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Staphylococcus aureus bacteria are common flora bacteria that are both commensal and opportunistic pathogens in humans. They are commonly treated with antibiotics, which can lead to bacterial resistance, such as the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, which are resistant to the β -lactam antibiotics that are currently widespread to a variety of communities, such as the campus community, which is equipped with several facilities such as laboratories. The microbiology laboratory is a practicum and research laboratory that uses materials in the form of microorganisms, hence it has the potential to transmit MRSA bacteria. The aim of this study is to find out if MRSA bacteria are present on microbiology lab equipment and how prevalent they are. This study used observational research with a descriptive research design and a purposive sampling strategy. The results of bacterial isolation on MSA medium from fourteen laboratory equipment revealed that thirteen devices exhibited bacterial growth, whereas one device had none. Seven bacterial isolates with S. aureus-like features are detected growing in three of the thirteen equipment, the LAF, incubator, and manual autoclave. The seven bacterial isolates were identified as having round colony shape, glistening, opaque, convex yellow pigmentation, round cell morphology, clustered like grapes and purple in color, positive for coagulase and catalase, and resistance to methicillin antibiotics (MRSA bacteria). It can be concluded that of the fourteen-laboratory equipment examined, three instruments, namely the LAF, incubator, and manual autoclave, were found to be overgrown with MRSA bacteria, with the prevalence of MRSA bacteria in microbiology laboratory equipment reaching 21.4%.

 

 

Keywords: laboratory equipment, MRSA, prevalence, S. aureus

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This work is licensed under  Creative Commons Attribution-NonCommercial 4.0 International License.

 

Biografi Penulis

kurniawan Kurniawan -, Prodi TLM D4 UMP Purwokerto

Prodi Teknologi Laboratorium Medik D4 Universitas Muhammadiyah Purwokerto

Eva Adaning Tyas, Universitas Muhammadiyah Purwokerto

Prodi Teknologi Laboratorium Medik D4 Universitas Muhammadiyah Purwokerto

Supriyadi -, Universitas Muhammadiyah Purwokerto

Program Studi Ilmu Keperawatan S1 Fakultas Ilmu Kesehatan

Referensi

  1. Afifurrahman, A., Samadin, K.H. and Aziz, S., 2014. Pola Kepekaan Bakteri Staphylococcus aureus terhadap Antibiotik Vancomycin di RSUP Dr. Mohammad Hoesin Palembang. Majalah Kedokteran Sriwijaya, 46(4), pp.266-270.
  2. Amalia, A., Sari, I. and Nursanty, R., 2018. Aktivitas Antibakteri Ekstrak Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC.) Terhadap Pertumbuhan Bakteri Methicillin Resistant Staphylococcus aureus (MRSA). Prosiding Biotik, 5(1).
  3. Arjyal, C., Kc, J. and Neupane, S., 2020. Prevalence of methicillin-resistant Staphylococcus aureus in shrines. International journal of microbiology, pp.1-10.
  4. Badan Standarisasi Nasional. 2011. Cara uji mikrobiologi - Bagian 9 : Penentuan Staphylococcus aureus pada produk perikanan SNI 2332.9:2011. Jakarta.
  5. Beri, K., 2018. Skin microbiome & host immunity: applications in regenerative cosmetics & transdermal drug delivery. Future science OA, 4(6), p.FSO302.
  6. Dewi, A.K., 2013. Isolasi, identifikasi dan uji sensitivitas Staphylococcus aureus terhadap amoxicillin dari sampel susu kambing peranakan ettawa (PE) penderita mastitis di wilayah Girimulyo, Kulonprogo, Yogyakarta. Jurnal Sain Veteriner, 31(2), pp.138-150.
  7. Gunawan, I., 2019. Managemen Pengelolaan Alat dan Bahan di Laboratorium Mikrobiologi. Jurnal Pengelolaan Laboratorium Pendidikan, 1(1), pp.19-25.
  8. Herrani, C.R., 2015. Penggunaan Virtual Lab untuk Meningkatkan Keterampilan mahasiswa pendidikan biologi dalam menggunakan alat-alat mikrobiologi. Widya Dharma: Jurnal Kependidikan, 27(2), pp.160-174.
  9. Jayanthi, A.A.I., Tarini, N.M.A. and Praharsini, I.G.A.A., 2020. Staphylococcus aureus sebagai agen penyebab infeksi pada kasus erisipelas kruris dekstra dengan liken simpleks kronikus. Intisari Sains Medis, 11(3):1482-1491
  10. Karimela, E.J., Ijong, F.G. and Dien, H.A., 2017. Karakteristik Staphylococcus aureus yang di isolasi dari ikan asap pinekuhe hasil olahan tradisional Kabupaten Sangihe. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(1), pp.188-198.
  11. Kong, H.H. and Segre, J.A., 2012. Skin microbiome: looking back to move forward. Journal of Investigative Dermatology, 132(3), pp.933-939.
  12. Kurniawan, Ratnaningtyas, N.I., 2018. Efektivitas Ekstrak Kapang Endofit Isolat BR-S1 (A) Terhadap Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA). Meditory: The Journal of Medical Laboratory, 6(2), pp.99-107.
  13. Kurniawan, K., Despita, W.R. and Sudarsono, T.A., 2021. Studi Komparasi Kualitas Bakteriologis Udara Pada Laboratorium Terpadu Universitas Muhammadiyah Purwokerto. Quagga: Jurnal Pendidikan dan Biologi, 13(2).pp 31-36
  14. Kurniawan, K. and Yulistiani, M., 2020. A Production and Activity Test of Anti-Bacterial Compounds of Endophytic Fungi Br-S1 (A) Isolate Extract in Different General Growth Media. Current Trends in Biotechnology & Pharmacy, 14(5), pp.206-212
  15. Novitasari, T.M., Rohmi, R. and Inayati, N., 2019. Potensi Ikan Teri Jengki (Stolephorus indicus) Sebagai Bahan Media Alternatif untuk Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Analis Medika Biosains (JAMBS), 6(1), pp.1-15.
  16. Pangalila F.J.V., Soepandi P.Z., Albandjar C.A., Sukesih, L. dan Enty. 2019. Pedoman Antibiotik Empirik di Unit Rawat Intensif. Jakarta: PERDICI
  17. Rahmawati, R., Apriliana, E. and Agus, A., 2018. Identifikasi Staphylococcus aureus pada Daging Ayam yang Dijual di Pasar Besar Kota Palangka Raya. Borneo Journal of Medical Laboratory Technology, 1(1), pp.13-16.
  18. Rasheed, N.A. and Hussein, N.R., 2021. Staphylococcus aureus: An Overview of Discovery, Characteristics, Epidemiology, Virulence Factors and Antimicrobial Sensitivity. European Journal of Molecular & Clinical Medicine, 8(3), pp.1160-1183.
  19. Ren, Q., Liao, G., Wu, Z., Lv, J. and Chen, W., 2020. Prevalence and characterization of Staphylococcus aureus isolates from subclinical bovine mastitis in southern Xinjiang, China. Journal of dairy science, 103(4), pp.3368-3380.
  20. Riedel, S., Morse, S.A., Mietzner, T.A. and Miller, S., 2019. Jawetz Melnick & Adelbergs Medical Microbiology 28 E. McGraw Hill Professional.
  21. Satari, M.H., 2012. Multidrugs Resistance (MDR) Bakteri Terhadap Antibiotik. FKG Universitas Padjajaran, pp.1-7.
  22. Sutriani, 2018. Pengujian Bakteri Jenis Staphylococcus aureus Pada Ikan Layang Segar (Decapterus Sp). Tugas Akhir. Program Studi Teknologi Pengolahan Hasil Perikanan Politeknik Pertanian Negeri Pangkep.
  23. Toelle, N.N., 2014. Identifikasi dan Karakteristik Staphylococcus sp. dan Streptococcus sp. dari Infeksi Ovarium Pada Ayam Petelur Komersial (Identification and Characteristics of Staphylococcus Sp. and Streptococcus Sp. Infection of Ovary in Commercial Layers). Jurnal Ilmu Ternak Universitas Padjadjaran, 14(1).
  24. Utomo, S.B., Fujiyanti, M., Lestari, W.P. and Mulyani, S., 2018. Uji Aktivitas Antibakteri Senyawa C-4 Metoksifenilkaliks [4] Resorsinarena Termodifikasi Hexadecyltrimethylammonium-Bromide Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli. Jurnal Kimia dan Pendidikan Kimia, 3(3), pp.109-209.