Potensi Daun Kelor (Moringa oleifera) Terhadap Kadar Kadmium Dan Kreatinin Dalam Darah Sebagai Indikator Kerusakan Fungsi Ginjal Pada Tikus Putih Yang Terpapar Asap Rokok

Christ Kartika Rahayuningsih, Riya Agustin, Tacik Idayanti


High exposure of cigarettes in Indonesia causes various disturbances in the body's metabolism, especially in the kidney, which is due to the cadmium metal content. Cadmium induces oxidative stress by the formation of free radicals and decrease natural antioxidant activity in the kidney organs (nephrotoxic). One way to overcome nephrotoxic by decreasing blood creatinine, we can utilize Moringa oleifera (MO) which is known as a "miracle plant" and have high contents of flavonoid groups, phenols, gallic acid, ascorbic acid and carotenoids as antioxidants and natural metal chelating agents. This research aimed to analyze the potential of Moringa leaves in decreasing cadmium and creatinine levels in the blood of white rats due to exposure of cigarette smoke (inhalation) at doses 200, 400 and 800 mg/kgwb. The type of research was an experimental study with quantitative analysis conducted in October 2021 – July 2022. The independent variable was the doses of Moringa leaves extract and the dependent variable were the blood cadmium and creatinine levels of white rats. The blood cadmium level was measured with AAS and the creatinine level was measured with Jaffe method without deproteination by photometry. The results of blood cadmium and creatinine levels in the treatment of MO extract at dose 200 mg/kgwb were 1.288875 μg/L and 0.85 mg/dL; MO extract at dose of 400 mg/kgwb were 0.811 μg/L and 0.775 mg/dL; MO extract at dose 800 mg /kgwb were 0.86275 μg/L and 0.7 mg/dL. Based on statistical analysis using Kruskal wallis, the results of the p value on cadmium levels in the blood were 0.004 < 0.05 and the p value on creatinine levels was 0.003 < 0.05, showed that there were significant differences in blood cadmium and creatinine levels in the treatment groups. Moringa leaf extract (Moringa oleifera) at a dose of 400 mg/kgwb had good potential in overcoming kidney damage in terms of creatinine levels due to cadmium exposure through cigarette smoke, which was equivalent to 44.3756 grams of fresh Moringa leaves that can be implemented for human as an additional antioxidant for overcoming free radicals.                                                       Keywords : Moringa oleifera, Cigarettes Smoke, Cadmium, Creatinine, Rattus norvegicus

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Abdel-Daim, M. M. et al. (2020) ‘Alleviation of lead acetate-induced nephrotoxicity by Moringa oleifera extract in rats: highlighting the antioxidant, anti-inflammatory, and anti-apoptotic activities’, Environmental Science and Pollution Research, 27(27). doi: 10.1007/s11356-020-09643-x.

Abou-Zeid, S. M. et al. (2021) ‘Moringa oleifera ethanolic extract attenuates tilmicosin-induced renal damage in male rats via suppression of oxidative stress, inflammatory injury, and intermediate filament proteins mRNA expression’, Biomedicine and Pharmacotherapy, 133. doi: 10.1016/j.biopha.2020.110997.

Adeyemi, O. S. and Elebiyo, T. C. (2014) ‘Moringa oleifera Supplemented Diets Prevented Nickel-Induced Nephrotoxicity in Wistar Rats’, Journal of Nutrition and Metabolism, 2014. doi: 10.1155/2014/958621.

Akinrinde, A. S. et al. (2020) ‘Nephroprotective effect of methanol extract of moringa oleifera leaves on acute kidney injury induced by ischemia-reperfusion in rats’, African Health Sciences, 20(3). doi: 10.4314/ahs.v20i3.44.

Depkes (2017) InfoDATIN Pusat Data dan Informasi Kementerian Kesehatan RI: Situasi Penyakit Ginjal Kronis. Jakarta: Pusat Data dan Informasi Kementerian Kesehatan RI.

Fitriana, M. et al. (2020) ‘Karakteristik Fisika Sediaan Suspensi Ekstrak Etanol Daun Gaharu (Aquilaria microcarpa Baill.) dengan Variasi Carboxymethyl Cellulose Sodium (CMC-Na)’, Jurnal Pharmascience, 7(1). doi: 10.20527/jps.v7i1.8087.

Hartini, E. (2018) ‘Toksisitas Logam Berat’, in Pengantar Toksikologi. 1st edn. Semarang: Universitas Dian Nuswantoro, pp. 70–89.

Hill, N. R. et al. (2016) ‘Global Prevalence of Chronic Kidney Disease – A Systematic Review and Meta-Analysis’, CKD Global Prevalence, pp. 1–18.

Hussain, T. et al. (2019) ‘The plant flavonoid, fisetin alleviates cigarette smoke-induced oxidative stress, and inflammation in Wistar rat lungs’, Journal of Food Biochemistry, 43(8). doi: 10.1111/jfbc.12962.

Kerdsomboon, K., Chumsawat, W. and Auesukaree, C. (2021) ‘Effects of Moringa oleifera leaf extracts and its bioactive compound gallic acid on reducing toxicities of heavy metals and metalloid in Saccharomyces cerevisiae’, Chemosphere, 270. doi: 10.1016/j.chemosphere.2020.128659.

Kumar, S. and Pandey, A. K. (2013) ‘Phenolic content, reducing power and membrane protective activities of Solanum xanthocarpum root extracts’, Vegetos, 26(1). doi: 10.5958/j.2229-4473.26.1.043.

Lee, W. K. and Thévenod, F. (2020) ‘Cell organelles as targets of mammalian cadmium toxicity’, Archives of Toxicology. doi: 10.1007/s00204-020-02692-8.

Mayaserli, D. P. and Rahayu, J. S. (2018) ‘Perbandingan Kadar Logam Kadmium ( Cd ) Dalam Urin Perokok’, 5(1), pp. 58–64.

Moraes, C. A. de, Breda-Stella, M. and Carvalho, C. A. F. (2021) ‘Morphofunctional study on the effects of passive smoking in kidneys of rats’, Einstein (Sao Paulo, Brazil), 19. doi: 10.31744/einstein_journal/2021AO6000.

Ratliff, B. B. et al. (2016) ‘Oxidant mechanisms in renal injury and disease’, Antioxidants and Redox Signaling. doi: 10.1089/ars.2016.6665.

Raza, H., John, A. and Nemmar, A. (2013) ‘Short-term effects of nose-only cigarette smoke exposure on glutathione redox homeostasis, cytochrome P450 1A1/2 and respiratory enzyme activities in mice tissues’, Cellular Physiology and Biochemistry, 31(4–5). doi: 10.1159/000350087.

Rosita, B. and Andriyati, F. (2019) ‘PERBANDINGAN KADAR LOGAM KADMIUM (Cd) DALAM DARAH PEROKOK AKTIF DAN PASIF DI TERMINAL BUS’, Sainstek : Jurnal Sains dan Teknologi, 11(2). doi: 10.31958/js.v11i2.1576.

Verdiansah (2016) ‘Pemeriksaan Fungsi Ginjal’, CDK-237, 43(2), pp. 148–154.

Wang, Y. et al. (2017) ‘Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity’, Food Chemistry, 218. doi: 10.1016/j.foodchem.2016.09.058.

Winata, S. D. (2016) ‘Monitoring, Pencegahan, dan Penanganan Keracunan pada Pekerja Terpapar Cadmium’, Jurnal Kesehatan Lingkungan Indonesia, 13(2), pp. 45–50.

Young, J. L. et al. (2019) ‘Cadmium and High-Fat Diet Disrupt Renal, Cardiac and Hepatic Essential Metals’, Scientific Reports, 9(1). doi: 10.1038/s41598-019-50771-3.

DOI: http://dx.doi.org/10.30651/jmlt.v5i2.14261


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