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Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge.

 

Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.

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This work is licensed under  Creative Commons Attribution-NonCommercial 4.0 International License.

 

Biografi Penulis

Mulya Fitrah Juniawan, UNIVERSITAS MUHAMMADIYAH SURABAYA

PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS MUHAMMADIYAH SURABAYA

Dita Artanti, UNIVERSITAS MUHAMMADIYAH SURABAYA

MIKROBIOLOGI PRODI D3 TEKNOLOGI LABORATORIUM MEDIS FAKULTAS ILMU KESEHATAN UNIVERSITAS MUHAMMADIYAH SURABAYA

Yuni Gayatri, UNIVERSITAS MUHAMMADIYAH SURABAYA

PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS MUHAMMADIYAH SURABAYA

AINUTAJRIANI AINUTAJRIANI, UNIVERSITAS MUHAMMADIYAH SURABAYA

TEKNOLOGI LABORATORIUM MEDIS, FAKULTAS ILMU KESEHATAN, UNIVERSITAS MUHAMMADIYAH SURABAYA

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