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Tobacco consumption in Indonesia is a health epidemic that has direct or indirect toxicity due to exposure of cigarette smoke which contains harmful substances, especially cadmium metal. Cadmium’s exposure can increase the risk of coronary heart disease due to increased LDL cholesterol. One of the cadmium toxicity treatment by utilizing Moringa oleifera leaves as a natural antioxidant considering the flavonoids, saponins, ascorbic acid, carotenoids, phenolics, interquinones as cadmium chelating agents and β-sitosterol content. Therefore, it was necessary to determine the effectiveness of Moringa leaves extract in lowering blood cadmium and LDL cholesterol levels as indicators of atherosclerosis in white rats exposed to cigarettes smoke. The type of research was an experiment with quantitative analysis, conducted at the nutrition laboratory of the Faculty of Public Health Airlangga University and the clinical chemistry laboratory of Surabaya Health Polytechnic in March-July 2023. The independent variable was the doses of Moringa leaf extracts, while the dependent variables were blood cadmium and LDL cholesterol levels of white rats. The analysis of blood cadmium levels used an atomic absorption spectrophotometer and used precipitation methods by a photometer for LDL cholesterol levels. Based on statistical analysis using one way anova showed that blood cadmium levels had a significant value of < 0.05, followed by post hoc tests it was found that treatment group 2 had significant differences from normal groups. Moringa leaves extract dose of 1500 mg/kgbw has been effective in lowering cadmium and LDL cholesterol levels against the risk of atherosclerosis for 14 days.

Keywords : Atomic Absorption Spectrophotometry, Cadmium, LDL Cholesterol, Moringa oleifera, photometer, Rattus norvegicus

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This work is licensed under  Creative Commons Attribution-NonCommercial 4.0 International License.

 

Biografi Penulis

Christ Kartika Rahayuningsih, Poltekkes Kemenkes Surabaya

Prodi TLM Diploma 3 Jurusan Teknologi Laboratorium Medis

Riya Agustin, Poltekkes Kemenkes Surabaya

Jurusan Teknologi Laboratorium Medis

Referensi

  1. Bais, S., Singh, G. S. and Sharma, R. (2014) ‘ Antiobesity and Hypolipidemic Activity of Moringa oleifera Leaves against High Fat Diet-Induced Obesity in Rats ’, Advances in Biology, 2014. doi: 10.1155/2014/162914.
  2. Ismail, N. A. et al. (2022) ‘Electronic and Conventional Cigarette Exposure Aggravate Metabolic Parameters in High-Fat Diet-Induced Rats’, Open Access Macedonian Journal of Medical Sciences, 10(A). doi: 10.3889/oamjms.2022.9723.
  3. Keli, J. et al. (2013) ‘Cadmium exposure induces vascular injury due to endothelial oxidative stress: The role of local angiotensin II and COX-2’, Free Radical Biology and Medicine, 65, pp. 838–848. doi: 10.1016/j.freeradbiomed.2013.08.167.
  4. Kerdsomboon, K., Chumsawat, W. and Auesukaree, C. (2021) ‘Effects of Moringa oleifera leaf extracts and its bioactive compound gallic acid on reducing toxicities of heavy metals and metalloid in Saccharomyces cerevisiae’, Chemosphere, 270. doi: 10.1016/j.chemosphere.2020.128659.
  5. Konno, K. et al. (2014) ‘Visible, Safe and certain endotracheal intubation using endoscope system and inhalation anesthesia for rats’, Journal of Veterinary Medical Science, 76(10). doi: 10.1292/jvms.14-0146.
  6. Kurutas, E. B. (2016) ‘The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: Current state’, Nutrition Journal. doi: 10.1186/s12937-016-0186-5.
  7. Maurovich-Horvat, P. et al. (2014) ‘Comprehensive plaque assessment by coronary CT angiography’, Nature Reviews Cardiology. doi: 10.1038/nrcardio.2014.60.
  8. Nabilla, V. A. et al. (2022) ‘The Effectiveness Of Moringa Leaf Extract (Moringa oleifera) on Cadmium and LDL Cholesterol Levels in Blood as Indicators of Atherosclerosis in Cadmium (Cd) Induced White Rats (Rattus norvegicus)’, Jurnal Teknokes, 15(3). doi: 10.35882/teknokes.v15i3.268.
  9. Sithu, S. D. et al. (2017) ‘Atherogenesis and metabolic dysregulation in LDL receptor–knockout rats’, JCI Insight, 2(9). doi: 10.1172/jci.insight.86442.
  10. Temitayo Olayemi, A., Jones Olanrewaju, M. and Christopher Oloruntoba, A. (2016) ‘Toxicological evaluation of Moringa oleifera Lam seeds and leaves in Wistar rats’, Pharmacognosy Communications, 6(2). doi: 10.5530/pc.2016.2.8.
  11. Vergara-Jimenez, M., Almatrafi, M. M. and Fernandez, M. L. (2017) ‘Bioactive components in Moringa oleifera leaves protect against chronic disease’, Antioxidants. doi: 10.3390/antiox6040091.
  12. Wang, Y. et al. (2017) ‘Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity’, Food Chemistry, 218. doi: 10.1016/j.foodchem.2016.09.058.
  13. Wardani, D. N. K. (2017) ‘Pengaruh Ekstrak Etanol Daun Kelor (Moringa Oleifera Lam.) Terhadap Jumlah Sel Mast Pada Mencit(Mus Musculus) Model Endometriosis’, Jurnal Biosains Pascasarjana, 19(3), p. 260. doi: 10.20473/jbp.v19i3.2017.260-267.
  14. Yamaguchi, Y. et al. (2007) ‘Oxidants in the gas phase of cigarette smoke pass through the lung alveolar wall and raise systemic oxidative stress’, Journal of Pharmacological Sciences, 103(3). doi: 10.1254/jphs.FP0061055.
  15. Young, J. L. et al. (2019) ‘Cadmium and High-Fat Diet Disrupt Renal, Cardiac and Hepatic Essential Metals’, Scientific Reports, 9(1). doi: 10.1038/s41598-019-50771-3.
  16. ZN, J. (2018) ‘Detoxificating effects of moringa oleifera leaf and zingiber officinale root powder on cadmium toxicity in blood and fur of wistar rats’, Open Access Journal of Translational Medicine & Research, 2(1). doi: 10.15406/oajtmr.2018.02.00030.