Bilah Samping Artikel

Telah diserahkan
November 19, 2020
Diterima
June 19, 2021
Diterbitkan
June 19, 2021
Unduh
PDF
Statistik
Baca Penghitung : 1495 Unduh : 3543

Isi Artikel Utama

Abstrak

Entering the era of globalization, manual tools in clinical laboratories have been replaced by full automatic devices. One of them is the Diatron Abacus Hematology analyzer 3. A relatively new hematology analyzer is required for analytical evaluation. Analytical evaluation is an evaluation of Diatron Abacus 3 on Sysmex KX 21 as a standard in RSUD I.A Moeis Samarinda, and it is very important to do this to assess the performance of the tool. Analytical evaluation is done by determining the value of accuracy, precision, and total error and linearity of measurement results from routine hematological examination parameters, which are erythrocytes, leukocytes, platelets, hemoglobin, and hematocrit. To determine the results of the hematology analyzer evaluation of Abacus 3 Diatron Analyzers on Routine Hematology Parameters in the Hematology Laboratory of the Health Ministry of Health, East Kalimantan. This type of research is observational descriptive, using a total sampling technique, and a sample of 40 complete K3EDTA blood specimens. Data processing using Microsoft Excel and SPSS 20 applications, analyzed using descriptive statistics. The observations were still included in the criteria for acceptance, accuracy or inaccuracy (d%) in erythrocytes 1.8%, leukocytes 8.0%, platelets -5.3%, hemoglobin 2.3% and hematocrit -1.7%; Precision or impression (CV%) in erythrocytes 4.2%, leukocytes 11.1%, platelets 6%, hemoglobin 3.9% and hematocrit 4.5%; Total errors in erythrocytes were 8.7%, leukocytes 17.9%, platelets 23.6%, hemoglobin 8.8% and hematocrit 9.1; Linearity of the measurement results against the routine hematological cell count values performed using Abacus 3 and Sysmex KX 21 has a positive relationship. Acceptance values are still included in the LOA on all parameters examined and still meet the criteria; the accuracy/bias value is smaller than the true value of the parameter being examined, except for leukocytes, which is greater; the precision on the five parameters is greater than the CV% Abacus 3 insert kit; The total error obtained by the TE value is greater than the TEA in the parameters examined, except for smaller platelets; Linearity of the measurement results against the calculated hematology cell routine values performed using Abacus 3 and Sysmex KX 21 have a positive relationship, meaning an increase in measurement values using Abacus 3 is followed by an increase in measurement values using Sysmex KX 21.

Rincian Artikel

Lisensi

 

This work is licensed under  Creative Commons Attribution-NonCommercial 4.0 International License.

 

Biografi Penulis

Suryanata Kesuma, Poltekkes Kemenkes Kalimantan Timur

Prodi DIII Teknologi Laboratorium Medis

Referensi

  1. Abacus 3, P. O. (n.d.). Hematology analyzer Abacus 3. Diapro Health Care Supplies.
  2. Ayuningtyas, Y. (2018). Profil Pemantapan Mutu Alat Hematlogy Analyzer Abbott Ruby Di Instalasi Patologi Klinik RSUD.DR.Moerwadi Di Surakarta. In Tugas Akhir. Setia Budi Surakarta.
  3. Bland, J. M., & Altman, D. G. (2018). Statistical Methods For Assessing Agreement Between Two Methods Of Clinical Measurement. The Lancet, 62(7), 307–310. https://doi.org/10.1128/AAC.00483-18
  4. Cinthia, A., Sukeksi, A., & Santoso, B. (2018). Perbedaan Morfologi Eritrosit Pada Spesimen Darah K3edta Yang Segera Diperiksa Dan Ditunda Selama 3 Jam [Muhammadiyah Semarang]. http://repository.unimus.ac.id/3121/1/Manuscript.pdf
  5. Darmadi, & Permatasari, D. (2018). Perbedaan Jumlah Leukosit Darah Edta Diperiksa Segera Dan Ditunda 2 Jam. Klinikal Sains, 6(2), 30–36.
  6. Departemen Kesehatan RI. (2008). Pedoman Praktik Laboratorium yang Benar(Good Laboratory Practice).
  7. Direktorat Jenderal Bina Pelayanan Medik. https://doi.org/10.11648/j.ijbse.20130102.11
  8. Giavarina, D. (2015). Understanding Bland Altman Analysis. Biochemia Medica, 25(2), 141–151. https://doi.org/10.11613/BM.2013.003
  9. Kadir. (2017). Statistik Terapan:Konsep, Contoh dan Analisis Data dengan Program SPSS/ Lisrel dalam Penelitian (3rd ed.). Cet 4. Depok: Rajawali Pers.
  10. Lasmilatu, M. V. (2019). Perbedaan Hitung Jumlah Trombosit Segera Diperiksa Dengan Jumlah Trombosit Setelah Ditunda 15 Menit, 30 Menit, 45 Menit Dan 60 Menit Pada Darah Edta Karya. Poltekkes Kemenkes Kupang.
  11. Little, R. R., Lenters-Westra, E., Rohlfing, C. L., & Slingerland, R. (2011). Gross Overestimation of Total Allowable Error Based on Biological Variation. Clinical Chemistry, 57(9), 1334–1336. https://doi.org/10.1373/clinchem.2011.165019
  12. Nirwani, Hartiti, T., & Faruq, Z. H. (2018). Analisis akurasi dan presisi alat hematology analyzer abx pentra xl 80 di laboratorium rumah sakit roemani muhammadiyah semarang. Analisis Akurasi Dan Presisi Alat Hematology analyzer Abx Pentra Xl 80 Di Laboratorium Rumah Sakit Roemani Muhammadiyah Semarang. http://repository.unimus.ac.id
  13. Nurgiyantoro, B., & Dkk. (2017). Statistik Terapan Untuk Penelitian Ilmu Sosial. Gadjah Mada University Press.
  14. Nurrachmat, H. (2005). Perbedaan jumlah eritrosit, leukosit, dan trombosit pada pemberian antikoagulan EDTA konvensional dengan EDTA vacutainer. In Tesis. Bagian Patologi Klinik FK Undip, Semarang.
  15. Praptomo, A. J. (2018). Pengendalian Mutu Laboratorium Medis. Deepublish.
  16. Siregar, M. T., Wulan, W. S., Setiawan, D., & Nuryati, A. (2018). Bahan Ajar Teknologi Laboratorium Medik : Kendali Mutu. Badan Pengembangan dan Pemberdayaan Sumber daya Manusia Kementrian Kesehatan RI.
  17. Sriwati, C. (2018). Perbedaan Kadar Hemoglobin Pada Sampel Yang Segera Diperiksa Dan Ditunda 3 Jam. Universitas Muhammadiyah Semarang.
  18. Verbrugge, S. E., & Huisman, A. (2015). Verification and Standardization of Blood Cell Counters for Routine Clinical Laboratory Tests. Clinics in Laboratory Medicine, 35(1), 183–196. https://doi.org/10.1016/j.cll.2014.10.008
  19. XN-450/XN-430, S. (n.d.). Sysmex XN-450/XN-430 Automated Hematology analyzer CLSI Procedure. Document Number 1235-LSS, Rev.4, September 2018.
  20. Yumaroh, S., Ariyadi, T., & Faruq, Z. H. (2018). Perbedaan Kadar Hematokrit Darah Edta Disimpan Suhu Kamar Dan Suhu Lemari Pendingin Selama 6 Jam Dan 18 Jam. Universitas Muhammadiyah Semarang.
  21. Zini, G. (2014). Stability of complete blood count parameters with storage: Toward defined specifications for different diagnostic applications. International Journal of Laboratory Hematology, 36(2), 111–113. https://doi.org/10.1111/ijlh.12181