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Kasus Covid-19 telah menurun, namun tetap ada permintaan pemeriksaan Covid-19 dalam jumlah kecil. Metode ekstraksi RNA SARS CoV-2 telah banyak dikembangkan, mulai dari yang bersifat manual hingga otomatisasi. Metode otomatisasi lebih cepat dan efisien karena dapat melakukan ekstraksi banyak sampel per sekali pemeriksaan, namun biaya operasional alat cukup mahal. Jumlah permintaan pemeriksaan yang tidak banyak menyebabkan metode otomatisasi ini tidak efisien dalam hal biaya, sehingga perlu ditempuh usaha alternatif menggunakan metode manual. Perbedaan metode ekstraksi dapat memengaruhi konsentrasi RNA, sehingga pemilihan metode ekstraksi RNA yang tepat menjadi penting supaya didapatkan diagnosis yang akurat. Penelitian ini bertujuan untuk membandingkan konsentrasi RNA SARS CoV-2 dari hasil ekstraksi menggunakan metode spin column manual dan magnetic beads otomatis. Jenis penelitian ini adalah analitis numerik tidak berpasangan yang dilaksanakan di GSI Lab pada bulan April sampai Juni 2024. Penelitian ini menggunakan 30 sampel positif Covid-19 yang dilakukan ekstraksi dengan metode spin column manual dan magnetic beads otomatis. Hasil penelitian ini diuji dengan Mann Whitney. Konsentrasi RNA pada metode ekstraksi spin column manual dan magnetic beads otomatis berturut-turut adalah 3,9 (1,68 – 36,17) ng/µL dan 7,96  (2,28 – 113,59) ng/µL. Hasil uji Mann Whitney adalah 0,00 (nilai p< 0,05), yang berarti terdapat perbedaan yang bermakna antara hasil ekstraksi RNA dengan metode spin column manual dan magnetic beads otomatis. Keterbatasan penelitian ini adalah tidak mengkonfirmasi hasilnya dengan real time PCR.

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