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Diabetes mellitus is a metabolic disorder characterized by not produced insulin or disruption of insulin action. Diabetes mellitus was reported correlated with TCF7L2 gene mutation. This study aimed to analyze the TCF7L2 gene in families with type 2 diabetes. 5 samples were used in this study, the methods flow through DNA Isolation, PCR, and sequencing. Samples were amplified by the PCR technique using primers that were designed to detect the TCF7L2 gene, (F: 5'-GGCTTGATTGTTGATTATGGGC3' and R:5 'TCTGGCACTCAGAGAGT 3'). The amplification and sequencing resulted in 368 bp (located at 103631-103972 bp). We found a transition mutation at C103950T. The polymorphisms found may characteristic in this population because they are not found in other sequences.


Keywords : Diabetes mellitus, TCF7L2 gene, mutation

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  1. Suneja, S, Christian, Y, & Chandra, N 2018, ‘Milieu of Diabetes in the 2 nd Decade of 21 st Century’, Journal Of Diabetes Of Metabolism, vol. 9, no. 9, hh. 1–14.
  2. Petersson, C, & Andersson, K 2010,’Standars of medical care in diabetes,’diabetic retirophaty vol. 19, no. 40, hh. 1-6.
  3. Riaz, S, 2009, ‘Diabetes mellitus,’Scientific Research and Essay, vol. 4, no. 5, hh. 367–373.
  4. Shafee, T, & Lowe, R 2017, ‘Eukaryotic and prokaryotic gene structure,’WikiJournal ofMedicine, vol. 4 no. 1, hh. 2.
  5. ‘Welcome trust case control consortium 2007, ‘Genome-wide association study of 14,000 cases of seven commom diseasses and 3,000 shared controls,’Nature europe PMC funders, vol. 6, no. 447, hh. 661-678. doi:10.1038/nature05911.
  6. Beloso, C, Souto, J, & Mimbacas, A 2018, ‘Association of TCF7L2 mutation and atypical diabetes in a uruguayan population,’World Journal Of Diabetes, vol. 9, no. 9, hh. 157-164. DOI: 10.4239/wjd.v9.i9.157.
  7. Jyothi, U, & Mohan, B 2011, ‘Genetic etiology of type 2 diabetes mellitus,’Research Society For Study Of Diabetes In India, vol. 1, no. 6, hh. 3-10. DOI 10.1007/s13410-011-0020-8.
  8. Vural, H 2017, ‘gene polimorphism in T2 dm with patient in turkish population,’journal of clinical epigetics vol. 3, hh. 2-27. doi: 10.211767/2472-1158.100061.
  9. Yang, Q 2011, ‘Harrison's Endocrinology,’In The Yale Journal of Biology and medicine, vol. 84, no. 4, hh. 497-498. /PMC3238314.
  10. Franca, C & Kist, T 2002, ‘Areview of DNA seguencing techniques,’Queaterly Review Of Biophzsics vol. 35, hh. 169-200.
  11. Ridwan, M 2018, ‘elektroforesis analisis elektronika terhadap biokimia genetika,’jurnal ilmiah pendidikan tehnik elektro, vol. 2 no. 1, hh. 21-26.
  12. Rahn, K, Clarke, C & Curtiss, R 2012, ‘amplifikasi urutan gen invA Salmonella thypitrium oleh reaksi berantai polimerase sebagai metode spesifik deteksi,’Salmonella. Probe molekul dan seluler,vol. 6,hh. 271-279.
  13. Delianis, P 2010, ‘Karakter senyawa bioaktif bakteri simbion moluska dengan GC-M,’Jurnal dan ilmu teknologi kelautan, vol. 2, no. 2, hh. 34-40.
  14. Syamsuril, H 2019,’genotyping SPN rs 793146 TCF7L2 gene for detection T2DMT in indonesia melayu ethnis,’Journal of shyscs vol. 13, no. 17, hh. 1-6. DOI: 10.1088/1742-6596