabetes mellitus was reported correlated with TCF7L2 gene mutation. This study aimed to analyze the TCF7L2 gene in families with type 2 diabetes. 5 samples were used in this study, the methods flow through DNA Isolation, PCR, and sequencing. Samples were amplified by the PCR technique using primers that were designed to detect the TCF7L2 gene, (F: 5'-GGCTTGATTGTTGATTATGGGC3' and R:5 'TCTGGCACTCAGAGAGT 3'). The amplification and sequencing resulted in 368 bp (located at 103631-103972 bp). We found a transition mutation at C103950T. The polymorphisms found may characteristic in this population because they are not found in other sequences. Keywords : Diabetes mellitus, TCF7L2 gene, mutation Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6065 10.30651/jmlt.v3i2.6065 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 78-85 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/6065/3426 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6140 2022-12-26T06:08:52Z analis:ART

HUBUNGAN EDEMA DENGAN PROTEIN URINE PADA IBU HAMIL DI RSU PRIMA HUSADA SIDOARJO Santoso, Andreas Putro Ragil Masruroh, Nur Ibu hamil memiliki resiko yang tinggi dengan adanya penyakit komplikasi berupa sindroma HELLP (hemolysis, elevated, liver enzyme, low platelet) edema, gangguan ginjal, perdarahan, infeksi, tekanan darah tinggi saat kehamilan, komplikasi persalinan, aborsi hingga kematian. Preeklamsi terjadi akibat peningkatan hipertensi dimana sebelumnya tidak pernah mengalami hipertensi sebelum kehamilan. Preeklamsi menimbulkan proteinuria dan edema. Penelitian ditujukan untuk mengetahui hubungan kadar protein urine dengan edema pada Ibu Hamil. Desan penelitian menggunakan pendekatan cross sectional. Populasi penelitain merupakan pasien dari RSU Prima Husada, dengan jumlah sampel minilam sebanyak 30 sampel. Hasil penelitian yang dilakukan uji berdasarkan uji spearman menunjukkan bahwa signifikansi p> 0,05 yaitu 0,456 hal tersebut menunjukkan bahwa tidak ada hubungan antara edema dengan protein urine pada Ibu hamil. Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6140 10.30651/jmlt.v3i2.6140 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 86-93 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/6140/3419 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6167 2022-12-26T06:08:52Z analis:ART

PREVALENSI HELMINTIASIS PADA SISWA KELAS 1 – 6 SEKOLAH DASAR MANYAR SABRANGAN SURABAYA TAHUN 2020 Lukiyono, Yauwan Tobing SUMARSONO, THOMAS - murhadjito, imma rachmawati Worming diseases are still a public health problem in Indonesia. This infection is still often found in cases in Southheast which is in Indonesia. Factors the geographical location on Indonesia,Which has a tropical climate and sufficient humidity, is very supportive for the development of parasites. Primary school age is a group that is often exposed to helminthiasis because is is often assosiated with soil contaminated with worm egg.This study aims to determine the prevalence of worm egg parasitic infections in the feses of student in grade 1 to grade 6 of the manyar sabrangan 231 surabaya elementary school in 2020.The research sample was faeses of grade student 1 – 6 Manyar Elementary school 231 Surabaya.Identification of worm eggs is carried out in E – Labz Telcomedika Surabaya Elementary. Data taken in the form of parasitic worm eggs based on morphological characteristics.From the result of study found in 240 elementary scholl children examined, as many as 29 children (12,1 %) positive for helminthiasis, with parasite rate of 18 children (7,5%) Ascaris lumbricoides, 7 children (2,92%) Trichiuris trichiura. The conclusion of this study, helminthiasis of student  in grade 1 to grade  6 of the Manyar Sabrangan Surabaya Elementary School were found ,namely Ascaris lumbricoides,Trichiuris trichiura Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6167 10.30651/jmlt.v3i2.6167 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 94-99 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/6167/3420 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6467 2022-07-13T05:23:37Z analis:ART

Evaluasi Analitik Hematology Analyzer Diatron Abacus 3 Pada Parameter Hematologi Rutin Di Laboratorium Hematologi Poltekkes Kemenkes Kalimantan Timur Kesuma, Suryanata Syumarliyanty, Mahdiah Hartono, Agus Rudi Entering the era of globalization, manual tools in clinical laboratories have been replaced by full automatic devices. One of them is the Diatron Abacus Hematology analyzer 3. A relatively new hematology analyzer is required for analytical evaluation. Analytical evaluation is an evaluation of Diatron Abacus 3 on Sysmex KX 21 as a standard in RSUD I.A Moeis Samarinda, and it is very important to do this to assess the performance of the tool. Analytical evaluation is done by determining the value of accuracy, precision, and total error and linearity of measurement results from routine hematological examination parameters, which are erythrocytes, leukocytes, platelets, hemoglobin, and hematocrit. To determine the results of the hematology analyzer evaluation of Abacus 3 Diatron Analyzers on Routine Hematology Parameters in the Hematology Laboratory of the Health Ministry of Health, East Kalimantan. This type of research is observational descriptive, using a total sampling technique, and a sample of 40 complete K3EDTA blood specimens. Data processing using Microsoft Excel and SPSS 20 applications, analyzed using descriptive statistics. The observations were still included in the criteria for acceptance, accuracy or inaccuracy (d%) in erythrocytes 1.8%, leukocytes 8.0%, platelets -5.3%, hemoglobin 2.3% and hematocrit -1.7%; Precision or impression (CV%) in erythrocytes 4.2%, leukocytes 11.1%, platelets 6%, hemoglobin 3.9% and hematocrit 4.5%; Total errors in erythrocytes were 8.7%, leukocytes 17.9%, platelets 23.6%, hemoglobin 8.8% and hematocrit 9.1; Linearity of the measurement results against the routine hematological cell count values performed using Abacus 3 and Sysmex KX 21 has a positive relationship. Acceptance values are still included in the LOA on all parameters examined and still meet the criteria; the accuracy/bias value is smaller than the true value of the parameter being examined, except for leukocytes, which is greater; the precision on the five parameters is greater than the CV% Abacus 3 insert kit; The total error obtained by the TE value is greater than the TEA in the parameters examined, except for smaller platelets; Linearity of the measurement results against the calculated hematology cell routine values performed using Abacus 3 and Sysmex KX 21 have a positive relationship, meaning an increase in measurement values using Abacus 3 is followed by an increase in measurement values using Sysmex KX 21. Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6467 10.30651/jmlt.v4i1.6467 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 1 - 20 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/6467/3935 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6628 2022-10-31T02:39:03Z analis:ART

Description Of Ciprofloxacin Sensitivity On Bacteria Causing UTI In T2DM Patients Nazari, Muhammad Ibnu Diabetes mellitus is a chronic metabolic disease characterized by a state of hyperglycemia due to a lack of insulin production by the pancreas or the body's inability to use the insulin produced or both. Uncontrolled hyperglycemia can suppress the immune system and making the body vulnerable to infection, especially urinary tract infections (UTIs). The main choice in UTI treatment is antibiotic therapy, one of the most frequently used is ciprofloxacin from the fluoroquinolone group.The research design used was descriptive method with prevalence studies. The research was done using antibiotic sensitivity tests with agar diffusion methods. The number of samples were taken using total sampling method.The results of ciprofloxacin sensitivity test were obtained as many as 22 isolates. Escherichia coli bacteria are sensitive to ciprofloxacin at 66.67%, intermediate and resistant 16.67% respectively. Pseudomonas aeruginosa are 60% sensitive and 40% intermediate. Enterobacter aerogenes are 66,67% sensitive and 33,33% intermediate. Shigella spp. are 100% sensitive, and Klebsiella spp. 100% resistant.The conclusion from the study showed that ciprofloxacin are an effective empirical therapy for UTI. Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6628 10.30651/jmlt.v5i1.6628 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 8-14 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/6628/5087 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6672 2022-12-26T06:08:52Z analis:ART

KARAKTERISTIK WANITA USIA SUBUR DENGAN TINDAKAN PENCEGAHAN KANKER SERVIKS Supatmi, Supatmi AbstractBackground:  The awareness of Indonesian women to carry out early detection of cervical cancer on a regular basis have been still low. Cervical cancer can be prevented and cured by taking early detection measures because it has a long preinfassive phase. Cervical cancer prevention measures are influenced by several factors including age, education level, occupation and parity, Aims:   analyze the characteristics of women of childbearing age with cervical cancer prevention measures (HPV, IVA and Pap smear immunization) Methods: Quantitative analytic research design with cross sectional approach. The population was 193 women of fertile age in RW 9 Kelurahan Kapasan Surabaya with n Cluster Samples, the sample was 130 WUS. The research instrument used a characteristic questionnaire and cervical cancer prevention measures with a Dichotomy Question. The research variables were WUS cataristic and cervical cancer prevention behavior. Analysis with Chi-Square with a significance level of 0.05Results:  There was a relationship between age and HPV immunization (p = 0.066) and pap smear (p = 0.058), IVA action had not relationship (p = 0.770), education was related to HPV immunization (p = 0.346). IVA (0.005) was not related to pap smears = 0.603) Occupation was related to HPV immunization (p = 0.280), IVA (p = 0.000) and pap smears (p = 0.216), parity was related to HPV immunization (p = 0.003) ) and Pap smear (p = 0.117) meanwhile parity had not relationship with IVA (p = 0.839). Studying the characteristics of WUS is a first step to increase preventive efforts for cervical cancer both primary and secondary prevention   Keyword:  Characteristics of WUS, Prevention, Cervical Cancer Universitas Muhammadiyah Surabaya 2020-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6672 10.30651/jmlt.v3i2.6672 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 3 No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist; 100-107 2614-2805 2597-3681 10.30651/jmlt.v3i2 ind https://journal.um-surabaya.ac.id/analis/article/view/6672/3421 Hak Cipta (c) 2020 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/6949 2022-07-13T05:23:37Z analis:ART

UJI KANDUNGAN HIDROQUINON PADA SEDIAAN KRIM RACIKAN DOKTER DAN KRIM PENCERAH WAJAH DENGAN MENGGUNAKAN SPEKTROFOTOMETER UV yuan, rahayu artini Hydroquinon is a chemical used as a skin lightener. However, its effectiveness began to be questioned after the emergence of a study showing that hydroquinone can trigger cancer. This study aims to analyze the hydroquinone content of several doctor-made creams and branded facial lightening creams circulating in Denpasar. This research is an experimental research. The sample used consisted of five samples of doctor-made creams coded D1-D5, while branded lightening creams were coded P1-P10. Based on the results of the analysis, the wavelength of 293 nm is the wavelength that provides maximum absorption with the equation y = 0.023x + 0.005 with a value of R2 = 0.996. 0.023. The results of the analysis showed that all samples of the doctor's cream contained hydroquinone with an average of 11.9322 ppm or 1.1932% and did not exceed the BPOM regulations, namely 5% for cream preparations prescribed by doctors. And all samples of branded facial lightening creams in Denpasar City contain hydroquinone with an average hydroquinone level of 3,987 ppm or 0.3987% and do not exceed the BPOM regulations, which is 2% for whitening cream preparations that are sold freely without a doctor's prescription.  Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/6949 10.30651/jmlt.v4i1.6949 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 30 - 39 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/6949/3937 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7242 2022-07-13T05:23:37Z analis:ART

Korelasi Profil Darah Dengan CRP Serum pada Pasien Diabetes Mellitus dengan Ulkus Diabetikum Aliviameita, Andika Puspitasari, Puspitasari Purwanti, Yanik Diabetes mellitus is a metabolic disorder that experiences hyperglycemia and causes various complications, including diabetic foot ulcers (DFU). Hyperglycemia can cause chronic inflammation and decreased function of immune cells. This study aims to determine the correlation between blood profiles and serum C-Reactive Protein (CRP) in diabetes mellitus patients with diabetic ulcers. A total of 30 blood samples of diabetes mellitus patients with blood tests and serum CRP. Blood profile examination used an automatic method with a hematology analyzer, erythrocyte sedimentation rate (ESR) using the Westergreen method, and serum CRP using a semi-quantitative method. In the  correlation regression multiple test knowed that the parameters associated with CRP serum are hemoglobin (r = 23.553; p = 0.015), hematocrit (r = -8.678; p = 0.016), leukocyte count (r = 2.098; p = 0.004), and platelet count (r = -0.112; p = 0.002) because it has significance (p < 0.05). Meanwhile, the parameters of erythrocyte count (r = -8.717; p = 0.359), and ESR (r = -0.142; p = 0.109) had no effect on serum CRP levels because they had significance (p> 0.05). The conclusion of this study shows there is a strong correlation (r = 0.777) between blood profile and serum CRP in diabetes mellitus patients with diabetic foot ulcers. Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7242 10.30651/jmlt.v4i1.7242 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 40 - 48 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7242/3938 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7269 2022-07-13T05:23:37Z analis:ART

PENGARUH EKSTRAK BAWANG HITAM TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA MENCIT W, Pipit Festi  Administration of oral hypoglycemic drugs and insulin is one of the most common pharmacological treatments given to people with diabetes mellitus. This treatment is carried out in the long term so that it causes side effects and is carried out continuously so that it will affect the financial condition of the diabetes mellitus patient. The purpose of this study was to determine the effect of black garlic extract on decreasing blood glucose levels in mice (mus musculus). This study uses the design of True Experimental design (pretest-posttest with control group design). The population is mice in Pusvetma Surabaya. Sampling method uses Non Probability Sampling Purposive Sampling type. Samples taken were 30 mice consisting of 15 intervention groups and 15 control groups. Data was collected using observation sheets, then analyzed using Independent sample t test and Dependent sample t test with a significance level of 5 0.05. The results showed: Using the test Independent sample T test obtained a significance value ρ = 0,000  It can be concluded that there is an effect of black onion extract to reduce blood glucose levels in mice. In the paired t-test test in the treatment group showed ρ = 0,000  that there were differences in blood glucose levels before being given extracts of garlic and after being given intervention. It is expected that black garlic can be used as an alternative medicine to reduce blood glucose levels.Keyword : Ekstrak of black garlic, Blood sugar levels, mus musculus Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7269 10.30651/jmlt.v4i1.7269 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 49 - 65 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7269/3939 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7290 2022-07-13T05:23:37Z analis:ART

KORELASI ANTARA PROFIL HEMATOLOGI DENGAN PROCALCITONIN PADA PASIEN TERKONFIRMASI COVID-19 Puspitasari, puspitasari Aliviameita, Andika Rinata, Evi Nadila, Dira Covid-19 is an infectious disease caused by SARS-CoV 2 and has spread very quickly. Several laboratory examinations were carried out on Covid-19 patients including molecular examinations, hematological profiles, and serology. This study aims to determine the relationship between leucocyte, d-dimer, hemoglobin and thrombocyte against procalcitonin in patients with Covid-19. This was an observational analytic study with cross sectional design. A sample of 50 was taken selectively at Aisyiyah Siti Fatimah Tulangan Hospital in November 2020 - January 2021. Multiple linear regression test results showed relationship between leucocyte with procalcitonin (b = -0.64; p = 0.426), d-dimer with procalcitonin (b = 2.702; p = 0.010), hemoglobin with procalcitonin (b = 0.656; p = 0.255), and thrombocyte with procalcitonin (b = 0.037; p = 0.002) . Based on the results, it is found that there is relationship between leucocyte, d-dimer, hemoglobin, and thrombocyte againts procalcitonin in Covid-19 patients. Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7290 10.30651/jmlt.v4i1.7290 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 66 - 73 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7290/3940 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7362 2022-07-13T05:23:37Z analis:ART

UJI ANTIFUNGI KETEPENG CINA (Cassia alata L.) TERHADAP Trichophyton rubrum DAN Candida albicans Lathifah, Qurrotu A'yunin Puspitasari, Eka Turista, Dora Dayu Rahma Ketepeng Cina merupakan salah satu tumbuhan liar di Indonesia yang berpotensi sebagai antijamur. Pengujian antijamur dilakukan dengan cara difusi cakram menggunakan ekstrak dengan konsentrasi 20%, 40%, 60%, dan 80%. Hasil pengujian menunjukkan zona hambat pada semua konsentrasi ekstrak dan zona terluas dihasilkan pada konsentrasi 90%. Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7362 10.30651/jmlt.v4i1.7362 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 74 - 80 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7362/3941 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7447 2022-07-13T05:23:37Z analis:ART

Vacutainer serum separator sebagai alternatif tabung penampung darah pada pemeriksaan kadar ureum Setiawan, Budi Nugraheni, Ulfah Restu Rahayu, Muji ABSTRACT Serum using is preferred for urea level because it does not use anticoagulants which can interfere with activity and  reaction to the results. The tubes that are widely used to collect blood into serum are  vacutainer serum separator and  vacutainer plain.This researche aims to determine the degree of agreement s between vacutainer serum separator and vacutainer plain usage on serum urea level result.This research was cross sectional design and hold on October 2020 with subject were taken from  thirty blood samples of health analyst students which taken randomly and had no history of disease or kidney function disorder. Each student was taken 6 ml of blood drawn using a venoject with each vacutainer containing 3 ml, so we had 60 data. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement 0.745. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement was 0.745. The calculation of the average working time between the vacutainer serum separator and the vacutainer plain was 4 minutes 38 seconds and 35 minutes 58 seconds. The analysis concluded that the vacutainer serum separator and the vacutainer plain could be used as an alternative of blood collecting tubes for urea level testing which proved to be no significant difference in the results from this research. Keywords : Urea level,  Vacutainer Serum Separator,  Vacutainer Plain Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7447 10.30651/jmlt.v4i1.7447 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 81 - 87 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7447/3942 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7509 2022-07-13T05:23:37Z analis:ART

Uji Antioksidan Belimbing Wuluh dan Ampas Nanas Terhadap Kualitas Minyak Goreng Habis Pakai Mardiyah, Siti Anti-Oxidant Tests of starfruit and pineapple pulp on the quality of Used Cooking Oils   ABSTRACTUsed Cooking Oil is oil produced from frying residue and is carcinogenic because it causes oxidation, hydrolysis, and polymerization to produce toxic peroxides and free fatty acids that are difficult for the body to digest. Measurement of oil quality can be measured through 3 parameters, namely, the peroxide number, the acid number and the oil color. Efforts to control the quality of cooking oil are the addition of natural antioxidants, starch wuluh and pineapple pulp. Starfruit contains flavonoids, terpenoids, phenols and pectins as well as Vitamins C and A which can absorb free radicals in oil. Meanwhile, pineapple pulp contains high levels of vitamin C, flavonoids and polyphenols which can eat free radicals. This Research aims to determine the differences in the antioxidant power of starfruit and pineapple pulp to maintain the quality of used cooking oil. This research is experimental, which will study the antioxidant power of starfruit and pineapple pulp at the acid number, peroxidant and color intensity of used cooking oil. 3 grams of dry starfruit and 0.4 grams of dried pineapple pulp are added to each pad 100 mL of used cooking oil. The application of starfruit and pineapple pulp in used cooking oil had a significant effect on the 3 oil quality parameters, namely the acid number, the peroxide number and the oil color intensity, with a significance value of P <0.05. This is because the antioxidant content and composition of the two ingredients are different. Wuluh starfruit in the addition of 3 grams provides a fairly large antioxidant effect, while pineapple pulp at a concentration of 0.4 grams has shown anti-oxidant power so a higher concentration is needed to produce optimal antioxidant power. Keywords: Antioxidants, Starfruit, Pineapple Dregs Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7509 10.30651/jmlt.v4i1.7509 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 88 - 99 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7509/3943 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7554 2022-07-13T05:24:40Z analis:ART

Prevalensi Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA) Pada Peralatan Laboratorium -, kurniawan Kurniawan Tyas, Eva Adaning -, Supriyadi Staphylococcus aureus bacteria are common flora bacteria that are both commensal and opportunistic pathogens in humans. They are commonly treated with antibiotics, which can lead to bacterial resistance, such as the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, which are resistant to the β -lactam antibiotics that are currently widespread to a variety of communities, such as the campus community, which is equipped with several facilities such as laboratories. The microbiology laboratory is a practicum and research laboratory that uses materials in the form of microorganisms, hence it has the potential to transmit MRSA bacteria. The aim of this study is to find out if MRSA bacteria are present on microbiology lab equipment and how prevalent they are. This study used observational research with a descriptive research design and a purposive sampling strategy. The results of bacterial isolation on MSA medium from fourteen laboratory equipment revealed that thirteen devices exhibited bacterial growth, whereas one device had none. Seven bacterial isolates with S. aureus-like features are detected growing in three of the thirteen equipment, the LAF, incubator, and manual autoclave. The seven bacterial isolates were identified as having round colony shape, glistening, opaque, convex yellow pigmentation, round cell morphology, clustered like grapes and purple in color, positive for coagulase and catalase, and resistance to methicillin antibiotics (MRSA bacteria). It can be concluded that of the fourteen-laboratory equipment examined, three instruments, namely the LAF, incubator, and manual autoclave, were found to be overgrown with MRSA bacteria, with the prevalence of MRSA bacteria in microbiology laboratory equipment reaching 21.4%.  Keywords: laboratory equipment, MRSA, prevalence, S. aureus Universitas Muhammadiyah Surabaya 2021-12-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7554 10.30651/jmlt.v4i2.7554 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 188-200 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/7554/4425 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7592 2022-10-31T02:39:03Z analis:ART

Identifikasi dan Enumerasi Escherichia coli dengan Kombinasi Metode MPN-PCR Kurniawati, Atik Listyorini, Dwi Witjoro, Agung Healthy and nutritious food should be free from biological contaminants. The presence of Escherichia coli in food is an indicator contamination and also presence of other pathogenic bacteria. This study aims to determine the value of MPN E. coli in food samples using the MPN-PCR combination technique. Descriptive research method. Samples were taken from 5 traders in Malang City. The results showed that 3 out of 5 food samples were contaminated with E. coli, indicated by the presence of a specific band amplified and produced a DNA band measuring 584 base pairs in agarose gel electrophoresis. Conclusion: MPN value of E. coli in the three food samples code II 34 MPN / gram, code III 75 MPN / gram and V code 290 MPN / gram. The MPN-PCR combination can be used as an alternative method for the conventional MPN test because it can detect the presence of E. coli bacteria in food quickly and reliably Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7592 10.30651/jmlt.v5i1.7592 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 49-55 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7592/5092 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7604 2022-07-13T05:23:37Z analis:ART

ANALISA CEMARAN LOGAM BERAT (Pb, Cd, Zn) PADA MAKANAN DAN MINUMAN KEMASAN KALENG DENGAN MENGGUNAKAN METODE Spektrofotometri Serapan Atom (SSA) Kunsah, Baterun Kartikorini, Nastiti Ariana, Diah AbstractMakanan dan minuman yang dikemas dalam kaleng memiliki kemungkinan untuk terkontaminasi logam berat yang berasal dari komponen penyusun kaleng. Kontaminasi logam berat bisa menyebabkan keracunan dan berbahaya bila masuk ke dalam system metabolism tubuh dengan jumlah yang melebihi batas yang telah ditetapkan dalam SNI dan BPOM. Penelitian ini bertujuan untuk menganalisa Timbal (Pb), Kadmium (Cd) dan  seng (Zn) pada sarden, kornet, susu kaleng, sari buah, minuman berkarbonasi. pengambilan sampel dilakukan di dua lokasi berbeda yaitu pasar modern dan pasar tradisional, dengan tujuan agar dapat membandingkan perbedaan pada pasar modern dan pada pasar tradisional.Serapan logam diukur dengan menggunakan spektofotometri serapan atom (SSA) pada gelombang yang spesifik. Hasil penelitian adalah kadar cadmium (Cd) tidak teridentifikasi, kadar logam Timbal (Pb) pada sampel makanan dan minuman kaleng adalah 0.01 mg/kg, 0.018 mg/kg, 0.026 mg/kg, 0.012 mg/kg, 0.027 mg/kg, 0.027 mg/kg, 0.018 mg/kg, 0.015 mg/kg, 0.024 mg/kg dan 0.019 mg/kg, sementara itu cemaran Zn  adalah 0.188 mg/kg, 0.169 mg/kg, 0.193 mg/kg, 0.184 mg/kg, 0.129 mg/kg, 0.105 mg/kg, 0.107 mg/kg, 0.098 mg/kg, 0.176 mg/kg, 0.152 mg/kg. Semua sampel makanan dan minuman kaleng tidak ada yang melebihi batas maksimum cemaran yang telah di tetap kan dalam SNI dan Badan Pengawas Obat dan Makanan. Kadar cemaran logam berat Pb dan Zn pada sampel makanan dan minuman kaleng dari pasar tradisional lebih tinggi daripada sampel makanan dan minuman kaleng dari pasar modern              Kata kunci : Makanan-Minuman Kemasan Kaleng, SSA, Timbal, kadmium, seng Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7604 10.30651/jmlt.v4i1.7604 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 100 - 110 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7604/3944 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7605 2022-07-13T05:23:37Z analis:ART

PROFIL TANIN PADA TEH SEDUH DENGAN PAPARAN SUHU PENYEDUHAN YANG BERBEDA Ariana, Diah Kartikorini, Nastiti Mardiyah, Siti Abstract   Tea is a beverage widely consumed by all levels of society as in addition to economic and easy to get, tea is also deemed to provide health benefits. One of the compounds that plays a role as antioxidant and antimicrobial in the tea for body health is tannin. The size of tannin content received by body depends on the way of processing tea before drinking. The higher the infusion temperature of tea infusion, it will make the content of tannin in the drinks is increasing.   So the statement of problem in this research is whether there is any effect of infusion temperature on tannin content in tea infusion. This research aims to find out the effect of infusion temperature on tannin content in tea infusion.   The type is experimental research. The sample is infused tea mostly consumed by people which is sold in market of Pacar Keling Surabaya. Total of samples used in this research is 40 grams of infused tea packages divided into two (2) treatments with 8 repetitions in each treatment. The variables are independent variable namely infusion temperature while the dependent variable is tannin content.   From the examination of tannin content based on infusion temperature, it is known that the average of tannin content with infusion temperature of 700C and 1000C is 42.84% and 43.41% in a gram unit or 100 grams of infused water. The result of paired t test shows there is no effect of infusion temperature on tannin content in infusion tea with significant value more than 0.05 or 5%. Possible factor of no influence of temperature on tannin content is the length of infusion used which shall be not too long around 3-5 minutes. Password: Tea, Infusion Temperature, Tannin Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7605 10.30651/jmlt.v4i1.7605 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 111 - 119 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7605/3945 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7672 2022-07-13T05:24:40Z analis:ART

Potensi Metabolit Sekunder Isolat Aktinomycetes Sebagai Penghasil Senyawa Antibakteri Terhadap Methicillin Resistant Staphylococcus aureus (MRSA) Dari Tanah Mangrove Wonorejo Surabaya Anggraini, Anita Dwi Puspitasari, Ayu Rahayuningsih, Christ Kartika Infectious diseases are estimated to be the cause of half of all deaths worldwide. About 50-75% of deaths in hospital are reported to be due to infection. This number continues to increase rapidly in line with the development of bacterial resistance to certain antibiotics. One of the causes of resistance due to antibiotic abuse. The number of resistance events that occur encourages new discoveries in order to overcome the problem of resistance. Actinomycetes are Gram-positive microscopic bacteria that are widespread in nature. These bacteria can live in various environmental conditions that contain lots of nutrients. Actinomycetes population increases in the presence of organic matter that undergoes decomposition. Acinomycetes have the ability to produce antimicrobial compounds. The need for antibiotic compounds and chemotherapy compounds that are effective with low toxicity to the host, and the resulting waste can be degraded by the environment, is a problem that needs serious attention. The existence of resistance to antibiotics causes the need to develop new types of antibiotics from natural ingredients and microorganisms that can control microbapatogens. Compounds obtained from natural materials, especially plants and microbes, provide promising results in the development of new antibiotic compounds, among the existing types of microorganisms, Actinomycetes are the most potential source of producing antibiotics. Apart from antibiotics, actinomycetes also produce bioactive compounds of high economic value in the health sector as antiviral and anti-cancer, while in agriculture as herbicides, insecticides, and anti-parasitic compounds. This causes the need to explore actinomycetes which have the potential to produce antibiotic compounds against bacteria. This study aims to determine the antibacterial activity of secondary metabolites of actinomycetes isolates against cuts infected with Staphylococcus aureus resistant methicillin (MRSA) in winstar rats (rattus norvegicus). This type of research is carried out in an experimental laboratory. The results showed that the potential for secondary metabolites of actinomysetes isolates against MRSA bacteria was classified as strong with an inhibitory index of 10.1. Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7672 10.30651/jmlt.v4i2.7672 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 181-187 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/7672/4423 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7815 2022-07-13T05:24:40Z analis:ART

Analisis Kadar Timbal (Pb) Dan Serum Glutamic Pyruvic Transaminase (SGPT) Pada Pengemudi Ojek Online Di Kota Denpasar, Bali yuan, rahayu artini Aryasa, I Wayan Tanjung Cahyaningrum, Putu Lakustini motorcycle taxi is a transportation service with communication technology in the world. The average mobility of online motorcycle taxi drivers is between 8-12 hours per day, this will certainly affect the physical condition and function of the drivers' organs, especially motorbike drivers. The purpose of this study was to determine the health status of online motorcycle taxi drivers, which was reviewed from liver function tests, namely serum glutamic pyruvic transaminase (SGPT) and exposure to heavy metal lead (Pb). This study used an experimental experimental research design pretest-posttest control group design. In this study, there was an increase in the levels of SPGPT and lead (Pb) for online motorcycle taxi drivers in Denpasar City with the SGPT level for male drivers being 14.14% and for women 19.62% and lead (Pb) levels for male drivers. -Male is 2.61% and female 5.14%. Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7815 10.30651/jmlt.v4i2.7815 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 170-180 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/7815/4408 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7936 2022-07-13T05:24:40Z analis:ART

Uji Kualitas DNA Darah Pada Kertas Whatman Yang Diisolasi Dengan CHELEX-100 Serta Variasi Waktu Penyimpanan Maliza, Rita Pratiwi, Lutfi Sukma Perwitasari, Dyah Aryani DNA extraction from dried blood spots was used for molecular analysis. Dried blood spots sample usually used FTA cards, but short-term use would come at a cost. This study aims to identify DNA extraction quality from dried blood spots in Whatman filter paper as an alternative storage sample. This study used 15 samples with three different storage time for 1, 3, and 7 days on 4°C. Dried blood samples were extracted using the Chelex-100 method, and qualitative were identified by electrophoresis. The DNA extraction was used as a template on PCR amplification of the gapdh gene. The result showed that DNA extraction showed band from 1, 3, and 7 days, and PCR amplification showed bands in 200 bp. In conclusions, DNA from dried blood samples was stored for 1, 3 and 7 days in Whatman filter paper were successfully extracted by the Chelex-100 method and can be used as a DNA template for PCR amplification. Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7936 10.30651/jmlt.v4i2.7936 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 113-119 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/7936/4471 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/7939 2022-10-31T02:39:03Z analis:ART

Efek Obat Antituberkulosis Fase Intensif dan Konsumsi Purin Terhadap Kadar Asam Urat Pasien Tuberkulosis Mustaming, Mustaming ABSTRACT A high prevalence of TB requires serious treatment with Anti-Tuberculosis Therapy, but the side effects can gain uric acid levels. This study aimed to analyze the effects of intensive phase therapy and high purine consumption patterns with uric acid levels in TB patients with Anti-Tuberculosis Therapy. This research was an analytic study using a prospective cohort design. The population was all tuberculosis patients who received Anti-Tuberculosis Therapy at several Public Health Centers in Samarinda. The sampling technique used consecutive sampling approach. The respondents were selected according to the inclusion criteria and had signed the informed consent. Furthermore, the venous blood was measured to know the levels of the uric acid pre-test. The measurements of uric acid levels post-test were carried out after undergoing Anti-Tuberculosis Therapy for 2 months and filling out the FFQ questionnaire to determine the patterns of high purine consumption. The collected data was analyzed using a t-paired and a one-way anova test. T-Paired test results exhibited that there were increasing trend in the average uric acid levels after following Anti-Tuberculosis Therapy in an intensive phase of 2.53 mg / dL (39.78%) with p-value <0.001. Besides, the one way anova test results showed that the average difference in uric acid levels in respondents who consumed high purine 4-6 times/week was 3.16 mg / dL and was higher than respondents who never consumed high purine or those who consumed high purine 1-3 times/week with a p-value <0.001. An average increase in uric acid levels in TB patients after undergoing the intensive phase of Anti-Tuberculosis Therapy for 2 months with a pyrazinamide and ethambutol regimen and an increase in uric acid levels was higher compared to respondents who consumed high purine. Keywords: uric acid level, high purine consumption, intensive phase Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/7939 10.30651/jmlt.v5i1.7939 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 15-23 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/7939/5088 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/8255 2022-07-13T05:23:37Z analis:ART

STABILITAS PEMERIKSAAN HEMATOLOGI RUTIN PADA SAMPEL DARAH YANG DIDIAMKAN PADA SUHU RUANG MENGGUNAKAN CELL-DYN RUBY Nugraha, Gilang Ningsih, Nur Anita Sulifah, Titik Fitria, Sitti Complete blood count (CBC) is one of the laboratory tests most often influenced by doctors. The use of a hematology analyzer offers a wider range of probe parameters. The pre-analytic stage accounts for 70% of errors, one of which is the delay of the examination. Changes in report results were reported due to changes in blood cell morphology due to EDTA additives and room temperature. The aim of this research is the disturbance of stability of the results of examination of various CBC parameters in blood samples that are left at room temperature for 24 hours using a hematology analyzer. This experimental laboratory research was conducted at the Pramita Jemur Andayani Clinical Laboratory. Blood samples were obtained from volunteers, stored at room temperature and subjected to immediate examination (control) and after a delay of 6, 12 and 24 hours (treatment). A total of 30 respondents, consisting of 8 men and 22 women. The mean age of the respondents was 22 ± 1 year. There was no difference in the results of the examination (p-value > 0.05) in the RBC, Hct MCV, MCHC, PLT and PDW. The results of the examination (p-value < 0.05) were found on Hgb, MCH, RDW, WBC, NEU, IG, MONO, EO, BASO, LYM, PLT and PDW. Delayed CBC examinations using the CELL-DYN Ruby hematology analyzer directly gave different results on several parameters ranging from 6 hours delay of examination. Universitas Muhammadiyah Surabaya 2021-06-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/8255 10.30651/jmlt.v4i1.8255 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 21 - 29 2614-2805 2597-3681 10.30651/jmlt.v4i1 ind https://journal.um-surabaya.ac.id/analis/article/view/8255/3936 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/9567 2022-07-13T05:24:40Z analis:ART

Perbedaan Hasil Pemeriksaan Kromium Heksavalen (Cr VI) Dengan Pengompleks 1,5 Difenilkarbazida Pada Waktu 5 Menit Dan 15 Menit Secara Spektrofotometri UV-VIS Sari, Indah Mariadi, Pra Dian Sebayang, Rosnita Differences of hexavalent chromium (Cr VI) examination results with complexing of 1,5 diphenylcarbazide at 5 minutes and 15 minutes by spectrophotometry uv-vis at (Balai Riset dan Standard Industri Palembang) Research and Industry Standard Center Palembang, 2017. Study Program of DIV Health Analyst, Faculty of Health Sciences of Musi Charitas Catholic University Palembang. Chromium (VI) is a carcinogenic component for humans, the amount of chromium that is safe to consume by humans is 50 - 200 μg a day. This research was an analytical comparative, conducted in The Research and Industry Standard Center Palembang. Samples were from pro-analytical chemical content made from chromium-acting susceptibility of 0,1 ppm and 0,2 ppm and the samples were given a complex of 1,5 diphenylcarbazide in the period of 5 minutes and 15 minutes. The obtained chromium examination results with complexing 1,5 diphenylcarbrazide in the period of 5 minutes were 0,0869 and 0,1775 and in the period of 15 minutes were 0,0932 and 0,1626. The data analyzed by using Wilcoxon p value and it was obtained p> 0,05. Based on this research, it was concluded that chromium examination with complexing of 1,5 diphenylcarbrazide in the period of 5 minutes and 15 minutes had no significant difference. Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/9567 10.30651/jmlt.v4i2.9567 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 135-140 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/9567/4396 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/9580 2022-07-13T05:24:40Z analis:ART

Perbedaan Kadar Trigliserida Pada Sampel Darah Segera Disentrifugasi Dan Sampel Darah Dibekukan Selama 20 Menit Sebelum Disentrifugasi Familianti, Reni Junika Sari, Indah Bastian, Bastian Trigliserida atau yang sering disebut triasilgliserol adalah salah satu jenis lemak yang terdapat dalam darah dan berbagai organ tubuh yang dibentuk dari gliserol dan lemak yang ada dalam makanan yang dikonsumsi secara berlebihan. Nilai trigliserida yang tinggi memiliki kaitan dengan timbulnya penyakit serangan jantung, stroke dan diabetes. Penelitian ini bertujuan untuk mengetahui perbedaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan selama 20 menit yang dilakukan di Balai Besar Laboratorium Kesehatan Palembang. Jenis penelitian yang digunakan adalah Cross sectional komparatif, dengan rancangan penelitian intact group comparison. Sampel berupa 30 serum segera disentrifugasi dan 30 serum dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Penelitian ini dilakukan mulai dari persiapan sampel, pengambilan bahan pemeriksaan, pengolahan bahan pemeriksaan, analisis dan hasil penelitian. Kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel serum segera disentrifugasi yaitu 0.826 mmol/L sedangkan kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel dibekukan 20 menit sebelum disentrifugasi yaitu 0.918 mmol/L. hasil uji dependent t test diketahui bahwa nilai signifikan adalah p = 0,342. Nilai p yang didapatkan adalah p > 0,05. Hasil penelitian dapat disimpulkan bahwa tidak ada perbedaan hasil pemeriksaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Kata kunci : Kadar Trigliserida, Segera Sentrifugasi, Dibekukan 20 MenitDaftar Pustaka: 31 (2013-2020) Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/9580 10.30651/jmlt.v4i2.9580 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 120-126 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/9580/4426 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/9691 2022-07-13T05:24:40Z analis:ART

Uji Aktivitas Anti Bakteri Dari Formula Ekstrak Daun Ciplukan (Physalis angulata L.) dan Batang Kayu Manis (Cinnamomum burmannii) Secara In Vitro Test Muzakky, Fuad Alkautsar, Malika Ilma Syawalia, Anni Humayroh Azizi, Nabila Mirza Rullyansyah, Subhan Ciplukan leaves (Physalis angulata L.) and Cinnamon (Cinnamomum burmannii) are one of medicinal plants that contain antibacterial active compounds. This study aims to determine the antibacterial activity of the combination formula between extracts of ciplukan leaves and cinnamon sticks against the growth of Staphylococcus aureus bacteria in Vitro. Ciplukan leaves (Physalis angulata L.) and cinnamon (Cinnamomum burmannii) were extracted by the maceration method with 70% ethanol. Ciplukan leaf extract (Physalis angulata L.) and cinnamon (Cinnamomum burmannii) were tested against Staphylococcus aureus bacteria using the good method by measuring transmittance on a spectrophotometer at a wavelength of 580 nm with 3 repetitions, 100 ppml standard Septromycin solution as control positive and aquadest as a negative dick. Inhibition zone diameter of ciplukan leaf extract (Physalis angulata L.) x̄ = 12.46 mm, Cinnamon Extract (Cinnamomum burmannii) x̄ = 18.93 mm, while in the formulation group x̄ = 22.45 mm. One-way analysis of variance (ANOVA) of these data compared with Streptomycin activity (positive control) showed significant inhibitory activity by the formulated extract against S. aureus. Keywords: Antibacterial, Staphylococcus aureus,  Ciplukan leaves (Physalis angulata L.) and Cinnamon (Cinnamomum burmannii) Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/9691 10.30651/jmlt.v4i2.9691 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 127-134 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/9691/4395 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/9745 2022-07-13T05:24:40Z analis:ART

Pemanfaatan Serum Hemolisis Dengan Penambahan Reagen Anti-Rh Terhadap Kadar Enzim Serum Glutamic Pyruvic Transminase (SGPT) Bastian, Bastian Sari, Indah Sari, Helnia Trianes, Juwy Serum glutamate pyruvate transferase (SGPT) also known as alanine aminotransferase (ALT) is an enzyme found in liver cells and is effective in diagnosing hepatocellular destruction. Examination of SGPT activity using blood serum is often difficult due to insufficient blood volume or lysed serum conditions due to improper collection. This study aims to determine whether there is use of serum hemolysis using anti-rh reagents in the examination of SGPT enzyme levels. This type of research uses an experimental design - static group comparison. The sample will be taken by students of DIV Medical Laboratory Technology IKest Muhammadiyah Palembang. The research was carried out starting from patient preparation, taking examination materials, processing examination materials, analysis and examination results. The results of the ANOVA test showed that the significant value was p = 0.000. The p value obtained was p> 0.005. The results of the study found that there was a significant difference in the amount of SGPT in normal serum and serum with the addition of anti-Rh to hemolysis serum Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/9745 10.30651/jmlt.v4i2.9745 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 141-147 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/9745/4397 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/10393 2022-10-31T02:39:03Z analis:ART

Uji Efektivitas Asap Cair Batang Bambu (Bambusa sp) sebagai Antiseptik Oktavia Pah, Chintia Agatha Batang bambu (Bambusa sp) biasa digunakan dibidang industri,tetapi batang bambu juga dapat diolah sebagai asap cair karena memiliki senyawa alami yaitu fenol, asam asetat dan karbonil yang dapat digunakan sebagai antimikroba. Penelitian ini bertujuan untuk mengurangi bahan kimia dalam penggunaan antiseptik. Dilakukan uji efeltivitas asap cair batang bambu secara in vitro dan in vivo, secara in vitro dilakukan dengan metode kirby bauer, metode ini terdiri dari difusi cakram untuk bakteri dengan jamur, dan metode sumuran untuk jamur, dengan alkohol 70% sebagai kontrol positif dan akuades sebagai kontrol negatif, konsentrasi asap cair yang digunakan adalah 50%, 75%, dan 100% untuk mengetahui konsentrasi optimum dalam menghambat pertumbuhan S.aureus ATCC 25923, E.coli ATCC 25922, C.albicans ATCC 10231 dan A.flavus ATCC 9643. Sementara itu, uji efektivitas secara in vivo dilakukan dengan mengaplikasikan asap cair batang bambu konsentrasi 100% pada telapak tangan responden. Perhitungan jumlah koloni bakteri dan jamur dilakukan sebelum dan setelah diberi perlakuan. Metode yang digunakan adalah Total Plate Count (TPC). Hasil asap cair batang bambu secara in vitro menunjukkan adanya zona hambat pada konsentrasi optimum 100% dengan nilai rata-rata S.aureus ATCC 25923 (6,83 mm), E.coli ATCC 25922 (3,3 mm) sedangkan pada C.albicans ATCC10231 dan A.flavus ATCC 9643 tidak terdapat zona hambat pada konsentrasi optimum 100%. Hasil secara in vivo menunjukkan asap cair batang bambu konsentrasi 100% efektif dalam menghambat pertumbuhan bakteri sebesar 82,3% dan jamur sebesar 73%. Hasil penelitian in vivo didukung dengan hasil kuesioner kesukaan responden terhadap asap cair batang bambu yaitu diantaranya suka terhadap warna 91,6%, terhadap aroma 75%, suka terhadap asap cair karena tidak menimbulkan efek kekeringan pada tangan sebesar 91,7% dan tidak menimbulkan efek lainnya (seperti sensasi terbakar) 100%. Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/10393 10.30651/jmlt.v5i1.10393 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 65-80 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/10393/5094 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/10604 2023-06-05T09:01:26Z analis:ART

The Potential of Averrhoa bilimbi Juice As An Alternative Reagent in Proteinuria Examination Kristinawati, Erna Nur, Nutri Farda hasanah, idyatul Background: Proteinuria indicates a kidney disorder and provides important prognostic information in diagnosing kidney disease. To determine the presence of protein in the urine, a urinalysis examination is carried out.  Proteinuria examination generally uses the heating method with acetic acid, sulfosalicylic acid, and concentrated nitric acid as reagents. In Indonesia, the use of acetic acid for proteinuria examination is still the primary choice. An alternative reagent is needed that can replace the function of acetic acid. The objective of this study is to determine the potential of Averrhoa Blimbi juice as an alternative reagent for proteinuria examination.Methods: This was a Pre-experimental study with a completely randomized design. This study used urine samples from a patient with chronic kidney disease. Urine samples were examined using 6% acetic acid reagent as a control and using Averrhoa bilimbi juice with various concentrations. Each juice concentration was examined twice so that the experimental units in this study were 40 units. The Kruskal-Wallis test was used to determine the potential of Averrhoa bilimbi  as an alternative reagent.Results: At concentrations of 5%-20% showed the same positive results as 6% acetic acid, namely +2, concentration 25%-35% resulted in +3, and other concentration resulted in +4. The results of data analysis showed p=0.04 (p<0.05). This shows that Averrhoa bilimbi has the potential as an alternative reagent for proteinuria examination.Conclusion: Averrhoa bilimbi  juice with a 5%-20% concentration has the potential as an alternative reagent for 6% acetic acid to check proteinuria levels.Keywords: Averrhoa bilimbi ; alternative reagents; proteinuria; acetic acid; urinalysis Universitas Muhammadiyah Surabaya 2023-05-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/10604 10.30651/jmlt.v6i1.10604 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist; 30-36 2614-2805 2597-3681 10.30651/jmlt.v6i1 ind https://journal.um-surabaya.ac.id/analis/article/view/10604/6362 Hak Cipta (c) 2023 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/10623 2022-12-26T04:45:15Z analis:ART

Perbandingan Kadar C-Reactive Protein Terhadap Fase Pemberian Obat Anti Tuberkulosis Seno, Amalia Ovi Mustika Wardani, Dita Pratiwi Kusuma Supriyadi, Supriyadi Mulyanto, Arif Pulmonary tuberculosis is a disease caused by Mycobacterium tuberculosis. C-Reactive Protein (CRP) is produced in the body by the liver in response to inflammation caused by Mycobacterium tuberculosis infection. CRP as the main mediator of the non-specific immune system, and the concentration will increase to 1000 times higher than normal value in the event of tissue injury or infection. The aim of this study was to determine the comparison of CRP levels to the phase of anti-tuberculosis drug administration in pulmonary tuberculosis patients. The study was conducted by cross sectional study with a semi-quantitative method in June - July 2020 in Purwokerto Community Lung Health Center. The number of samples as much as 24 TB patients which is 12 TB patients in 0 month and 12 TB patients in 6 months. Data were analyzed by Mann Whitney Test. The mean rank in 0 month was 18.08 with median (min-max) 12 (12-24) mg / dL, whereas mean rank for 6 months was 6.92 with median (mix-max) was 0 (0- 12) mg / dL. Serum CRP levels in pulmonary TB patients in the 0-month treatment phase are higher than CRP levels in the 6-month treatment phase (p = 0,000). Universitas Muhammadiyah Surabaya 2022-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/10623 10.30651/jmlt.v5i2.10623 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 115 - 123 2614-2805 2597-3681 10.30651/jmlt.v5i2 ind https://journal.um-surabaya.ac.id/analis/article/view/10623/5636 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/10656 2022-10-31T02:39:03Z analis:ART

Efektifitas Nutrisi Hidroponik AB MIX Sebagai Larvasida Alternatif Larva Nyamuk Aedes aegypti Lukiyono, Yauwan Tobing Rohmayani, Vella - ABSTRACT            Hydroponics is a technique for cultivating vegetable and fruit plants. The principle of hydroponic technique is the provision of nutrient solution as a source of plant nutrients. These plants are grown in certain media using water instead of soil. The presence of stagnant water in hydroponic techniques can be a breeding place for Aedes aegypti larvae. Aedes aegypti mosquito can do as a vector for dengue fever. Dengue fever is still a public health problem today. The purpose of this study was to analyze the potential of AB mix as a nutrient for hydroponic plants as larvicides of the Aedes aegypti mosquito.This research was a pure experimental research. The research design used a pre post only group design. The research sample was F3 larvae of Aedes aegypti instar III. The research variables were the mortality of Aedes aegypti larvae and variations in the concentration of AB mix. The test was repeated 10 times. Data were analyzed using one-way ANOVA. Based on the results obtained in all treatments there was death or mortality of mosquito larvae. The administration of 75% AB Mix with a duration of 70 minutes is the most optimal treatment to eradicate Aedes aegypti mosquito larvae. So, it can be concluded that AB Mix can be used as an alternative larvicide to eradicate Aedes aegypti mosquito larvae. Keywords                   : AB Mix, mortalitas dan Larva Aedes aegypti Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/10656 10.30651/jmlt.v5i1.10656 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 24-28 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/10656/5089 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/10870 2022-10-31T02:39:03Z analis:ART

Pembuatan Membran Antiseptik Dari Kombinasi Kitosan Dan Kulit Jeruk Nipis (Citrus aurantiifolia Biomi, Agnes Ayu yuan, rahayu artini As technology develops, air quality tends to decline. One of the air pollutants that is often encountered is bacteria that infect through the air such as Staphylococcus aureus. As a basic protection tool, a cloth mask with a membrane layer can be an alternative solution. One of the materials that can be used is chitosan and lime peel pectin. Lime peel produces essential oils that are used as stimulant, aromatherapy, and antimicrobial enhancers. This study aims to determine the results of characteristic tests, content analysis through phytochemicals and Staphylococcus aureus. The research method used is descriptive exploratory and experimental. The membrane mask made from chitosan of shrimp skin and lime peel has a characteristic thickness of 00.24 mm with a density of 0.60 - 0.90 g/cm3 with a textured heterogeneous surface. Has a water vapor transmission power of 0.00088 g/day/m2. It takes 27.54 days to completely decompose. Grapefruit peel essential oil contains secondary metabolites in the form of alkaloids, flavonoids, tannins, phenolics and triterpenoids. The membrane mask had Staphylococcus aureus antibacterial activity ranging from 14.05 mm. Keywords: Lime peel (Citrus aurantiifolia), Shrimp Skin Chitosan, Membrane mask, Antibacterial. Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/10870 10.30651/jmlt.v5i1.10870 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 56-64 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/10870/5093 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:ojs.journal.um-surabaya.ac.id:article/10871 2021-11-18T09:06:23Z analis:ART

oai:journal.um-surabaya.ac.id:article/10872 2022-07-13T04:25:08Z analis:ART

GAMBARAN PEMERIKSAAN KADAR GLUKOSA DAN KOLESTEROL PADA LANSIA Purwaningsih, Nur Vita Azizah, Fitrotin Maulidiyanti, Ellies Tunjung Sari Artanti, Dita The increasing life expectancy based on WHO indicates an increase in the number of elderly people (elderly) so that there is an increase in health problems in the elderly due to the aging process which causes many changes in the body of the elderly. One of the changes in the body of the elderly is the blood glucose and cholesterol regulation system, resulting in an increase of more than normal. The purpose of this study was to determine the profile of glucose and cholesterol levels in the elderly. This research is observational. The population and sample in this study were the elderly in Karang Penang. The examination method used in this examination is the POCT (Point Of Care Test) examination method. The results showed that the elderly who had levels as much as 10 respondents (20%) samples had normal glucose levels, while as many as 40 respondents (80%) samples had high glucose. For cholesterol levels, 20 respondents (40%) samples had normal cholesterol levels, while 13 respondents (26%) samples had threshold cholesterol levels, then 7 respondents (14%) samples had high cholesterol levels. Keywords        : blood glucose, cholesterol and the elderly Universitas Muhammadiyah Surabaya 2019-07-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/10872 10.30651/jmlt.v2i2.10872 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 2 No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist; 65 - 71 2614-2805 2597-3681 10.30651/jmlt.v2i2 ind https://journal.um-surabaya.ac.id/analis/article/view/10872/4559 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11015 2022-10-31T02:39:03Z analis:ART

Daya Hambat Ekstrak Daun Sirih dan Daun Legundi Terhadap Pertumbuhan Bakteri Staphylococcus aureus Suyasa, Ida Bagus Oka Bekti, Heri Setiyo Rinawati, Luh Putu Laksmi, Luh Putu Wahyuni, Putu Diah Agustini, Desak Gede Dwi Rakhmawati, Aprilia Staphylococcus aureus is one of the causes of infectious disease, ranging from mild to severe infections such as meningitis. The drug that is often used is the penicillin class of antibiotics. Apart from chemical antibiotics, natural antibacterial compunds from plants can be also used as an alternative treatment for this infection. Such as green betel (Piper betle L.) and legundi (Vitex trifolia L.). The ethanol extract of betel leaf has antibacterial activity against Staphylococcus aureus. The phytochemical content in legundi leaf is also able to inhibit the growth of Staphylococcus aureus. The ethanol extract of betel leaf and legundi is made through a maceration process. The extract concentrations used in this study were 20%, 30%, and 40%. The inhibition test of Staphylococcus aureus using the disc method. From the results of this study, the active substances found in betel leaf were flavonoids, tannins, and phenols, while in legundi were found flavonoids, tannins and phenols, and quinones. In total, the mean inhibition zone in this study ranged from 7.87mm to 17.33mm where legundi leaf extract was in the moderate category, betel and combination extract were classified as strong. There was a difference in the diameter of the inhibition zone of betel and legundi extract at a concentration 20% with 40%. In the combination extract there is a difference in the concentration of 20% with 40% and 30% with 40%. An antimicrobial compound can be said to synergize if a mixture of two or more antimicrobial compounds is able to provide an effect of each compounds. In this study, it has seen that the mean of the combination is higher than the mean of legundi leaf extract. Meanwhile, the mean of betel leaf extract is still greater than the combination extract. Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11015 10.30651/jmlt.v5i1.11015 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 29-41 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/11015/5090 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11130 2022-07-13T05:24:40Z analis:ART

Pemantauan Pasien Dengan Diagnosa Gagal Ginjal Kronik Di RSUD Sumber Rejo Bojonegoro Samsudin, Rinza Rahmawati Widyastuti, Rahma Purwaningsih, Nur Vita The kidneys are important organs that function to maintain blood composition by preventing the accumulation of waste and controlling the balance of fluids in the body. Chronic Kidney Disease (CKD) has become a major health problem of Indonesian people. Creatinine examination become one of the choices to identify the index of the kidney severity. The purpose of this study was to determine the description of creatinine levels of patients diagnosed with Chronic Kidney Disease (CKD). This research used descriptive method. The population of this study was patients with the diagnosis of Chronic Kidney Disease (CKD) who conduct the examination of creatinine levels in the installation of Pathology ClinicLaboratory which amounted to 100 patients during April to May. This research used the secondary data obtained and collected from the LIS (Laboratory Information System) and the medical record. The result of this study showed tht 73% of 100 patients diagnosed with Chronic Kidney Disease (CKD) are not normal, and the 27% data of creatinine levels is normal. Presentations of male patient who experienced levels of creatinine was high, namely 37 patients compared female. The incidence of patients with Chronic Kidney Disease (CKD) the highest range of productive age of 20-64 years was 50 patients (68%). The results of the research of the average content on patients diagnosed with Chronic Kidney Disease (CKD) obtained the creatinine level average of 4.01 mg/dl.   Keywords: Chronic Kidney Disease (CKD), Creatinine Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11130 10.30651/jmlt.v4i2.11130 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 148-156 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/11130/4398 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11135 2022-07-13T05:24:40Z analis:ART

Studi Pemeriksaan Sedimen Urine berdasarkan Karakteristik Pada Penikmat Kopi di Asrama Kiwal Brawijaya Surabaya Wiliyanarti, Pipit Festy Atrasina, Jilan Maulidiyanti, Ellies Tunjung Sari Coffee is a psychostimulant drink which can provide good benefits for the body if consumed in moderation. However, one of the effects in excessive coffee consumption over a long period of time that is not accompanied by adequate fluid intake is in fact able to affect sedimentation in urine. The caffeine content which has a lot of particles can cause the kidneys to be unable to reabsorb it. Therefore, it is excreted along with the urine and it causes the urine to be more concentrated. This causes increased sedimentation in the urine. The purpose determined the description of urine sediment examination on coffee connoisseurs in Asrama Kiwal in Brawijaya of Surabaya. The research type used descriptive research with a research sample of 40 people taken by purposive sampling. The results showed that the description of urine sediment examination on coffee connoisseurs at Asrama Kiwal in Brawijaya of Surabaya that 12 people (30%) had normal urine sediment and 28 people (70%) had abnormal urine sediment. Based on the characteristics of the respondents, the presence of abnormal urine sediment was found in the age category 30 years as many as 26 people (76%) with a frequency of coffee consumption > 3 times per day as many as 14 people (35%) in a series of coffee consumption for > 10 years as many as 27 people ( 67.5%) and the frequency of consumption of mineral water 2 litters per day as many as 24 people (60%). The conclusion of this study was that most of the coffee connoisseurs in Asrama Kiwal Brawijaya of Surabaya that experienced abnormal urine sedimentation.Keywords: Coffee, Urine Sediment Universitas Muhammadiyah Surabaya 2021-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11135 10.30651/jmlt.v4i2.11135 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 157-169 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/11135/4399 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11151 2022-07-13T05:24:40Z analis:ART

Formulasi Dan Karakteristik Fisik Sediaan Plester Hidrogel Ekstrak Daun Ciplukan (Physalis angulata L.) Dan Batang Kayu Manis (Cinnamomum burmannii) Hanistya, Ria Samlan, Karima Diabetes mellitus is a degenerative disease caused by the damage of beta pancreatic cells. This caused many complication such as diabetic ulcer which left untreated can cause an open wound hard to heal thereby reducing the quality of life. Treatment of diabetic ulcer despite using antidiabetic also using an antibiotic therapy like Mupirocin. In its development, the use of natural ingredients is known to be used as adjuvant therapy for diabetic ulcer such as Ciplukan leaves and Kayu Manis bark. Both of the plant contain phytochemical compound like sinamaldehid, tannin, polyphenol and flavonoid which can work as wound healer. To increase its efficacy then Ciplukan leaves and Kayu Manis bark were formulated as hydrogel patch. Hydrogel patch are known has advantages like better penetration, hygienic and easy to administered Ciplukan (Physalis angulata L.) leaves and Kayu Manis (Cinnamomum burmannii) Bark extracted with maceration technique. The extracts then made into matrix type hydrogel patch with 10% HPMC and 5% PVA as backing patch. The backing patch and extract then placed on a square cast and dried under room temperature for 24 hours. The hydrogel patch then evaluated for physical characteristic including organoleptic aspect, weight uniformity, moisture content and pH. The results showed that Ciplukan leaves and Kayu Manis Bark hydrogel patch have a semi solid consistency, transparent greenish color and distinctive smell. The average pH is 5,5 and have average weight around 1,18 grams. The moisture contens of hydrogel patch is 2,42%. The Ciplukan leaves and Kayu Manis bark is able to formulated into hydrogel patch. The hydrogel patch is showing good appearance and having good properties as topical dosage form. The pH of the hydrogel patch is suitable with pH of the skin and have no intention to irritate the skin. Hydrogel patch also giving a cooling sensations in wound area thus increase patients convenience and acceptability.  Keywords: Diabetes mellitus, extract, drug delivery system, hydrogel patch, adjuvant therapy Universitas Muhammadiyah Surabaya 2021-12-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11151 10.30651/jmlt.v4i2.11151 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 201-208 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/11151/4429 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11280 2022-10-31T02:39:03Z analis:ART

Evaluasi Penggunaan Biosaliva Dalam Deteksi Sars-Cov-2 Metode RT-PCR Kesuma, Suryanata Makkadafi, Suparno Putera SARS CoV-2 infection, which has affected the world since late 2019, can cause serious lower respiratory tract infections that may be fatal in some patients. This infection causes the disease Covid-19. The diagnosis of SARS-CoV-2 infection was carried out by NAAT (Nucleic Acid Amplification Test) such as RT-PCR examination. The sample needed for the identification of SARS-COV-2 is a nasopharyngeal/oropharyngeal swab. Nasopharyngeal/oropharyngeal swab sampling requires trained personnel. Taking a nasopharyngeal/oropharyngeal swab is invasive, causing discomfort in its implementation. The convenience of sampling specimens can be an alternative option for the identification of SARS-CoV-2, such as with newly developed biosaliva specimens. The use of this biosaliva sample can be a practical option in the examination of the identification of SARS-CoV-2. However, the use of these specimens needs to be evaluated first because of the possible relationship with clinical findings and so that the results of the SARS-CoV-2 examination are valid and reliable. The purpose of this study was to evaluate the use of biosaliva specimens to detect SARS-CoV-2 infection with the RT-PCR method. Evaluation of the use of biosaliva in the detection of SARS-COV-2 RT-PCR method with paired T test and diagnostic test with the gold standard using nasopharyngeal/oropharyngeal swabs. The target genes for the detection of SARS-CoV-2 are the RdRp gene and the E gene with control of the HRP gene. RT-PCR was carried out with 40 cycles and Tm 62 °C. The results of this study are Sig. (2-tailed) paired T test was 0.106, sensitivity was 64.86% and specificity was 90.92%. The conclusion of this study is that there is no statistical difference in the results of the SARS-CoV-2 RT-PCR method between the use of biosaliva specimens and nasopharyngeal/oropharyngeal swabs, and the evaluation results show that reliable biosaliva specimens are used as samples in the examination of SARS-COV-2 infection. Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11280 10.30651/jmlt.v5i1.11280 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 42-48 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/11280/5091 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11282 2022-07-13T05:24:40Z analis:ART

Uji Efektifitas Perasan Jahe (Zingiber officinale) Terhadap Pertumbuhan Jamur Candida albicans Azizah, Fitrotin Artanti, Dita Candidiasis is one of the most common infectious diseases caused by Candida sp. In 2017 the Mycology Division of Dr. Soetomo said there were 67 patients diagnosed with candidiasis. The use of natural ingredients as an alternative to traditional medicine is increasing because it is safe for consumption and does not cause side effects. One of the natural ingredients is Ginger (Zingiber officinale). The choice of using ginger juice (Zingiber officinale) is because the ingredients are easy to obtain and easy to apply by the community. This study aims to determine the antifungal effectiveness of ginger (Zingiber officinale) juice against the growth of Candida albicans. This research is experimental by doing the treatment which was repeated 4 times, namely the concentration of ginger juice 20%, 40%, 60%, 80%, 100%, negative control (aquadest) and positive control (ketoconazole). The inhibition zone examination method used the Kirby Bauer diffusion method which was characterized by the formation of a clear zone around the paper disc on MHA (Muller Hinton Agar) media. The inhibition zone formed was measured using a caliper, then the data obtained were analyzed using the Mann-Whitney test. From laboratory examination, it was found that the average diameter of inhibition formed with concentrations of 20%, 40%, 60%, 80%, and 100% was 0 mm, and the negative control (aquadest) was 0 mm. The results of statistical analysis showed that 0.05 obtained a significant value between the administration of juice concentrations of 20%, 40%, 60%, 80%, 100% and not given (negative control-aquadest) = 1,000. From these results, it was concluded that there was no significant difference between the concentrated juice of 20%, 40%, 60%, 80%, 100% and not given (negative control - distilled water)Keywords : Ginger (Zingiber officinale), Candida albicans Universitas Muhammadiyah Surabaya 2021-12-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11282 10.30651/jmlt.v4i2.11282 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 209-215 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/11282/4499 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11523 2022-07-13T05:24:40Z analis:ART

Efektivitas Lama Perendaman Serbuk Kulit Jeruk Manis (Citrus sinensis) Terhadap Bilangan Peroksida Pada Minyak Jelantah Kartikorini, Nastiti Kunsah, Baterun Ariana, Diah Used cooking oil is the remaining cooking oil from the frying process. The use of cooking oil repeatedly at high temperatures and for a long time causes the peroxide number to increase. High peroxide levels can present a health hazard. Phenolic and flavonoid content contained in sweet orange peel is an antioxidant that acts as an antidote to free radicals so that it can slow down the oxidation of used cooking oil. The purpose of this study was to determine the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. This type of research is experimental with the number of sample repetitions for each treatment, namely 25 samples. The formulation of the problem of this research is whether there is an effect of the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. From the results of the examination, the average levels of peroxide numbers in used cooking oil that had been soaked were 1 day 22.7949 mEq, 2 days 19.5965 mEq, 3 days 13.5975 mEq and 4 days 9.5984 mEq. Statistical analysis using ANOVA showed the effect of the effect of soaking duration of sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil p (sig) = 0,000 which is less than 0.05.  Keywords:  Sweet Orange, Peroxide Number, Used Cooking Oil.  Universitas Muhammadiyah Surabaya 2021-12-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11523 10.30651/jmlt.v4i2.11523 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 4 No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist; 216-224 2614-2805 2597-3681 10.30651/jmlt.v4i2 ind https://journal.um-surabaya.ac.id/analis/article/view/11523/4526 Hak Cipta (c) 2021 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/11932 2023-06-05T09:01:26Z analis:ART

Pengaruh Air Rebusan Daun Sirsak (Annona muricata Linn) Terhadap Kadar Asam Urat Pada Wanita Usia Lebih Dari 45 Tahun Di Desa Besuki Kecamatan Lumbir Kabupaten Banyumas Sulistiyowati, Retno Kusumaningrum, Agny Asam urat merupakan senyawa kimia hasil akhir dari metabolisme purin dalam tubuh. Wanita mengalami hiperurisemia meningkat pada wanita setelah menopause, karena mengalami penurunan hormon estrogen. Daun sirsak mengandung flavonoid yang bersifat sebagai antioksidan yang dapat mengurangi terbentuknya asam urat. Tujuan penelitian menganalisis pengaruh pemberian air rebusan daun sirsak terhadap kadar asam urat pada wanita usia di atas 45 tahun di Desa Besuki. Penelitian berjenis kuantitatif dengan menggunakan metode pre-experimental design tipe one group pretest – posttest. 14 responden mengkonsumsi air rebusan daun sirsak sebanyak satu gelas sehari selama 2 minggu. Hasil penelitian menunjukkan penurunan kadar asam urat setelah pemberian air rebusan daun sirsak, dengan rerata kadar asam urat sebelum diberi perlakuan 6,0714±1,06946 mg/dL sedangkan rerata kadar asam urat setelah diberi perlakuan 5,4071±1,36971 mg/dL. Rerata nilai perbedaan kadar asam urat sebelum dan sesudah diberi perlakuan sebesar 0,6429±0,72707 mg/dL. Universitas Muhammadiyah Surabaya 2023-05-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/11932 10.30651/jmlt.v6i1.11932 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist; 52-58 2614-2805 2597-3681 10.30651/jmlt.v6i1 ind https://journal.um-surabaya.ac.id/analis/article/view/11932/6407 Hak Cipta (c) 2023 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/12148 2022-12-26T04:45:15Z analis:ART

Analisis Sensitivitas Dan Spesifisitas COVID-19 Ag Rapid Test Terhadap PCR Test Murti, Wisnu Wijayanti, Dian Rachma Safari, Wulan Fitriani SARS-Cov-2 is the newest coronavirus caused Covid-19 which can infect human and pandemic, COVID-19 parameter testing can be done with serological tests used to detect antibody or antigen and also molecular test using PCR method. This research is aimed to compare the sensitivity and specificity between COVID-19 Ag rapid test and PCR test. This research is an analytical descriptive study with 421 samples were obtained from documentation study with probability systematic random sampling and by using secondary data taken from Laboratory Information System (LIS) at Siloam Hospitals Lippo Village Tangerang and the type of observational analytical research with cross sectional design. The analysis results show the sensitivity rate was 96.64%, specificity was 93.75%, PPV was 89.44%, NPV was 98.08%, LR (+) was 15.46, and LR (-) was 0.04. The conclusion of the research result, COVID-19 Ag rapid test is valid to be used as a diagnostic test because it has high rate of sensitivity and specificity according to WHO regulations.    Universitas Muhammadiyah Surabaya 2022-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/12148 10.30651/jmlt.v5i2.12148 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 132 - 138 2614-2805 2597-3681 10.30651/jmlt.v5i2 ind https://journal.um-surabaya.ac.id/analis/article/view/12148/5638 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/12441 2023-06-05T09:01:26Z analis:ART

Sensitivitas Pemeriksaan Covid-19: Insulated Isothermal PCR (iiPCR) Dan Reverse Transcription PCR (RT-PCR) Yunita, Lisa Wijayanti, Dian Rachma Riyanti, Apriani Covid-19 (Corona Virus disease 2019) is a new type of disease caused by a virus from the Corona Virus group, namely SARS-CoV-2. Covid-19 may cause respiratory system disorders, ranging from mild symptoms such as flu to lung infections, such as pneumonia. Laboratory diagnoses for Covid-19 disease generally include hematological (complete blood examinations), and molecular or a combination of serology and molecular. PCR examination that can be carried out using the Insulated Isothermal PCR and Reverse Transcription PCR methods. This research is descriptive observational research. Data were collected at Grha Kedoya Hospital, West Jakarta, North Kedoya. The result showed a sensitivity of iiPCR score 82,6%, which is relatively lower than gold-standard RT-PCR. Our research suggested that RT-PCR is still an effective and sensitive method for Covid-19 examination. Universitas Muhammadiyah Surabaya 2023-05-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/12441 10.30651/jmlt.v6i1.12441 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist; 37-42 2614-2805 2597-3681 10.30651/jmlt.v6i1 ind https://journal.um-surabaya.ac.id/analis/article/view/12441/6359 Hak Cipta (c) 2023 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/12667 2022-10-31T02:39:03Z analis:ART

Stabilitas Sampel Darah Terhadap Profil Hematologi Dengan Metode Otomatis Puspitasari, puspitasari Aliviameita, Andika Sample stability, part of the pre-analytic phase is an important component that can affect clinical laboratory results. Handling of blood samples and storage methods can significantly affect the results of hematological examinations. This study aims to determine the effect of delay time and storage temperature on the results of the examination of leukocyte count, hemoglobin level, erythrocyte count, hematocrit level, and platelet count. The type of research used is a quantitative analysis using laboratory experimental methods. The research was carried out in the hematology laboratory of the Medical Laboratory Technology Study Program, University Muhammadiyah of Sidoarjo in September - December 2021. The results of the Two Way ANOVA test showed that there was no statistically significant effect between the length of time delay and storage temperature on the results of the examination of leukocyte count, hemoglobin level, number of erythrocytes. Friedman test results showed that there was a statistically significant effect between the length of time of delay and storage temperature on hematocrit levels and platelet counts. Universitas Muhammadiyah Surabaya 2022-06-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/12667 10.30651/jmlt.v5i1.12667 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 1-7 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/12667/5086 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/12824 2022-10-31T02:39:03Z analis:ART

Prevalensi Hasil Uji Saring Hbsag Pada Darah Donor Di Unit Tranfusi Darah (UTD) PMI Sampang Madura Widyastuti, Rahma Purwaningsih, Nur Vita Maulidiyanti, Ellies Tunjung Sari Saputro, Tri Ade The screening blood test is a stage that is carried out by each PMI to ensure the safety before blood transfusion. It is usually done on antibodies (Ab) such as anti-HCV, anti-HIV, TPHA or antigen (Ag) such as HBsAg. HBsAg is the outer coat protein of hepatitis B virus (HBV) and a sign that the individual has been infected with the hepatitis B virus. Hepatitis B virus can cause acute problems and even cirrhosis of the liver. One of the screening blood test methods which is used to detect hepatitis B is ELISA (Enzyme Linked Immuno Sorbent Assay) method. This research was conducted at UTD PMI Sampang by using the data collection in February 2020, and aimed to find out the overview of HBsAg examination results on the screening blood test using ELISA method based on age and sex. Based on the screening blood test, 9 people (3%) were reactive HBsAg and 345 people (97%) were non-reactive. In the age category, the highest number of reactive HBsAg donors was in the age group of 18-24 years old, that was 4 donors (1.1%), while the highest number of non-reactive HBsAg donors was in the age group of 25-44 years old, that was 184 people ( 51.9%). In the gender category, donors with the most reactive HBsAg results were male, that was 8 people (2.3%) and donors with the highest non-reactive HBsAg results was male, that was 283 people (79.9%). In order to minimize hepatitis B, it is expected that society should avoid activities which can cause hepatitis B infection. Keywords        : HBsAg, Blood Transfusion, Screening Blood Test  Universitas Muhammadiyah Surabaya 2022-06-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/12824 10.30651/jmlt.v5i1.12824 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 81-90 2614-2805 2597-3681 10.30651/jmlt.v5i1 ind https://journal.um-surabaya.ac.id/analis/article/view/12824/5146 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/13350 2022-12-26T04:45:15Z analis:ART

Perbedaan Kadar C-Reaktive Protein (CRP) Pada Sampel Serum dan Plasma K3EDTA Dengan Metode Imunoturbidimetri Helniasari, Helniasari Nurhidayanti, Nurhidayanti Bastian, Bastian Examination of levels of C-Reactive Protein (CRP) in the blood is one of the tests. that can detect inflammation at an early stage. C-Reactive Protein (CRP) is an inflammatory marker that is synthesized in the liver. CRP levels increase due to trauma, bacterial infection and inflammation (inflammation and tissue damage). CRP is synthesized by the liver and It released into the bloodstream for 6-10 hours after the acute inflammatory process and acute destruction. Tissue detection CRP is also one of several proteins often referred to as the acute phase and It is used to monitor changes in the acute inflammatory phase associated with many infectious and autoimmune diseases. Examination of C-Reactive Protein (CRP) used serum and plasma samples of K3EDTA. C-Reactive Protein levels were measured using the Immunoturbidimetric method. This type of research was cross sectional. The study was conducted at the Clinical Pathology Laboratory, Faculty of Science and Technology, Institute of Health Sciences and Technology Muhammadiyah Palembang on December 21, 2021 with samples were 30. The results of the study had an average value in serum samples of 6.3 mg/L and an average value in plasma. K3EDTA 6.5 mg/L. Wilcoxon test results obtained p value = 0.000. The conclusion of the study was that there were differences in the examination of C-Reactive Protein (CRP) levels in serum and plasma samples of K3EDTA using the immunoturbidimetric method. Universitas Muhammadiyah Surabaya 2022-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/13350 10.30651/jmlt.v5i2.13350 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 139 - 148 2614-2805 2597-3681 10.30651/jmlt.v5i2 ind https://journal.um-surabaya.ac.id/analis/article/view/13350/5639 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/13478 2022-12-26T04:45:15Z analis:ART

Efektifitas Ekstrak Lidah Mertua (Sansevieria trifasciata var laurentii) Sebagai Antifungi Candida albicans Paramastri, Prasasti Kusumaning Qurrohman, Muhammad Taufiq Candidiasis is a disease caused by Candida albicans, this disease has a mortality rate of > 25%, mainly affecting adults, with a frequency of women 2 - 3 times more than men (Raningsih et al., 2018). A commonly used fungi drug is ketoconazole. but it has many side effects so it is necessary to find for alternatives to natural ingredients, namely the leaf of lidah mertua (Sansevieria trifasciata var laurentii) because it contains compounds saponins, flavonoids, triterpenoids, and steroids as antifungals The leaf of lidah mertua were extracted by maceration technique. The purpose of this research was to determine the effect of antifungi of ethanol extract the leaf of Lidah Mertua (Sansiviera trifasciata var Laurentii) against the fungus Candida albicans. This research was experimental using the disc diffusion method with a spread plate technique in the inhibition zone formed at concentrations of 25%, 20%, 15%, 10%, 5%, positive control and negative control. The results of the One way Anova test is known that differ significantly. From the results of this research, it can be concluded that the ethanol extract of the leaves of the leaf of lidah mertua (Sansevieria trifasciata var laurentii) has an effect on the antifungal activity of Candida albicans Keywords       : Candida albicans, Leaf of Lidah Mertua, Antifungal, Ketokonazole Universitas Muhammadiyah Surabaya 2022-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/13478 10.30651/jmlt.v5i2.13478 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 149 - 158 2614-2805 2597-3681 10.30651/jmlt.v5i2 ind https://journal.um-surabaya.ac.id/analis/article/view/13478/5640 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/14103 2022-12-26T04:45:15Z analis:ART

Antibakteri Cuka Apel Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Secara In Vitro Aryasa, I Wayan Tanjung Artini, Ni Putu Rahayu Vinegar has the chemical name acetic acid derived from the Latin word acetum, acetic acid, ethanoic acid, or vinegar is an organic acid chemical compound that is the most important carboxylic acid in commerce, industry, and laboratories known as a sour taster. and aroma in food. But keep in mind that vinegar can work as a disinfectant. One type of vinegar that we know is apple cider vinegar. Based on the background that has been stated above, the problem formulated in this study is how apple cider vinegar inhibits the growth of Escherichia coli and Staphylococcus aureus bacteria. The analysis of this research is in the form of a quasi-experimental, namely by comparing the inhibitory power of apple cider vinegar and control on the growth of Escherichia coli and Staphylococcus aureus bacteria and post-test only control group design. Based on the results of the research that has been carried out, the results show that the diameter of the inhibition zone formed on Escherichia coli bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar is 12.33 ± 0.12 mm, 2.21 ± 0.12 mm and 7.42 ± 0.09 mm. While the diameter of the inhibition zone formed on Staphylococcus aureus bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar was 14.56 ± 0.46 mm, 0.00 ± 0.00 mm, and 10.36 ± 0.27 mm. So it can be concluded that apple cider vinegar has antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria. Universitas Muhammadiyah Surabaya 2022-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/14103 10.30651/jmlt.v5i2.14103 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 106 - 114 2614-2805 2597-3681 10.30651/jmlt.v5i2 ind https://journal.um-surabaya.ac.id/analis/article/view/14103/5635 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

oai:journal.um-surabaya.ac.id:article/14261 2022-12-26T04:45:15Z analis:ART

Potensi Daun Kelor (Moringa oleifera) Terhadap Kadar Kadmium Dan Kreatinin Dalam Darah Sebagai Indikator Kerusakan Fungsi Ginjal Pada Tikus Putih Yang Terpapar Asap Rokok Rahayuningsih, Christ Kartika Agustin, Riya Idayanti, Tacik High exposure of cigarettes in Indonesia causes various disturbances in the body's metabolism, especially in the kidney, which is due to the cadmium metal content. Cadmium induces oxidative stress by the formation of free radicals and decrease natural antioxidant activity in the kidney organs (nephrotoxic). One way to overcome nephrotoxic by decreasing blood creatinine, we can utilize Moringa oleifera (MO) which is known as a "miracle plant" and have high contents of flavonoid groups, phenols, gallic acid, ascorbic acid and carotenoids as antioxidants and natural metal chelating agents. This research aimed to analyze the potential of Moringa leaves in decreasing cadmium and creatinine levels in the blood of white rats due to exposure of cigarette smoke (inhalation) at doses 200, 400 and 800 mg/kgwb. The type of research was an experimental study with quantitative analysis conducted in October 2021 – July 2022. The independent variable was the doses of Moringa leaves extract and the dependent variable were the blood cadmium and creatinine levels of white rats. The blood cadmium level was measured with AAS and the creatinine level was measured with Jaffe method without deproteination by photometry. The results of blood cadmium and creatinine levels in the treatment of MO extract at dose 200 mg/kgwb were 1.288875 μg/L and 0.85 mg/dL; MO extract at dose of 400 mg/kgwb were 0.811 μg/L and 0.775 mg/dL; MO extract at dose 800 mg /kgwb were 0.86275 μg/L and 0.7 mg/dL. Based on statistical analysis using Kruskal wallis, the results of the p value on cadmium levels in the blood were 0.004 < 0.05 and the p value on creatinine levels was 0.003 < 0.05, showed that there were significant differences in blood cadmium and creatinine levels in the treatment groups. Moringa leaf extract (Moringa oleifera) at a dose of 400 mg/kgwb had good potential in overcoming kidney damage in terms of creatinine levels due to cadmium exposure through cigarette smoke, which was equivalent to 44.3756 grams of fresh Moringa leaves that can be implemented for human as an additional antioxidant for overcoming free radicals.                                                       Keywords : Moringa oleifera, Cigarettes Smoke, Cadmium, Creatinine, Rattus norvegicus Universitas Muhammadiyah Surabaya 2022-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artikel Peer-review application/pdf https://journal.um-surabaya.ac.id/analis/article/view/14261 10.30651/jmlt.v5i2.14261 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST; Vol 5 No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist; 159 - 173 2614-2805 2597-3681 10.30651/jmlt.v5i2 ind https://journal.um-surabaya.ac.id/analis/article/view/14261/5644 Hak Cipta (c) 2022 THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST 9e01da229be955b01909e23460f620c1